The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus
The increasing experimental evidences suggest that there are some forms of specific acquired immunity in invertebrates, but the underlying mechanism is not fully understood. In the present study, Pacific oyster (Crassostrea gigas) stimulated primarily by heat-killed Vibrio splendidus displayed stron...
Published in: | Developmental & Comparative Immunology |
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2014
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Online Access: | http://ir.qdio.ac.cn/handle/337002/24186 https://doi.org/10.1016/j.dci.2014.02.015 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/24186 2023-05-15T15:57:55+02:00 The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus Zhang, Tao Qiu, Limei Sun, Zhibin Wang, Lingling Zhou, Zhi Liu, Rui Yue, Feng Sun, Rui Song, Linsheng Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. 2014-07-01 http://ir.qdio.ac.cn/handle/337002/24186 https://doi.org/10.1016/j.dci.2014.02.015 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/24186 doi:10.1016/j.dci.2014.02.015 cn.org.cspace.api.content.CopyrightPolicy@1b4ad1e2 Crassostrea Gigas Immune Priming Phagocytosis Hemocytes Regeneration Hemopoiesis Immunology Zoology Article 期刊论文 2014 ftchinacasciocas https://doi.org/10.1016/j.dci.2014.02.015 2022-06-27T05:36:49Z The increasing experimental evidences suggest that there are some forms of specific acquired immunity in invertebrates, but the underlying mechanism is not fully understood. In the present study, Pacific oyster (Crassostrea gigas) stimulated primarily by heat-killed Vibrio splendidus displayed stronger immune responses at cellular and molecular levels when they encountered the secondary challenge of live V. splendidus. The total hemocyte counts (THC) increased significantly after the primary stimulation of heat-killed V. splendidus, and it increased even higher (p < 0.01) and reached the peak earlier (at 6 h) after the secondary challenge with live V. splendidus compared with that of the primary stimulation. The number of new generated circulating hemocytes increased dramatically (p < 0.01) at 6 h after the pre-stimulated oysters received the secondary stimulation with live V. splendidus, and the phagocytic rate was also enhanced significantly (p < 0.01) at 12 h after the secondary stimulation. Meanwhile, the enhanced phagocytosis of hemocytes was highly specific for V. splendidus and they could distinguish Vibrio anguillarum, Vibrio coralliilyticus, Yarrowia lipolytica, and Micrococcus luteus efficiently. In addition, the mRNA expression of 12 candidate genes related to phagocytosis and hematopoiesis were also monitored, and the expression levels of Cgintegrin, CgPI3K (phosphatidylinositol 3-kinase), CgRho J, CgMAPKK (mitogen-activated protein kinase kinase), CgRab32, CgNADPH (nicotinamide adenine dinucleotide phosphate) oxidase, CgRunx1 and CgBMP7 (bone morphogenetic protein 7) in the hemocytes of pre-stimulated oysters after the secondary stimulation of V. splendidus were higher (p < 0.01) than that after the primary stimulation, but there was no statistically significant changes for the genes of CgPKC (protein kinase C), CgMyosin, CgActin, and CgGATA 3. These results collectively suggested that the primary stimulation of V. splendidus led to immune priming in oyster with specifically enhanced ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Developmental & Comparative Immunology 45 1 141 150 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea Gigas Immune Priming Phagocytosis Hemocytes Regeneration Hemopoiesis Immunology Zoology |
spellingShingle |
Crassostrea Gigas Immune Priming Phagocytosis Hemocytes Regeneration Hemopoiesis Immunology Zoology Zhang, Tao Qiu, Limei Sun, Zhibin Wang, Lingling Zhou, Zhi Liu, Rui Yue, Feng Sun, Rui Song, Linsheng Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus |
topic_facet |
Crassostrea Gigas Immune Priming Phagocytosis Hemocytes Regeneration Hemopoiesis Immunology Zoology |
description |
The increasing experimental evidences suggest that there are some forms of specific acquired immunity in invertebrates, but the underlying mechanism is not fully understood. In the present study, Pacific oyster (Crassostrea gigas) stimulated primarily by heat-killed Vibrio splendidus displayed stronger immune responses at cellular and molecular levels when they encountered the secondary challenge of live V. splendidus. The total hemocyte counts (THC) increased significantly after the primary stimulation of heat-killed V. splendidus, and it increased even higher (p < 0.01) and reached the peak earlier (at 6 h) after the secondary challenge with live V. splendidus compared with that of the primary stimulation. The number of new generated circulating hemocytes increased dramatically (p < 0.01) at 6 h after the pre-stimulated oysters received the secondary stimulation with live V. splendidus, and the phagocytic rate was also enhanced significantly (p < 0.01) at 12 h after the secondary stimulation. Meanwhile, the enhanced phagocytosis of hemocytes was highly specific for V. splendidus and they could distinguish Vibrio anguillarum, Vibrio coralliilyticus, Yarrowia lipolytica, and Micrococcus luteus efficiently. In addition, the mRNA expression of 12 candidate genes related to phagocytosis and hematopoiesis were also monitored, and the expression levels of Cgintegrin, CgPI3K (phosphatidylinositol 3-kinase), CgRho J, CgMAPKK (mitogen-activated protein kinase kinase), CgRab32, CgNADPH (nicotinamide adenine dinucleotide phosphate) oxidase, CgRunx1 and CgBMP7 (bone morphogenetic protein 7) in the hemocytes of pre-stimulated oysters after the secondary stimulation of V. splendidus were higher (p < 0.01) than that after the primary stimulation, but there was no statistically significant changes for the genes of CgPKC (protein kinase C), CgMyosin, CgActin, and CgGATA 3. These results collectively suggested that the primary stimulation of V. splendidus led to immune priming in oyster with specifically enhanced ... |
format |
Article in Journal/Newspaper |
author |
Zhang, Tao Qiu, Limei Sun, Zhibin Wang, Lingling Zhou, Zhi Liu, Rui Yue, Feng Sun, Rui Song, Linsheng Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
author_facet |
Zhang, Tao Qiu, Limei Sun, Zhibin Wang, Lingling Zhou, Zhi Liu, Rui Yue, Feng Sun, Rui Song, Linsheng Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. |
author_sort |
Zhang, Tao |
title |
The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus |
title_short |
The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus |
title_full |
The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus |
title_fullStr |
The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus |
title_full_unstemmed |
The specifically enhanced cellular immune responses in Pacific oyster (Crassostrea gigas) against secondary challenge with Vibrio splendidus |
title_sort |
specifically enhanced cellular immune responses in pacific oyster (crassostrea gigas) against secondary challenge with vibrio splendidus |
publishDate |
2014 |
url |
http://ir.qdio.ac.cn/handle/337002/24186 https://doi.org/10.1016/j.dci.2014.02.015 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/24186 doi:10.1016/j.dci.2014.02.015 |
op_rights |
cn.org.cspace.api.content.CopyrightPolicy@1b4ad1e2 |
op_doi |
https://doi.org/10.1016/j.dci.2014.02.015 |
container_title |
Developmental & Comparative Immunology |
container_volume |
45 |
container_issue |
1 |
container_start_page |
141 |
op_container_end_page |
150 |
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1766393617166893056 |