The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas
Tumor necrosis factor (TNF) is one of the most important cytokines involved in many processes in both vertebrate and invertebrate. In the present study, a new tumor necrosis factor with a typical TNF domain was identified in oyster Crassostrea gigas (designated CgTNF-1). CgTNF-1 shared low sequence...
Published in: | Developmental & Comparative Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/24178 2023-05-15T15:58:25+02:00 The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas Sun, Ying Zho, Zhi Wang, Lingling Yang, Chuanyan Jianga, Shuai Song, Linsheng Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. 2014-08-01 http://ir.qdio.ac.cn/handle/337002/24178 https://doi.org/10.1016/j.dci.2014.03.007 英语 eng DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/24178 doi:10.1016/j.dci.2014.03.007 cn.org.cspace.api.content.CopyrightPolicy@1b4ad1e2 Tumor Necrosis Factor Immunomodulation Apoptosis Phagocytosis Oyster Immunology Zoology Article 期刊论文 2014 ftchinacasciocas https://doi.org/10.1016/j.dci.2014.03.007 2022-06-27T05:36:49Z Tumor necrosis factor (TNF) is one of the most important cytokines involved in many processes in both vertebrate and invertebrate. In the present study, a new tumor necrosis factor with a typical TNF domain was identified in oyster Crassostrea gigas (designated CgTNF-1). CgTNF-1 shared low sequence identity and similarity with the TNF superfamily members from other vertebrate and invertebrate. After LPS stimulation, the mRNA expression of CgTNF-1 in haemocytes increased significantly and peaked at 12 h (1.39 +/- 0.12, P < 0.05) post treatment, and the expression of CgTNF-1 protein in haemolymph also increased obviously during 6-12 h. When the oyster haemocytes were incubated with rCgTNF-1, its apoptosis and phagocytosis rate were both effectively induced and peaked at 12 h post the treatment of rCgTNF-1 with the concentration of 100 ng mL(-1) (23.3 +/- 3%, P < 0.01), 50 ng mL(-1) (5.3 +/- 0.6%, P < 0.05) and 10 ng mL(-1) (6.7 +/- 1.2%, P < 0.05), respectively. After the co-stimulation of LPS and rCgTNF-1, the apoptosis and phagocytosis rate of oyster haemocytes, and the activities of PO and lysozyme in the haemolymph all increased significantly, and reached the peak at 12 h (apoptosis rate 26.7 +/- 1.5%, P < 0.01), 12 h (phagocytosis rate 8.3 +/- 0.6%, P < 0.01), 6h (PO 1.11 +/- 0.01 U mg prot(-1), P < 0.01) and 12h (lysozyme 168.9 +/- 8.3 U mg prot(-1), P < 0.05), respectively, which were significantly higher than that in the LPS group. Furthermore, the antibacteria activity in the LPS + TNF group was significantly higher than that in the LPS group during 612 h. All the results collectively indicated that CgTNF-1 was involved in the oyster immunity and played a crucial role in the modulation of immune response including apoptosis and phagocytosis of haemocytes, and regulation of antibacterial activity as well as the activation of immune relevant enzymes. (C) 2014 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 45 2 291 299 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Tumor Necrosis Factor Immunomodulation Apoptosis Phagocytosis Oyster Immunology Zoology |
spellingShingle |
Tumor Necrosis Factor Immunomodulation Apoptosis Phagocytosis Oyster Immunology Zoology Sun, Ying Zho, Zhi Wang, Lingling Yang, Chuanyan Jianga, Shuai Song, Linsheng Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas |
topic_facet |
Tumor Necrosis Factor Immunomodulation Apoptosis Phagocytosis Oyster Immunology Zoology |
description |
Tumor necrosis factor (TNF) is one of the most important cytokines involved in many processes in both vertebrate and invertebrate. In the present study, a new tumor necrosis factor with a typical TNF domain was identified in oyster Crassostrea gigas (designated CgTNF-1). CgTNF-1 shared low sequence identity and similarity with the TNF superfamily members from other vertebrate and invertebrate. After LPS stimulation, the mRNA expression of CgTNF-1 in haemocytes increased significantly and peaked at 12 h (1.39 +/- 0.12, P < 0.05) post treatment, and the expression of CgTNF-1 protein in haemolymph also increased obviously during 6-12 h. When the oyster haemocytes were incubated with rCgTNF-1, its apoptosis and phagocytosis rate were both effectively induced and peaked at 12 h post the treatment of rCgTNF-1 with the concentration of 100 ng mL(-1) (23.3 +/- 3%, P < 0.01), 50 ng mL(-1) (5.3 +/- 0.6%, P < 0.05) and 10 ng mL(-1) (6.7 +/- 1.2%, P < 0.05), respectively. After the co-stimulation of LPS and rCgTNF-1, the apoptosis and phagocytosis rate of oyster haemocytes, and the activities of PO and lysozyme in the haemolymph all increased significantly, and reached the peak at 12 h (apoptosis rate 26.7 +/- 1.5%, P < 0.01), 12 h (phagocytosis rate 8.3 +/- 0.6%, P < 0.01), 6h (PO 1.11 +/- 0.01 U mg prot(-1), P < 0.01) and 12h (lysozyme 168.9 +/- 8.3 U mg prot(-1), P < 0.05), respectively, which were significantly higher than that in the LPS group. Furthermore, the antibacteria activity in the LPS + TNF group was significantly higher than that in the LPS group during 612 h. All the results collectively indicated that CgTNF-1 was involved in the oyster immunity and played a crucial role in the modulation of immune response including apoptosis and phagocytosis of haemocytes, and regulation of antibacterial activity as well as the activation of immune relevant enzymes. (C) 2014 Elsevier Ltd. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Sun, Ying Zho, Zhi Wang, Lingling Yang, Chuanyan Jianga, Shuai Song, Linsheng Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_facet |
Sun, Ying Zho, Zhi Wang, Lingling Yang, Chuanyan Jianga, Shuai Song, Linsheng Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_sort |
Sun, Ying |
title |
The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas |
title_short |
The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas |
title_full |
The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas |
title_fullStr |
The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas |
title_full_unstemmed |
The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas |
title_sort |
immunomodulation of a novel tumor necrosis factor (cgtnf-1) in oyster crassostrea gigas |
publishDate |
2014 |
url |
http://ir.qdio.ac.cn/handle/337002/24178 https://doi.org/10.1016/j.dci.2014.03.007 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/24178 doi:10.1016/j.dci.2014.03.007 |
op_rights |
cn.org.cspace.api.content.CopyrightPolicy@1b4ad1e2 |
op_doi |
https://doi.org/10.1016/j.dci.2014.03.007 |
container_title |
Developmental & Comparative Immunology |
container_volume |
45 |
container_issue |
2 |
container_start_page |
291 |
op_container_end_page |
299 |
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1766394163635421184 |