Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens
Pseudomonas fluorescens is a Gram-negative bacterium that can infect a wide range of farmed fish. However, very little is known about the virulence mechanism of P. fluorescens as a fish pathogen. In this study, we identified and analyzed 3 TonB-dependent outer membrane receptors (TDRs) from a pathog...
Published in: | Diseases of Aquatic Organisms |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2014
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/24122 https://doi.org/10.3354/dao02771 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/24122 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/24122 2023-05-15T18:15:49+02:00 Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens Zhang, Shu-ren Zhang, Lu Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. 2014-08-11 http://ir.qdio.ac.cn/handle/337002/24122 https://doi.org/10.3354/dao02771 英语 eng DISEASES OF AQUATIC ORGANISMS http://ir.qdio.ac.cn/handle/337002/24122 doi:10.3354/dao02771 cn.org.cspace.api.content.CopyrightPolicy@1b4ad1e2 Pseudomonas Fluorescens Tonb-dependent Receptor Iron Virulence Vaccine Fisheries Veterinary Sciences Article 期刊论文 2014 ftchinacasciocas https://doi.org/10.3354/dao02771 2022-06-27T05:36:49Z Pseudomonas fluorescens is a Gram-negative bacterium that can infect a wide range of farmed fish. However, very little is known about the virulence mechanism of P. fluorescens as a fish pathogen. In this study, we identified and analyzed 3 TonB-dependent outer membrane receptors (TDRs) from a pathogenic P. fluorescens strain isolated from fish. In silico analysis revealed that all 3 proteins (named Tdr1 to 3) possess structural domains typical of TDRs. Quantitative real time RT-PCR analysis showed that tdr1, tdr2, and tdr3 expressions were upregulated under iron-depleted conditions. Compared to the wild type, mutants defective in tdr1, tdr2, and tdr3 were retarded in growth to different extents. Infection in a turbot Scophthalmus maximus model showed that all 3 mutants were impaired in their ability to desseminate into and colonize host tissues. In addition, the tdr1 and tdr3 mutants exhibited significantly reduced virulence. When used as subunit vaccines, purified recombinant proteins of Tdr1, Tdr2, and, in particular, Tdr3 elicited significant protection in turbot against lethal P. fluorescens challenge. The vaccinated fish produced specific serum antibodies, which, when incubated with P. fluorescens, blocked infection of P. fluorescens in fish cells. Together these results indicate that Tdr1, Tdr2, and Tdr3 are iron-regulated factors that participate in bacterial virulence and induce protective immunity as subunit vaccines. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Diseases of Aquatic Organisms 110 3 181 191 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pseudomonas Fluorescens Tonb-dependent Receptor Iron Virulence Vaccine Fisheries Veterinary Sciences |
spellingShingle |
Pseudomonas Fluorescens Tonb-dependent Receptor Iron Virulence Vaccine Fisheries Veterinary Sciences Zhang, Shu-ren Zhang, Lu Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens |
topic_facet |
Pseudomonas Fluorescens Tonb-dependent Receptor Iron Virulence Vaccine Fisheries Veterinary Sciences |
description |
Pseudomonas fluorescens is a Gram-negative bacterium that can infect a wide range of farmed fish. However, very little is known about the virulence mechanism of P. fluorescens as a fish pathogen. In this study, we identified and analyzed 3 TonB-dependent outer membrane receptors (TDRs) from a pathogenic P. fluorescens strain isolated from fish. In silico analysis revealed that all 3 proteins (named Tdr1 to 3) possess structural domains typical of TDRs. Quantitative real time RT-PCR analysis showed that tdr1, tdr2, and tdr3 expressions were upregulated under iron-depleted conditions. Compared to the wild type, mutants defective in tdr1, tdr2, and tdr3 were retarded in growth to different extents. Infection in a turbot Scophthalmus maximus model showed that all 3 mutants were impaired in their ability to desseminate into and colonize host tissues. In addition, the tdr1 and tdr3 mutants exhibited significantly reduced virulence. When used as subunit vaccines, purified recombinant proteins of Tdr1, Tdr2, and, in particular, Tdr3 elicited significant protection in turbot against lethal P. fluorescens challenge. The vaccinated fish produced specific serum antibodies, which, when incubated with P. fluorescens, blocked infection of P. fluorescens in fish cells. Together these results indicate that Tdr1, Tdr2, and Tdr3 are iron-regulated factors that participate in bacterial virulence and induce protective immunity as subunit vaccines. |
format |
Article in Journal/Newspaper |
author |
Zhang, Shu-ren Zhang, Lu Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_facet |
Zhang, Shu-ren Zhang, Lu Sun, Li Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
author_sort |
Zhang, Shu-ren |
title |
Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens |
title_short |
Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens |
title_full |
Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens |
title_fullStr |
Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens |
title_full_unstemmed |
Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens |
title_sort |
identification and analysis of three virulence-associated tonb-dependent outer membrane receptors of pseudomonas fluorescens |
publishDate |
2014 |
url |
http://ir.qdio.ac.cn/handle/337002/24122 https://doi.org/10.3354/dao02771 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
DISEASES OF AQUATIC ORGANISMS http://ir.qdio.ac.cn/handle/337002/24122 doi:10.3354/dao02771 |
op_rights |
cn.org.cspace.api.content.CopyrightPolicy@1b4ad1e2 |
op_doi |
https://doi.org/10.3354/dao02771 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
110 |
container_issue |
3 |
container_start_page |
181 |
op_container_end_page |
191 |
_version_ |
1766189054983929856 |