Enhancing the culturability of bacteria from the gastrointestinal tract of farmed adult turbot Scophthalmus maximus

Eighteen agar media were tested for the culture of gut-associated bacteria from farmed adult turbot (Scophthalmus maximus), including 16 agar media with or without 1% gastrointestinal (GI) supernatant, or with 2% or 4% GI supernatant. A total of 1 711 colonies were analyzed and 24 operational taxono...

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Bibliographic Details
Published in:Chinese Journal of Oceanology and Limnology
Main Authors: Xing Mengxin, Hou Zhanhui, Qu Yanmei, Liu Bin, Liu, B (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
Format: Article in Journal/Newspaper
Language:English
Published: 2014
Subjects:
Turbot
Gastrointestinal Supernatant
16s Rrna Gene
Operational Taxonomic Units (Otus)
Cultivation Efficiency
Marine & Freshwater Biology
Oceanography
Scophthalmus maximus
Online Access:http://ir.qdio.ac.cn/handle/337002/24110
https://doi.org/10.1007/s00343-014-3099-1
Description
Summary:Eighteen agar media were tested for the culture of gut-associated bacteria from farmed adult turbot (Scophthalmus maximus), including 16 agar media with or without 1% gastrointestinal (GI) supernatant, or with 2% or 4% GI supernatant. A total of 1 711 colonies were analyzed and 24 operational taxonomic units (OTUs) were identified. The greatest bacterial diversity was isolated on Zobell 2216E/Zobell 2216E+ agar media, whereas MRS/MRS+ agar media produced a low diversity of colonies. Agar media with GI supernatant (1%, 2%, or 4%) showed increased diversity and yielded different profiles of OTUs from the corresponding original media, suggesting that GI supernatant provides substances that enhance the culture efficiency of bacteria from the turbot GI tract. The large majority of the colonies (82%) were gamma-Proteobacteria, whereas 15.6% and 2.4% of colonies were Firmicutes and Actinobacteria, respectively. At the genus level, 49.4% of all colonies were assigned to Vibrio. Other potential pathogens, including Pseudomonas, Photobacterium, and Enterobacter, and potential probiotics, including Bacillus, Paenibacillus, and Pseudomonas, were also isolated on agar media. Most cultured bacteria belonged to species that were first described in the turbot GI tract. The impact of these species on turbot physiology and health should be investigated further.

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