Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae
BackgroundMitochondrial genomes have become a powerful tool for studying molecular genetics and phylogeny of mollusks. Currently, the position of Modiolinae within Mytilidae and the taxonomic and phylogenetic relationships within Modiolinae were still controversial. This study focuses on the complet...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/186252 2024-09-30T14:38:34+00:00 Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae Zhu, Yi Yan, Shaojing Ma, Peizhen Zhang, Yifei Zuo, Chenxia Ma, Xiaojie Zhang, Zhen 2024-12-01 http://ir.qdio.ac.cn/handle/337002/186251 http://ir.qdio.ac.cn/handle/337002/186252 https://doi.org/10.1007/s11033-024-09767-0 英语 eng SPRINGER MOLECULAR BIOLOGY REPORTS http://ir.qdio.ac.cn/handle/337002/186251 http://ir.qdio.ac.cn/handle/337002/186252 doi:10.1007/s11033-024-09767-0 Mytilidae Modiolinae Mitochondrial genome Phylogeny Genetic distance Biochemistry & Molecular Biology PHYLOGENETIC ANALYSIS NUCLEOTIDE-SEQUENCE MOLLUSCA MYTILIDAE RNA GENE DNA MUSSEL BIVALVIA ORGANIZATION REPLICATION PERFORMANCE 期刊论文 2024 ftchinacasciocas https://doi.org/10.1007/s11033-024-09767-0 2024-09-05T23:42:51Z BackgroundMitochondrial genomes have become a powerful tool for studying molecular genetics and phylogeny of mollusks. Currently, the position of Modiolinae within Mytilidae and the taxonomic and phylogenetic relationships within Modiolinae were still controversial. This study focuses on the complete mitochondrial genomes of two species: Modiolus modulaides (R & ouml;ding, 1798) and Modiolus auriculatus Krauss, 1848, which have not been sequenced before.Methods and resultsWe assembled and characterized the mitochondrial genomes of M. modulaides and M. auriculatus and then analyzed the phylogenetic relationships. The mitochondrial genomes of M. modulaides and M. auriculatus were 15,422 bp and 16,027 bp, respectively. Both of them were composed of 36 functional genes, including 12 protein-coding genes, 22 transfer RNAs, and 2 ribosomal RNAs. All protein-coding genes showed A + T bias, positive GC skews, and negative AT skews in nucleotide composition. Phylogenetic analysis based on the mitochondrial genomes showed that Modiolinae and Bathymodiolinae clustered together to form a sister relationship. Seven Modiolinae species were divided into two clades: L1 (M. modulaides, M. auriculatus and Modiolus philippinarum Hanley, 1843) and L2 [Modiolus modiolus (Linnaeus, 1758), Modiolus kurilensis Bernard, 1983, Modiolus nipponicus (Oyama, 1950), and Modiolus comptus (Sowerby III, 1915)]. The divergence time of the two clades was approximately 105.75 Ma. Furthermore, the transfer RNA gene rearrangement, longer genetic distance, and greater genetic differentiation were confirmed between the L1 and L2 clades, as well as differences in the external characteristics of the shells of the two clades.ConclusionsBased on the molecular data, it was speculated that species from the L1 clade might belong to other genera or new genera. This study provides molecular information for further taxonomic and phylogenetic studies of Mytilidae. Report Modiolus modiolus Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Molecular Biology Reports 51 1 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Mytilidae Modiolinae Mitochondrial genome Phylogeny Genetic distance Biochemistry & Molecular Biology PHYLOGENETIC ANALYSIS NUCLEOTIDE-SEQUENCE MOLLUSCA MYTILIDAE RNA GENE DNA MUSSEL BIVALVIA ORGANIZATION REPLICATION PERFORMANCE |
spellingShingle |
Mytilidae Modiolinae Mitochondrial genome Phylogeny Genetic distance Biochemistry & Molecular Biology PHYLOGENETIC ANALYSIS NUCLEOTIDE-SEQUENCE MOLLUSCA MYTILIDAE RNA GENE DNA MUSSEL BIVALVIA ORGANIZATION REPLICATION PERFORMANCE Zhu, Yi Yan, Shaojing Ma, Peizhen Zhang, Yifei Zuo, Chenxia Ma, Xiaojie Zhang, Zhen Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae |
topic_facet |
Mytilidae Modiolinae Mitochondrial genome Phylogeny Genetic distance Biochemistry & Molecular Biology PHYLOGENETIC ANALYSIS NUCLEOTIDE-SEQUENCE MOLLUSCA MYTILIDAE RNA GENE DNA MUSSEL BIVALVIA ORGANIZATION REPLICATION PERFORMANCE |
description |
BackgroundMitochondrial genomes have become a powerful tool for studying molecular genetics and phylogeny of mollusks. Currently, the position of Modiolinae within Mytilidae and the taxonomic and phylogenetic relationships within Modiolinae were still controversial. This study focuses on the complete mitochondrial genomes of two species: Modiolus modulaides (R & ouml;ding, 1798) and Modiolus auriculatus Krauss, 1848, which have not been sequenced before.Methods and resultsWe assembled and characterized the mitochondrial genomes of M. modulaides and M. auriculatus and then analyzed the phylogenetic relationships. The mitochondrial genomes of M. modulaides and M. auriculatus were 15,422 bp and 16,027 bp, respectively. Both of them were composed of 36 functional genes, including 12 protein-coding genes, 22 transfer RNAs, and 2 ribosomal RNAs. All protein-coding genes showed A + T bias, positive GC skews, and negative AT skews in nucleotide composition. Phylogenetic analysis based on the mitochondrial genomes showed that Modiolinae and Bathymodiolinae clustered together to form a sister relationship. Seven Modiolinae species were divided into two clades: L1 (M. modulaides, M. auriculatus and Modiolus philippinarum Hanley, 1843) and L2 [Modiolus modiolus (Linnaeus, 1758), Modiolus kurilensis Bernard, 1983, Modiolus nipponicus (Oyama, 1950), and Modiolus comptus (Sowerby III, 1915)]. The divergence time of the two clades was approximately 105.75 Ma. Furthermore, the transfer RNA gene rearrangement, longer genetic distance, and greater genetic differentiation were confirmed between the L1 and L2 clades, as well as differences in the external characteristics of the shells of the two clades.ConclusionsBased on the molecular data, it was speculated that species from the L1 clade might belong to other genera or new genera. This study provides molecular information for further taxonomic and phylogenetic studies of Mytilidae. |
format |
Report |
author |
Zhu, Yi Yan, Shaojing Ma, Peizhen Zhang, Yifei Zuo, Chenxia Ma, Xiaojie Zhang, Zhen |
author_facet |
Zhu, Yi Yan, Shaojing Ma, Peizhen Zhang, Yifei Zuo, Chenxia Ma, Xiaojie Zhang, Zhen |
author_sort |
Zhu, Yi |
title |
Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae |
title_short |
Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae |
title_full |
Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae |
title_fullStr |
Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae |
title_full_unstemmed |
Comparative mitochondrial genome analysis provides new insights into the classification of Modiolinae |
title_sort |
comparative mitochondrial genome analysis provides new insights into the classification of modiolinae |
publisher |
SPRINGER |
publishDate |
2024 |
url |
http://ir.qdio.ac.cn/handle/337002/186251 http://ir.qdio.ac.cn/handle/337002/186252 https://doi.org/10.1007/s11033-024-09767-0 |
genre |
Modiolus modiolus |
genre_facet |
Modiolus modiolus |
op_relation |
MOLECULAR BIOLOGY REPORTS http://ir.qdio.ac.cn/handle/337002/186251 http://ir.qdio.ac.cn/handle/337002/186252 doi:10.1007/s11033-024-09767-0 |
op_doi |
https://doi.org/10.1007/s11033-024-09767-0 |
container_title |
Molecular Biology Reports |
container_volume |
51 |
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1 |
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1811641205480488960 |