Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula
Prokaryotic diversity and community composition in the water column of eight stations (63 samples) around the Antarctic Peninsula of the Southern Ocean were investigated. Through pyrosequencing of the V3-V4 hypervariable regions of the 16S ribosomal RNA gene, we characterized 4 720 089 valid reads r...
Published in: | Acta Oceanologica Sinica |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/183648 2024-04-28T07:56:38+00:00 Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula Li, Jiang Zhao, Luying Gu, Xiaoqian Li, Chengxuan Zhang, Qian Fu, Liping Zhang, Ao 2023-09-01 http://ir.qdio.ac.cn/handle/337002/183648 https://doi.org/10.1007/s13131-023-2160-3 英语 eng SPRINGER ACTA OCEANOLOGICA SINICA http://ir.qdio.ac.cn/handle/337002/183648 doi:10.1007/s13131-023-2160-3 prokaryotic diversity 16S rRNA high-throughput sequencing (HTS) Southern Ocean Oceanography BACTERIAL COMMUNITY SUMMER BACTERIOPLANKTON DIVERSITY SURFACE NORTH ARCHAEAL NITROGEN ECOLOGY WINTER MICROORGANISMS 期刊论文 2023 ftchinacasciocas https://doi.org/10.1007/s13131-023-2160-3 2024-04-08T00:16:49Z Prokaryotic diversity and community composition in the water column of eight stations (63 samples) around the Antarctic Peninsula of the Southern Ocean were investigated. Through pyrosequencing of the V3-V4 hypervariable regions of the 16S ribosomal RNA gene, we characterized 4 720 089 valid reads representing 48 188 operational taxonomic units (OTUs, 97% similarity). The community was dominated by the phyla Pseudomonadota (original name: Proteobacteria, 47%), Oxyphotobacteria (26%), and Bacteroidota (original name: Bacteroidetes, 18%), which comprised an average of 91% of the total OTUs in all samples. The prokaryotic community composition varied vertically within the water column. Water column prokaryotic communities exhibited a clear depth profile, with higher microbial richness and higher diversity observed with increasing water depth. Cluster analysis of the community composition of water column samples exhibited a similar trend with depth. Correlation with environmental factors suggested distinct variation in prokaryotic community composition with changes in depth, salinity, temperature and dissolved oxygen levels. Functional prediction showed presence of active nitrogen, sulphur and methane metabolic cycles along the vertical transect of the studied region. These results will improve our knowledge of prokaryotic diversity and community composition at different depth of water column for better understanding of the microbial ecology and nutrient cycles in Antarctic Peninsula region of the Southern Ocean. Report Antarc* Antarctic Antarctic Peninsula Southern Ocean Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Acta Oceanologica Sinica 42 9 90 100 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
prokaryotic diversity 16S rRNA high-throughput sequencing (HTS) Southern Ocean Oceanography BACTERIAL COMMUNITY SUMMER BACTERIOPLANKTON DIVERSITY SURFACE NORTH ARCHAEAL NITROGEN ECOLOGY WINTER MICROORGANISMS |
spellingShingle |
prokaryotic diversity 16S rRNA high-throughput sequencing (HTS) Southern Ocean Oceanography BACTERIAL COMMUNITY SUMMER BACTERIOPLANKTON DIVERSITY SURFACE NORTH ARCHAEAL NITROGEN ECOLOGY WINTER MICROORGANISMS Li, Jiang Zhao, Luying Gu, Xiaoqian Li, Chengxuan Zhang, Qian Fu, Liping Zhang, Ao Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula |
topic_facet |
prokaryotic diversity 16S rRNA high-throughput sequencing (HTS) Southern Ocean Oceanography BACTERIAL COMMUNITY SUMMER BACTERIOPLANKTON DIVERSITY SURFACE NORTH ARCHAEAL NITROGEN ECOLOGY WINTER MICROORGANISMS |
description |
Prokaryotic diversity and community composition in the water column of eight stations (63 samples) around the Antarctic Peninsula of the Southern Ocean were investigated. Through pyrosequencing of the V3-V4 hypervariable regions of the 16S ribosomal RNA gene, we characterized 4 720 089 valid reads representing 48 188 operational taxonomic units (OTUs, 97% similarity). The community was dominated by the phyla Pseudomonadota (original name: Proteobacteria, 47%), Oxyphotobacteria (26%), and Bacteroidota (original name: Bacteroidetes, 18%), which comprised an average of 91% of the total OTUs in all samples. The prokaryotic community composition varied vertically within the water column. Water column prokaryotic communities exhibited a clear depth profile, with higher microbial richness and higher diversity observed with increasing water depth. Cluster analysis of the community composition of water column samples exhibited a similar trend with depth. Correlation with environmental factors suggested distinct variation in prokaryotic community composition with changes in depth, salinity, temperature and dissolved oxygen levels. Functional prediction showed presence of active nitrogen, sulphur and methane metabolic cycles along the vertical transect of the studied region. These results will improve our knowledge of prokaryotic diversity and community composition at different depth of water column for better understanding of the microbial ecology and nutrient cycles in Antarctic Peninsula region of the Southern Ocean. |
format |
Report |
author |
Li, Jiang Zhao, Luying Gu, Xiaoqian Li, Chengxuan Zhang, Qian Fu, Liping Zhang, Ao |
author_facet |
Li, Jiang Zhao, Luying Gu, Xiaoqian Li, Chengxuan Zhang, Qian Fu, Liping Zhang, Ao |
author_sort |
Li, Jiang |
title |
Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula |
title_short |
Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula |
title_full |
Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula |
title_fullStr |
Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula |
title_full_unstemmed |
Vertical microbial profiling of water column reveals prokaryotic communities and distribution features of Antarctic Peninsula |
title_sort |
vertical microbial profiling of water column reveals prokaryotic communities and distribution features of antarctic peninsula |
publisher |
SPRINGER |
publishDate |
2023 |
url |
http://ir.qdio.ac.cn/handle/337002/183648 https://doi.org/10.1007/s13131-023-2160-3 |
genre |
Antarc* Antarctic Antarctic Peninsula Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Southern Ocean |
op_relation |
ACTA OCEANOLOGICA SINICA http://ir.qdio.ac.cn/handle/337002/183648 doi:10.1007/s13131-023-2160-3 |
op_doi |
https://doi.org/10.1007/s13131-023-2160-3 |
container_title |
Acta Oceanologica Sinica |
container_volume |
42 |
container_issue |
9 |
container_start_page |
90 |
op_container_end_page |
100 |
_version_ |
1797584139399987200 |