Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)
Phosphate, as the main nutrient factor of lake eutrophication brought by pollutants discharged from agriculture and industry, is always considered to be a low-toxicity substance to aquatic animals. But the toxicity mechanism is unclear, and published information is limited. In this study, a 96 h acu...
Published in: | Science of The Total Environment |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/181340 2023-11-12T04:25:49+01:00 Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus) Qi, Ting Wu, Lele Yu, Jiachen Song, Zongcheng Liu, Feng Li, Jun Song, Xiefa Li, Xian 2023-02-25 http://ir.qdio.ac.cn/handle/337002/181340 https://doi.org/10.1016/j.scitotenv.2022.160430 英语 eng ELSEVIER SCIENCE OF THE TOTAL ENVIRONMENT http://ir.qdio.ac.cn/handle/337002/181340 doi:10.1016/j.scitotenv.2022.160430 Phosphate Toxicity Metabolomics Histopathological damage Gene expression Environmental Sciences & Ecology Environmental Sciences ACTIVATED PROTEIN-KINASE IMMUNE-RESPONSES STOCKING DENSITY CELLULAR-ENERGY MYOSIN-II WATER HEALTH EUTROPHICATION PERFORMANCE EXPRESSION 期刊论文 2023 ftchinacasciocas https://doi.org/10.1016/j.scitotenv.2022.160430 2023-10-15T16:07:14Z Phosphate, as the main nutrient factor of lake eutrophication brought by pollutants discharged from agriculture and industry, is always considered to be a low-toxicity substance to aquatic animals. But the toxicity mechanism is unclear, and published information is limited. In this study, a 96 h acute stress experiment was conducted on juvenile turbot (Scophthalmus maximus) with 0, 10, and 60 mg/L phosphate solutions. Metabonomic analysis revealed that low -dose phosphate (10 mg/L) disrupted glycerophospholipid, purine, and glycolipid metabolism, as well as the tricarbox-ylic acid (TCA) cycle in juveniles, even at 96 h of stress, which may lead to cell structure damage and signal recognition disorder between cells. Upregulated key genes in the main glycerophospholipid metabolic pathways, which matched the results of the metabolomic study, were detected. Furthermore, low-dose phosphate (10 mg/L) induced oxidative stress and immunotoxicity in fish, resulting in the raising of relevant genes expression such as cat and sod in liver and kidney. In addition, all phosphate-treated groups had induced lesions on gill tissue, as evidenced by pathological observations. In this study on toxic effects on and mechanism of phosphate in aquatic animals using metabolomics, gene expression, and histopathology, we confirm that acute low-dose phosphate could disrupt glycerophospholipid metabolism and induce stress in juvenile turbot. This can provide advice on the amount of phosphate accumulation for marine fish farming and on protecting species diversity and marine ecosystem from the point of view of phosphate toxicity to marine animals. Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Science of The Total Environment 861 160430 |
institution |
Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Phosphate Toxicity Metabolomics Histopathological damage Gene expression Environmental Sciences & Ecology Environmental Sciences ACTIVATED PROTEIN-KINASE IMMUNE-RESPONSES STOCKING DENSITY CELLULAR-ENERGY MYOSIN-II WATER HEALTH EUTROPHICATION PERFORMANCE EXPRESSION |
spellingShingle |
Phosphate Toxicity Metabolomics Histopathological damage Gene expression Environmental Sciences & Ecology Environmental Sciences ACTIVATED PROTEIN-KINASE IMMUNE-RESPONSES STOCKING DENSITY CELLULAR-ENERGY MYOSIN-II WATER HEALTH EUTROPHICATION PERFORMANCE EXPRESSION Qi, Ting Wu, Lele Yu, Jiachen Song, Zongcheng Liu, Feng Li, Jun Song, Xiefa Li, Xian Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus) |
topic_facet |
Phosphate Toxicity Metabolomics Histopathological damage Gene expression Environmental Sciences & Ecology Environmental Sciences ACTIVATED PROTEIN-KINASE IMMUNE-RESPONSES STOCKING DENSITY CELLULAR-ENERGY MYOSIN-II WATER HEALTH EUTROPHICATION PERFORMANCE EXPRESSION |
description |
Phosphate, as the main nutrient factor of lake eutrophication brought by pollutants discharged from agriculture and industry, is always considered to be a low-toxicity substance to aquatic animals. But the toxicity mechanism is unclear, and published information is limited. In this study, a 96 h acute stress experiment was conducted on juvenile turbot (Scophthalmus maximus) with 0, 10, and 60 mg/L phosphate solutions. Metabonomic analysis revealed that low -dose phosphate (10 mg/L) disrupted glycerophospholipid, purine, and glycolipid metabolism, as well as the tricarbox-ylic acid (TCA) cycle in juveniles, even at 96 h of stress, which may lead to cell structure damage and signal recognition disorder between cells. Upregulated key genes in the main glycerophospholipid metabolic pathways, which matched the results of the metabolomic study, were detected. Furthermore, low-dose phosphate (10 mg/L) induced oxidative stress and immunotoxicity in fish, resulting in the raising of relevant genes expression such as cat and sod in liver and kidney. In addition, all phosphate-treated groups had induced lesions on gill tissue, as evidenced by pathological observations. In this study on toxic effects on and mechanism of phosphate in aquatic animals using metabolomics, gene expression, and histopathology, we confirm that acute low-dose phosphate could disrupt glycerophospholipid metabolism and induce stress in juvenile turbot. This can provide advice on the amount of phosphate accumulation for marine fish farming and on protecting species diversity and marine ecosystem from the point of view of phosphate toxicity to marine animals. |
format |
Report |
author |
Qi, Ting Wu, Lele Yu, Jiachen Song, Zongcheng Liu, Feng Li, Jun Song, Xiefa Li, Xian |
author_facet |
Qi, Ting Wu, Lele Yu, Jiachen Song, Zongcheng Liu, Feng Li, Jun Song, Xiefa Li, Xian |
author_sort |
Qi, Ting |
title |
Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus) |
title_short |
Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus) |
title_full |
Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus) |
title_fullStr |
Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus) |
title_full_unstemmed |
Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus) |
title_sort |
acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (scophthalmus maximus) |
publisher |
ELSEVIER |
publishDate |
2023 |
url |
http://ir.qdio.ac.cn/handle/337002/181340 https://doi.org/10.1016/j.scitotenv.2022.160430 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
SCIENCE OF THE TOTAL ENVIRONMENT http://ir.qdio.ac.cn/handle/337002/181340 doi:10.1016/j.scitotenv.2022.160430 |
op_doi |
https://doi.org/10.1016/j.scitotenv.2022.160430 |
container_title |
Science of The Total Environment |
container_volume |
861 |
container_start_page |
160430 |
_version_ |
1782339990101950464 |