Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas
The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whol...
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Online Access: | http://ir.qdio.ac.cn/handle/337002/180897 http://ir.qdio.ac.cn/handle/337002/180898 https://doi.org/10.3389/fimmu.2022.928628 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/180898 2023-05-15T15:58:16+02:00 Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas Yao, Shanshan Li, Li Guan, Xudong He, Yan Jouaux, Aude Xu, Fei Guo, Ximing Zhang, Guofan Zhang, Linlin 2022-08-19 http://ir.qdio.ac.cn/handle/337002/180897 http://ir.qdio.ac.cn/handle/337002/180898 https://doi.org/10.3389/fimmu.2022.928628 英语 eng FRONTIERS MEDIA SA FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/180897 http://ir.qdio.ac.cn/handle/337002/180898 doi:10.3389/fimmu.2022.928628 Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance larval mortality molluscan aquaculture Immunology 期刊论文 2022 ftchinacasciocas https://doi.org/10.3389/fimmu.2022.928628 2023-01-16T16:14:35Z The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Frontiers in Immunology 13 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance larval mortality molluscan aquaculture Immunology |
spellingShingle |
Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance larval mortality molluscan aquaculture Immunology Yao, Shanshan Li, Li Guan, Xudong He, Yan Jouaux, Aude Xu, Fei Guo, Ximing Zhang, Guofan Zhang, Linlin Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
topic_facet |
Ostreid herpesvirus antiviral innate immunity oyster pooled-resequencing transcriptomic response disease resistance larval mortality molluscan aquaculture Immunology |
description |
The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance. |
format |
Report |
author |
Yao, Shanshan Li, Li Guan, Xudong He, Yan Jouaux, Aude Xu, Fei Guo, Ximing Zhang, Guofan Zhang, Linlin |
author_facet |
Yao, Shanshan Li, Li Guan, Xudong He, Yan Jouaux, Aude Xu, Fei Guo, Ximing Zhang, Guofan Zhang, Linlin |
author_sort |
Yao, Shanshan |
title |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_short |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_full |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_fullStr |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas |
title_sort |
pooled resequencing of larvae and adults reveals genomic variations associated with ostreid herpesvirus 1 resistance in the pacific oyster crassostrea gigas |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2022 |
url |
http://ir.qdio.ac.cn/handle/337002/180897 http://ir.qdio.ac.cn/handle/337002/180898 https://doi.org/10.3389/fimmu.2022.928628 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/180897 http://ir.qdio.ac.cn/handle/337002/180898 doi:10.3389/fimmu.2022.928628 |
op_doi |
https://doi.org/10.3389/fimmu.2022.928628 |
container_title |
Frontiers in Immunology |
container_volume |
13 |
_version_ |
1766393993695854592 |