The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus)
The peritoneal cavity plays an important role in the immune response, and intraperitoneal administration is an ideal vaccination route in fish. However, immune responses in the peritoneal cavity of teleost fish are still not completely characterized. This study characterized the morphology of perito...
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Online Access: | http://ir.qdio.ac.cn/handle/337002/180255 https://doi.org/10.3390/microorganisms10112175 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/180255 2023-05-15T18:10:00+02:00 The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus) Shi, Xueyan Chi, Heng Sun, Yuanyuan Tang, Xiaoqian Xing, Jing Sheng, Xiuzhen Zhan, Wenbin 2022-11-01 http://ir.qdio.ac.cn/handle/337002/180255 https://doi.org/10.3390/microorganisms10112175 英语 eng MDPI MICROORGANISMS http://ir.qdio.ac.cn/handle/337002/180255 doi:10.3390/microorganisms10112175 peritoneal cavity cells cell composition infection vaccination fish Microbiology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR L EXTRACELLULAR TRAPS SUBUNIT VACCINE DENDRITIC CELLS SPARUS-AURATA MACROPHAGES NEUTROPHILS INFLAMMATION EXPRESSION 期刊论文 2022 ftchinacasciocas https://doi.org/10.3390/microorganisms10112175 2023-01-16T16:14:20Z The peritoneal cavity plays an important role in the immune response, and intraperitoneal administration is an ideal vaccination route in fish. However, immune responses in the peritoneal cavity of teleost fish are still not completely characterized. This study characterized the morphology of peritoneal cavity cells (PerC cells) and their composition in flounder (Paralichthys olivaceus). Flow cytometric analysis of the resident PerC cells revealed two populations varying in granularity and size. One population, approximately 15.43% +/- 1.8%, was smaller with a lower granularity, designated as lymphocytes. The other population of the cells, about 78.17% +/- 3.52%, was larger with higher granularity and was designated as myeloid cells. The results of cytochemical staining and transmission electron microscopy indicated that peritoneal cavity in flounder normally contains a resident population of leukocytes dominated by granulocytes, macrophages, dendritic cells, and lymphocytes. The percentages of IgM(+), CD4(+), G-CSFR+, MHCII+, and CD83(+) leukocytes among PerC cells determined by flow cytometry were 3.13% +/- 0.4%, 2.83% +/- 0.53%, 21.12% +/- 1.44%, 27.11% +/- 3.30%, and 19.64% +/- 0.31%, respectively. Further, the changes in IgM(+), CD4(+), G-CSFR+, MHCII+, and CD83(+) leukocytes in flounder after Vibrio anguillarum infection and immunization were compared. The composition changed rapidly after the infection or vaccination treatment and included two stages, a non-specific stage dominated by phagocytes and a specific immune stage dominated by lymphocytes. Due to the virulence effectors of bacteria, the infected group exhibited a more intense and complicated PerC cells immune response than that of the immunization group. Following our previous study, this is the first report on the morphology and composition of PerC cells and the early activation of PerC cells in flounder response to V. anguillarum infection and vaccination. Report Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Microorganisms 10 11 2175 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
peritoneal cavity cells cell composition infection vaccination fish Microbiology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR L EXTRACELLULAR TRAPS SUBUNIT VACCINE DENDRITIC CELLS SPARUS-AURATA MACROPHAGES NEUTROPHILS INFLAMMATION EXPRESSION |
spellingShingle |
peritoneal cavity cells cell composition infection vaccination fish Microbiology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR L EXTRACELLULAR TRAPS SUBUNIT VACCINE DENDRITIC CELLS SPARUS-AURATA MACROPHAGES NEUTROPHILS INFLAMMATION EXPRESSION Shi, Xueyan Chi, Heng Sun, Yuanyuan Tang, Xiaoqian Xing, Jing Sheng, Xiuzhen Zhan, Wenbin The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus) |
topic_facet |
peritoneal cavity cells cell composition infection vaccination fish Microbiology TROUT ONCORHYNCHUS-MYKISS SALMO-SALAR L EXTRACELLULAR TRAPS SUBUNIT VACCINE DENDRITIC CELLS SPARUS-AURATA MACROPHAGES NEUTROPHILS INFLAMMATION EXPRESSION |
description |
The peritoneal cavity plays an important role in the immune response, and intraperitoneal administration is an ideal vaccination route in fish. However, immune responses in the peritoneal cavity of teleost fish are still not completely characterized. This study characterized the morphology of peritoneal cavity cells (PerC cells) and their composition in flounder (Paralichthys olivaceus). Flow cytometric analysis of the resident PerC cells revealed two populations varying in granularity and size. One population, approximately 15.43% +/- 1.8%, was smaller with a lower granularity, designated as lymphocytes. The other population of the cells, about 78.17% +/- 3.52%, was larger with higher granularity and was designated as myeloid cells. The results of cytochemical staining and transmission electron microscopy indicated that peritoneal cavity in flounder normally contains a resident population of leukocytes dominated by granulocytes, macrophages, dendritic cells, and lymphocytes. The percentages of IgM(+), CD4(+), G-CSFR+, MHCII+, and CD83(+) leukocytes among PerC cells determined by flow cytometry were 3.13% +/- 0.4%, 2.83% +/- 0.53%, 21.12% +/- 1.44%, 27.11% +/- 3.30%, and 19.64% +/- 0.31%, respectively. Further, the changes in IgM(+), CD4(+), G-CSFR+, MHCII+, and CD83(+) leukocytes in flounder after Vibrio anguillarum infection and immunization were compared. The composition changed rapidly after the infection or vaccination treatment and included two stages, a non-specific stage dominated by phagocytes and a specific immune stage dominated by lymphocytes. Due to the virulence effectors of bacteria, the infected group exhibited a more intense and complicated PerC cells immune response than that of the immunization group. Following our previous study, this is the first report on the morphology and composition of PerC cells and the early activation of PerC cells in flounder response to V. anguillarum infection and vaccination. |
format |
Report |
author |
Shi, Xueyan Chi, Heng Sun, Yuanyuan Tang, Xiaoqian Xing, Jing Sheng, Xiuzhen Zhan, Wenbin |
author_facet |
Shi, Xueyan Chi, Heng Sun, Yuanyuan Tang, Xiaoqian Xing, Jing Sheng, Xiuzhen Zhan, Wenbin |
author_sort |
Shi, Xueyan |
title |
The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus) |
title_short |
The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus) |
title_full |
The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus) |
title_fullStr |
The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus) |
title_full_unstemmed |
The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus) |
title_sort |
early peritoneal cavity immune response to vibrio anguillarum infection and to inactivated bacterium in olive flounder (paralichthys olivaceus) |
publisher |
MDPI |
publishDate |
2022 |
url |
http://ir.qdio.ac.cn/handle/337002/180255 https://doi.org/10.3390/microorganisms10112175 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
MICROORGANISMS http://ir.qdio.ac.cn/handle/337002/180255 doi:10.3390/microorganisms10112175 |
op_doi |
https://doi.org/10.3390/microorganisms10112175 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
11 |
container_start_page |
2175 |
_version_ |
1766182712494784512 |