Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica
BackgroundUnderstanding how organisms respond and adapt to environmental changes is central to evolutionary biology. As a sessile organism that has adapted to life in estuaries and intertidal zones, the eastern oyster Crassostrea virginica can tolerate wide fluctuations in temperature and salinity a...
Published in: | Frontiers in Marine Science |
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Language: | English |
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FRONTIERS MEDIA SA
2022
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Online Access: | http://ir.qdio.ac.cn/handle/337002/179618 https://doi.org/10.3389/fmars.2022.921877 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/179618 2023-05-15T17:54:22+02:00 Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica Li, Cui Wang, Haiyan Guo, Ximing 2022-06-17 http://ir.qdio.ac.cn/handle/337002/179618 https://doi.org/10.3389/fmars.2022.921877 英语 eng FRONTIERS MEDIA SA FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/179618 doi:10.3389/fmars.2022.921877 stress transcriptome cell cycle control apoptosis growth immune function epigenetic modification Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences DIFFERENTIAL EXPRESSION ANALYSIS TRANSCRIPTOME ANALYSIS REVEALS TUMOR-NECROSIS-FACTOR RNA-SEQ EXPERIMENTS AIR EXPOSURE PACIFIC OYSTER PROTEIN GENE TNF ADAPTATION 期刊论文 2022 ftchinacasciocas https://doi.org/10.3389/fmars.2022.921877 2022-12-14T16:02:09Z BackgroundUnderstanding how organisms respond and adapt to environmental changes is central to evolutionary biology. As a sessile organism that has adapted to life in estuaries and intertidal zones, the eastern oyster Crassostrea virginica can tolerate wide fluctuations in temperature and salinity and survive for weeks out of water. To understand the molecular mechanisms underlying the remarkable stress tolerance of the eastern oyster, we studied the transcriptomic changes induced by exposure to air and cold stress. Eastern oysters were maintained for 7 days under four conditions, namely, in seawater (normal) at 22 degrees C, in air at 22 degrees C, in seawater at 5 degrees C and in air at 5 degrees C, and then sampled for RNA sequencing. ResultsTranscriptomic analysis revealed that many genes involved in cell cycle progression and DNA replication were downregulated in oysters exposed to air and cold, which indicates that stress inhibits cell division. Exposure to air at 22 degrees C induced a concerted inhibition of apoptosis through the upregulation of expanded inhibitors of apoptosis and the downregulation of caspases. Interactions between TNF and NF-kappa B signalling implied a reduction in the inflammatory response and immune functions. Key genes for proline production, fatty acid synthesis and chromosomal proteins were upregulated during exposure to low temperatures, which suggested that proline accumulation, energy conservation, and epigenetic modification of chromosomes are important for coping with cold stress. The upregulation of melatonin, FMRFamide, and neural acetylcholine receptors indicate the significance of the neurohormonal regulation of homeostasis. ConclusionThese results show that air exposure and cold stress alter the expression of key genes for cell division, apoptosis, proline accumulation, fatty acid metabolism, neurohormonal signalling, and epigenetic modifications, suggesting regulation of these processes plays an important role in the stress response of the eastern oyster and possibly ... Report Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
stress transcriptome cell cycle control apoptosis growth immune function epigenetic modification Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences DIFFERENTIAL EXPRESSION ANALYSIS TRANSCRIPTOME ANALYSIS REVEALS TUMOR-NECROSIS-FACTOR RNA-SEQ EXPERIMENTS AIR EXPOSURE PACIFIC OYSTER PROTEIN GENE TNF ADAPTATION |
spellingShingle |
stress transcriptome cell cycle control apoptosis growth immune function epigenetic modification Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences DIFFERENTIAL EXPRESSION ANALYSIS TRANSCRIPTOME ANALYSIS REVEALS TUMOR-NECROSIS-FACTOR RNA-SEQ EXPERIMENTS AIR EXPOSURE PACIFIC OYSTER PROTEIN GENE TNF ADAPTATION Li, Cui Wang, Haiyan Guo, Ximing Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica |
topic_facet |
stress transcriptome cell cycle control apoptosis growth immune function epigenetic modification Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences DIFFERENTIAL EXPRESSION ANALYSIS TRANSCRIPTOME ANALYSIS REVEALS TUMOR-NECROSIS-FACTOR RNA-SEQ EXPERIMENTS AIR EXPOSURE PACIFIC OYSTER PROTEIN GENE TNF ADAPTATION |
description |
BackgroundUnderstanding how organisms respond and adapt to environmental changes is central to evolutionary biology. As a sessile organism that has adapted to life in estuaries and intertidal zones, the eastern oyster Crassostrea virginica can tolerate wide fluctuations in temperature and salinity and survive for weeks out of water. To understand the molecular mechanisms underlying the remarkable stress tolerance of the eastern oyster, we studied the transcriptomic changes induced by exposure to air and cold stress. Eastern oysters were maintained for 7 days under four conditions, namely, in seawater (normal) at 22 degrees C, in air at 22 degrees C, in seawater at 5 degrees C and in air at 5 degrees C, and then sampled for RNA sequencing. ResultsTranscriptomic analysis revealed that many genes involved in cell cycle progression and DNA replication were downregulated in oysters exposed to air and cold, which indicates that stress inhibits cell division. Exposure to air at 22 degrees C induced a concerted inhibition of apoptosis through the upregulation of expanded inhibitors of apoptosis and the downregulation of caspases. Interactions between TNF and NF-kappa B signalling implied a reduction in the inflammatory response and immune functions. Key genes for proline production, fatty acid synthesis and chromosomal proteins were upregulated during exposure to low temperatures, which suggested that proline accumulation, energy conservation, and epigenetic modification of chromosomes are important for coping with cold stress. The upregulation of melatonin, FMRFamide, and neural acetylcholine receptors indicate the significance of the neurohormonal regulation of homeostasis. ConclusionThese results show that air exposure and cold stress alter the expression of key genes for cell division, apoptosis, proline accumulation, fatty acid metabolism, neurohormonal signalling, and epigenetic modifications, suggesting regulation of these processes plays an important role in the stress response of the eastern oyster and possibly ... |
format |
Report |
author |
Li, Cui Wang, Haiyan Guo, Ximing |
author_facet |
Li, Cui Wang, Haiyan Guo, Ximing |
author_sort |
Li, Cui |
title |
Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica |
title_short |
Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica |
title_full |
Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica |
title_fullStr |
Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica |
title_full_unstemmed |
Regulation of the Cell Cycle, Apoptosis, and Proline Accumulation Plays an Important Role in the Stress Response of the Eastern Oyster Crassostrea Virginica |
title_sort |
regulation of the cell cycle, apoptosis, and proline accumulation plays an important role in the stress response of the eastern oyster crassostrea virginica |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2022 |
url |
http://ir.qdio.ac.cn/handle/337002/179618 https://doi.org/10.3389/fmars.2022.921877 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/179618 doi:10.3389/fmars.2022.921877 |
op_doi |
https://doi.org/10.3389/fmars.2022.921877 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
_version_ |
1766162116641816576 |