Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg)
The Pacific oyster, Crassostrea gigas (Thunberg), from the northwest Pacific Ocean has been introduced for aquaculture in many areas. Despite its economic importance, genetic dissection for growth, shell shape and yield traits have not been reported thoroughly. To explore single nucleotide polymorph...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/179450 2023-05-15T15:58:15+02:00 Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg) Liu, Sheng Li, Li Shi, Ruihui Wang, Wei Wu, Fucun Zhang, Guofan 2022-06-07 http://ir.qdio.ac.cn/handle/337002/179449 http://ir.qdio.ac.cn/handle/337002/179450 https://doi.org/10.1111/are.15903 英语 eng WILEY AQUACULTURE RESEARCH http://ir.qdio.ac.cn/handle/337002/179449 http://ir.qdio.ac.cn/handle/337002/179450 doi:10.1111/are.15903 candidate gene genome-wide association study growth-related traits Pacific oyster Fisheries 期刊论文 2022 ftchinacasciocas https://doi.org/10.1111/are.15903 2022-07-29T12:11:57Z The Pacific oyster, Crassostrea gigas (Thunberg), from the northwest Pacific Ocean has been introduced for aquaculture in many areas. Despite its economic importance, genetic dissection for growth, shell shape and yield traits have not been reported thoroughly. To explore single nucleotide polymorphisms (SNPs) associated with desired traits in Pacific oyster, a genome-wide association study (GWAS) was conducted based on the 190 K high-density SNP array. Growth, shell shape and yield traits, including shell height, condition index and dressing percentage, were studied. Moderate to high phenotypic correlations between shell height and other growth-related traits in the studied wild population were observed (r = 0.38-0.77, p < 0.05), and the dressing percentage was correlated with the condition index (r = 0.6, p < 0.05). The condition index had a moderate positive correlation with glycogen and fat content (r = 0.22-0.35, p < 0.05). The results of GWAS indicated that 11 SNPs were significantly associated with the studied traits. Shell height, body weight and soft body weight were all associated with a significant nonsynonymous SNP (scaffold731_92759), phenotypic variation explanations associated with this single SNP are 8.59%, 8.12% and 8.13%, respectively. This SNP is located in the protein-coding region of the serine protease inhibitor dipetalogastin, which is thought to be involved in food digestion and absorption. This polymorphism may affect the ability of dipetalogastin to inhibit serine proteases, thereby affecting the oyster's growth. This study identified new candidate genes and SNPs related to oyster growth, which is useful for marker-assisted selection. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Aquaculture Research 53 11 4007 4015 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
candidate gene genome-wide association study growth-related traits Pacific oyster Fisheries |
spellingShingle |
candidate gene genome-wide association study growth-related traits Pacific oyster Fisheries Liu, Sheng Li, Li Shi, Ruihui Wang, Wei Wu, Fucun Zhang, Guofan Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg) |
topic_facet |
candidate gene genome-wide association study growth-related traits Pacific oyster Fisheries |
description |
The Pacific oyster, Crassostrea gigas (Thunberg), from the northwest Pacific Ocean has been introduced for aquaculture in many areas. Despite its economic importance, genetic dissection for growth, shell shape and yield traits have not been reported thoroughly. To explore single nucleotide polymorphisms (SNPs) associated with desired traits in Pacific oyster, a genome-wide association study (GWAS) was conducted based on the 190 K high-density SNP array. Growth, shell shape and yield traits, including shell height, condition index and dressing percentage, were studied. Moderate to high phenotypic correlations between shell height and other growth-related traits in the studied wild population were observed (r = 0.38-0.77, p < 0.05), and the dressing percentage was correlated with the condition index (r = 0.6, p < 0.05). The condition index had a moderate positive correlation with glycogen and fat content (r = 0.22-0.35, p < 0.05). The results of GWAS indicated that 11 SNPs were significantly associated with the studied traits. Shell height, body weight and soft body weight were all associated with a significant nonsynonymous SNP (scaffold731_92759), phenotypic variation explanations associated with this single SNP are 8.59%, 8.12% and 8.13%, respectively. This SNP is located in the protein-coding region of the serine protease inhibitor dipetalogastin, which is thought to be involved in food digestion and absorption. This polymorphism may affect the ability of dipetalogastin to inhibit serine proteases, thereby affecting the oyster's growth. This study identified new candidate genes and SNPs related to oyster growth, which is useful for marker-assisted selection. |
format |
Report |
author |
Liu, Sheng Li, Li Shi, Ruihui Wang, Wei Wu, Fucun Zhang, Guofan |
author_facet |
Liu, Sheng Li, Li Shi, Ruihui Wang, Wei Wu, Fucun Zhang, Guofan |
author_sort |
Liu, Sheng |
title |
Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg) |
title_short |
Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg) |
title_full |
Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg) |
title_fullStr |
Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg) |
title_full_unstemmed |
Genome-wide association study for desirable traits in the Pacific oyster Crassostrea gigas (Thunberg) |
title_sort |
genome-wide association study for desirable traits in the pacific oyster crassostrea gigas (thunberg) |
publisher |
WILEY |
publishDate |
2022 |
url |
http://ir.qdio.ac.cn/handle/337002/179449 http://ir.qdio.ac.cn/handle/337002/179450 https://doi.org/10.1111/are.15903 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
AQUACULTURE RESEARCH http://ir.qdio.ac.cn/handle/337002/179449 http://ir.qdio.ac.cn/handle/337002/179450 doi:10.1111/are.15903 |
op_doi |
https://doi.org/10.1111/are.15903 |
container_title |
Aquaculture Research |
container_volume |
53 |
container_issue |
11 |
container_start_page |
4007 |
op_container_end_page |
4015 |
_version_ |
1766393985490747392 |