The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas
Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putativ...
Published in: | Developmental & Comparative Immunology |
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Language: | English |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/177025 https://doi.org/10.1016/j.dci.2021.104145 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/177025 2023-05-15T15:57:37+02:00 The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas Xin, Lusheng Liu, Chang Zhang, Huan Qiu, Limei Wang, Lingling Song, Linsheng 2021-10-01 http://ir.qdio.ac.cn/handle/337002/177025 https://doi.org/10.1016/j.dci.2021.104145 英语 eng ELSEVIER SCI LTD DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/177025 doi:10.1016/j.dci.2021.104145 Crassostrea gigas Cytokine IL-12 IL-12 p35 subunit Fisheries Immunology Veterinary Sciences Zoology INFLAMMATORY CYTOKINE IL-12 FAMILY EXPRESSION IDENTIFICATION P40 ACTIVATION PARALOGUES REVEALS CLONING INNATE 期刊论文 2021 ftchinacasciocas https://doi.org/10.1016/j.dci.2021.104145 2022-06-27T05:46:29Z Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putative peptide of 136 amino acids. There was a long four-helix chain in CgIL12p35L, which was similar as that in vertebrate IL-12 p35. Comparative genomic analysis showed that there were conservative kinds of syntenic genes flanked CgIL12p35L. The mRNA transcripts of CgIL12p35L were constitutively expressed in various tissues and its mRNA expression level in haemocytes increased significantly after bacteria challenge. The activity of haemolymph to eliminate bacteria from the oysters treated with recombinant CgIL12p35L protein (rCgIL12p35L) in vivo increased significantly. The results collectively indicated that the homolog of vertebrate IL-12 p35 subunit existed in oysters, and it was involved in immune defense against bacteria challenge. Report Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Developmental & Comparative Immunology 123 104145 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea gigas Cytokine IL-12 IL-12 p35 subunit Fisheries Immunology Veterinary Sciences Zoology INFLAMMATORY CYTOKINE IL-12 FAMILY EXPRESSION IDENTIFICATION P40 ACTIVATION PARALOGUES REVEALS CLONING INNATE |
spellingShingle |
Crassostrea gigas Cytokine IL-12 IL-12 p35 subunit Fisheries Immunology Veterinary Sciences Zoology INFLAMMATORY CYTOKINE IL-12 FAMILY EXPRESSION IDENTIFICATION P40 ACTIVATION PARALOGUES REVEALS CLONING INNATE Xin, Lusheng Liu, Chang Zhang, Huan Qiu, Limei Wang, Lingling Song, Linsheng The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas Cytokine IL-12 IL-12 p35 subunit Fisheries Immunology Veterinary Sciences Zoology INFLAMMATORY CYTOKINE IL-12 FAMILY EXPRESSION IDENTIFICATION P40 ACTIVATION PARALOGUES REVEALS CLONING INNATE |
description |
Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putative peptide of 136 amino acids. There was a long four-helix chain in CgIL12p35L, which was similar as that in vertebrate IL-12 p35. Comparative genomic analysis showed that there were conservative kinds of syntenic genes flanked CgIL12p35L. The mRNA transcripts of CgIL12p35L were constitutively expressed in various tissues and its mRNA expression level in haemocytes increased significantly after bacteria challenge. The activity of haemolymph to eliminate bacteria from the oysters treated with recombinant CgIL12p35L protein (rCgIL12p35L) in vivo increased significantly. The results collectively indicated that the homolog of vertebrate IL-12 p35 subunit existed in oysters, and it was involved in immune defense against bacteria challenge. |
format |
Report |
author |
Xin, Lusheng Liu, Chang Zhang, Huan Qiu, Limei Wang, Lingling Song, Linsheng |
author_facet |
Xin, Lusheng Liu, Chang Zhang, Huan Qiu, Limei Wang, Lingling Song, Linsheng |
author_sort |
Xin, Lusheng |
title |
The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas |
title_short |
The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas |
title_full |
The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas |
title_fullStr |
The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas |
title_full_unstemmed |
The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas |
title_sort |
characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster crassostrea gigas |
publisher |
ELSEVIER SCI LTD |
publishDate |
2021 |
url |
http://ir.qdio.ac.cn/handle/337002/177025 https://doi.org/10.1016/j.dci.2021.104145 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/177025 doi:10.1016/j.dci.2021.104145 |
op_doi |
https://doi.org/10.1016/j.dci.2021.104145 |
container_title |
Developmental & Comparative Immunology |
container_volume |
123 |
container_start_page |
104145 |
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1766393284131815424 |