Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus)
BackgroundAs flatfish, turbot undergo metamorphosis as part of their life cycle. In the larval stage, turbot live at the ocean surface, but after metamorphosis they move to deeper water and turn to benthic life. Thus, the light environment differs greatly between life stages. The visual system plays...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/176860 2023-05-15T18:15:52+02:00 Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus) Wang, Yunong Zhou, Li Wu, Lele Song, Changbin Ma, Xiaona Xu, Shihong Du, Tengfei Li, Xian Li, Jun 2021-06-07 http://ir.qdio.ac.cn/handle/337002/176860 https://doi.org/10.1186/s12862-021-01837-2 英语 eng BMC BMC ECOLOGY AND EVOLUTION http://ir.qdio.ac.cn/handle/337002/176860 doi:10.1186/s12862-021-01837-2 Turbot Benthic life Adaption Opsin Heterochronic shift Environmental Sciences & Ecology Evolutionary Biology Genetics & Heredity Ecology DETECTING POSITIVE SELECTION ADAPTIVE EVOLUTION COLOR-VISION FUNCTIONAL-CHARACTERIZATION MOLECULAR EVOLUTION DIVERGENCE ADAPTATION DIFFERENTIATION PRESERVATION DUPLICATION 期刊论文 2021 ftchinacasciocas https://doi.org/10.1186/s12862-021-01837-2 2022-06-27T05:46:25Z BackgroundAs flatfish, turbot undergo metamorphosis as part of their life cycle. In the larval stage, turbot live at the ocean surface, but after metamorphosis they move to deeper water and turn to benthic life. Thus, the light environment differs greatly between life stages. The visual system plays a great role in organic evolution, but reports of the relationship between the visual system and benthic life are rare. In this study, we reported the molecular and evolutionary analysis of opsin genes in turbot, and the heterochronic shifts in opsin expression during development.ResultsOur gene synteny analysis showed that subtype RH2C was not on the same gene cluster as the other four green-sensitive opsin genes (RH2) in turbot. It was translocated to chromosome 8 from chromosome 6. Based on branch-site test and spectral tuning sites analyses, E122Q and M207L substitutions in RH2C, which were found to be under positive selection, are closely related to the blue shift of optimum light sensitivities. And real-time PCR results indicated the dominant opsin gene shifted from red-sensitive (LWS) to RH2B1 during turbot development, which may lead to spectral sensitivity shifts to shorter wavelengths.ConclusionsThis is the first report that RH2C may be an important subtype of green opsin gene that was retained by turbot and possibly other flatfish species during evolution. Moreover, E122Q and M207L substitutions in RH2C may contribute to the survival of turbot in the bluish colored ocean. And heterochronic shifts in opsin expression may be an important strategy for turbot to adapt to benthic life. Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR BMC Ecology and Evolution 21 1 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Turbot Benthic life Adaption Opsin Heterochronic shift Environmental Sciences & Ecology Evolutionary Biology Genetics & Heredity Ecology DETECTING POSITIVE SELECTION ADAPTIVE EVOLUTION COLOR-VISION FUNCTIONAL-CHARACTERIZATION MOLECULAR EVOLUTION DIVERGENCE ADAPTATION DIFFERENTIATION PRESERVATION DUPLICATION |
spellingShingle |
Turbot Benthic life Adaption Opsin Heterochronic shift Environmental Sciences & Ecology Evolutionary Biology Genetics & Heredity Ecology DETECTING POSITIVE SELECTION ADAPTIVE EVOLUTION COLOR-VISION FUNCTIONAL-CHARACTERIZATION MOLECULAR EVOLUTION DIVERGENCE ADAPTATION DIFFERENTIATION PRESERVATION DUPLICATION Wang, Yunong Zhou, Li Wu, Lele Song, Changbin Ma, Xiaona Xu, Shihong Du, Tengfei Li, Xian Li, Jun Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus) |
topic_facet |
Turbot Benthic life Adaption Opsin Heterochronic shift Environmental Sciences & Ecology Evolutionary Biology Genetics & Heredity Ecology DETECTING POSITIVE SELECTION ADAPTIVE EVOLUTION COLOR-VISION FUNCTIONAL-CHARACTERIZATION MOLECULAR EVOLUTION DIVERGENCE ADAPTATION DIFFERENTIATION PRESERVATION DUPLICATION |
description |
BackgroundAs flatfish, turbot undergo metamorphosis as part of their life cycle. In the larval stage, turbot live at the ocean surface, but after metamorphosis they move to deeper water and turn to benthic life. Thus, the light environment differs greatly between life stages. The visual system plays a great role in organic evolution, but reports of the relationship between the visual system and benthic life are rare. In this study, we reported the molecular and evolutionary analysis of opsin genes in turbot, and the heterochronic shifts in opsin expression during development.ResultsOur gene synteny analysis showed that subtype RH2C was not on the same gene cluster as the other four green-sensitive opsin genes (RH2) in turbot. It was translocated to chromosome 8 from chromosome 6. Based on branch-site test and spectral tuning sites analyses, E122Q and M207L substitutions in RH2C, which were found to be under positive selection, are closely related to the blue shift of optimum light sensitivities. And real-time PCR results indicated the dominant opsin gene shifted from red-sensitive (LWS) to RH2B1 during turbot development, which may lead to spectral sensitivity shifts to shorter wavelengths.ConclusionsThis is the first report that RH2C may be an important subtype of green opsin gene that was retained by turbot and possibly other flatfish species during evolution. Moreover, E122Q and M207L substitutions in RH2C may contribute to the survival of turbot in the bluish colored ocean. And heterochronic shifts in opsin expression may be an important strategy for turbot to adapt to benthic life. |
format |
Report |
author |
Wang, Yunong Zhou, Li Wu, Lele Song, Changbin Ma, Xiaona Xu, Shihong Du, Tengfei Li, Xian Li, Jun |
author_facet |
Wang, Yunong Zhou, Li Wu, Lele Song, Changbin Ma, Xiaona Xu, Shihong Du, Tengfei Li, Xian Li, Jun |
author_sort |
Wang, Yunong |
title |
Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus) |
title_short |
Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus) |
title_full |
Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus) |
title_fullStr |
Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus) |
title_full_unstemmed |
Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus) |
title_sort |
evolutionary ecology of the visual opsin gene sequence and its expression in turbot (scophthalmus maximus) |
publisher |
BMC |
publishDate |
2021 |
url |
http://ir.qdio.ac.cn/handle/337002/176860 https://doi.org/10.1186/s12862-021-01837-2 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
BMC ECOLOGY AND EVOLUTION http://ir.qdio.ac.cn/handle/337002/176860 doi:10.1186/s12862-021-01837-2 |
op_doi |
https://doi.org/10.1186/s12862-021-01837-2 |
container_title |
BMC Ecology and Evolution |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766189102155169792 |