Screening of genes expressed in vivo after infection by Vibrio anguillarum M3
Aims: Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/1755 2024-09-15T18:34:02+00:00 Screening of genes expressed in vivo after infection by Vibrio anguillarum M3 Zou, Y. -X. Mo, Z. -L. Hao, B. Ye, X. -H. Guo, D. -S. Zhang, P. -J. Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2010-11-01 http://ir.qdio.ac.cn/handle/337002/1756 https://doi.org/10.1111/j.1472-765X.2010.02935.x 英语 eng LETTERS IN APPLIED MICROBIOLOGY Zou, Y. -X.; Mo, Z. -L.; Hao, B.; Ye, X. -H.; Guo, D. -S.; Zhang, P. -J.Screening of genes expressed in vivo after infection by Vibrio anguillarum M3,LETTERS IN APPLIED MICROBIOLOGY,2010,51(5):564-569 http://ir.qdio.ac.cn/handle/337002/1756 doi:10.1111/j.1472-765X.2010.02935.x In Vivo-expressed Genes Iviat Vibrio Anguillarum Biotechnology & Applied Microbiology Microbiology Article 期刊论文 2010 ftchinacasciocas https://doi.org/10.1111/j.1472-765X.2010.02935.x 2024-08-09T03:18:11Z Aims: Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents. Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Letters in Applied Microbiology 51 5 564 569 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
In Vivo-expressed Genes Iviat Vibrio Anguillarum Biotechnology & Applied Microbiology Microbiology |
spellingShingle |
In Vivo-expressed Genes Iviat Vibrio Anguillarum Biotechnology & Applied Microbiology Microbiology Zou, Y. -X. Mo, Z. -L. Hao, B. Ye, X. -H. Guo, D. -S. Zhang, P. -J. Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China Screening of genes expressed in vivo after infection by Vibrio anguillarum M3 |
topic_facet |
In Vivo-expressed Genes Iviat Vibrio Anguillarum Biotechnology & Applied Microbiology Microbiology |
description |
Aims: Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents. |
format |
Article in Journal/Newspaper |
author |
Zou, Y. -X. Mo, Z. -L. Hao, B. Ye, X. -H. Guo, D. -S. Zhang, P. -J. Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
author_facet |
Zou, Y. -X. Mo, Z. -L. Hao, B. Ye, X. -H. Guo, D. -S. Zhang, P. -J. Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
author_sort |
Zou, Y. -X. |
title |
Screening of genes expressed in vivo after infection by Vibrio anguillarum M3 |
title_short |
Screening of genes expressed in vivo after infection by Vibrio anguillarum M3 |
title_full |
Screening of genes expressed in vivo after infection by Vibrio anguillarum M3 |
title_fullStr |
Screening of genes expressed in vivo after infection by Vibrio anguillarum M3 |
title_full_unstemmed |
Screening of genes expressed in vivo after infection by Vibrio anguillarum M3 |
title_sort |
screening of genes expressed in vivo after infection by vibrio anguillarum m3 |
publishDate |
2010 |
url |
http://ir.qdio.ac.cn/handle/337002/1756 https://doi.org/10.1111/j.1472-765X.2010.02935.x |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
LETTERS IN APPLIED MICROBIOLOGY Zou, Y. -X.; Mo, Z. -L.; Hao, B.; Ye, X. -H.; Guo, D. -S.; Zhang, P. -J.Screening of genes expressed in vivo after infection by Vibrio anguillarum M3,LETTERS IN APPLIED MICROBIOLOGY,2010,51(5):564-569 http://ir.qdio.ac.cn/handle/337002/1756 doi:10.1111/j.1472-765X.2010.02935.x |
op_doi |
https://doi.org/10.1111/j.1472-765X.2010.02935.x |
container_title |
Letters in Applied Microbiology |
container_volume |
51 |
container_issue |
5 |
container_start_page |
564 |
op_container_end_page |
569 |
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1810475752069529600 |