Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification
Ocean acidification (OA) has posed formidable threats to marine calcifiers. In response to elevated CO2 levels, marine calcifiers have developed multiple strategies to survive, such as taking advantage of apoptosis, but its regulation mechanism remains largely unknown. Here, we used the Pacific oyst...
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Language: | English |
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ACADEMIC PRESS INC ELSEVIER SCIENCE
2021
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Online Access: | http://ir.qdio.ac.cn/handle/337002/170983 http://ir.qdio.ac.cn/handle/337002/170984 https://doi.org/10.1016/j.ecoenv.2021.112235 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/170984 2023-05-15T15:57:50+02:00 Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification Wang, Xiudan Li, Changmei Jia, Zhihao Xu, Tongxiao Wang, Yilin Sun, Mingzhu Han, Shuhui Wang, Xia Qiu, Limei 2021-07-01 http://ir.qdio.ac.cn/handle/337002/170983 http://ir.qdio.ac.cn/handle/337002/170984 https://doi.org/10.1016/j.ecoenv.2021.112235 英语 eng ACADEMIC PRESS INC ELSEVIER SCIENCE ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY http://ir.qdio.ac.cn/handle/337002/170983 http://ir.qdio.ac.cn/handle/337002/170984 doi:10.1016/j.ecoenv.2021.112235 Ocean acidification Crassostrea gigas Apoptosis CgBcl-XL Environmental Sciences & Ecology Toxicology Environmental Sciences 期刊论文 2021 ftchinacasciocas https://doi.org/10.1016/j.ecoenv.2021.112235 2022-06-27T05:43:52Z Ocean acidification (OA) has posed formidable threats to marine calcifiers. In response to elevated CO2 levels, marine calcifiers have developed multiple strategies to survive, such as taking advantage of apoptosis, but its regulation mechanism remains largely unknown. Here, we used the Pacific oyster Crassostrea gigas as model to understand the apoptotic responses and regulation mechanism at short- (7 d) to long-term (56 d) CO2 exposure (pH = 7.50). The apoptosis of hemocytes was significantly induced after short-term treatment (7-21 d) but was suppressed under long-term CO2 exposure (42-56 d). Similarly, caspase-3 and caspase-9 were also increased post short-term exposure and fell back to normal levels after long-term exposure. These data together indicated diverse regulation mechanisms of apoptosis through different exposure periods. Through analysis of the B-cell lymphoma 2 (Bcl-2) family mitochondrial apoptosis regulators, we showed that only CgBcl-XL's expression kept at high levels after 42- and 56-day CO2 exposure. CgBcl-XL shared sequence, and structural similarity with its mammalian counterpart, and knockdown of CgBcl-XL in hemocytes via RNA interference promoted apoptosis. The protein level of CgBcl-XL was significantly increased after long-term CO2 exposure (28-56 d), and its distribution in hemocytes became more concentrated and dense. Therefore, CgBcl-XL serves as an essential antiapoptotic protein for tipping the balance of cell apoptosis, which may play a key role in survival under longterm CO2 exposure. These results reveal a potential adaptation strategy of oysters towards OA and the variable environment changes through the modulation of apoptosis. Report Crassostrea gigas Ocean acidification Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Ecotoxicology and Environmental Safety 217 112235 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Ocean acidification Crassostrea gigas Apoptosis CgBcl-XL Environmental Sciences & Ecology Toxicology Environmental Sciences |
spellingShingle |
Ocean acidification Crassostrea gigas Apoptosis CgBcl-XL Environmental Sciences & Ecology Toxicology Environmental Sciences Wang, Xiudan Li, Changmei Jia, Zhihao Xu, Tongxiao Wang, Yilin Sun, Mingzhu Han, Shuhui Wang, Xia Qiu, Limei Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification |
topic_facet |
Ocean acidification Crassostrea gigas Apoptosis CgBcl-XL Environmental Sciences & Ecology Toxicology Environmental Sciences |
description |
Ocean acidification (OA) has posed formidable threats to marine calcifiers. In response to elevated CO2 levels, marine calcifiers have developed multiple strategies to survive, such as taking advantage of apoptosis, but its regulation mechanism remains largely unknown. Here, we used the Pacific oyster Crassostrea gigas as model to understand the apoptotic responses and regulation mechanism at short- (7 d) to long-term (56 d) CO2 exposure (pH = 7.50). The apoptosis of hemocytes was significantly induced after short-term treatment (7-21 d) but was suppressed under long-term CO2 exposure (42-56 d). Similarly, caspase-3 and caspase-9 were also increased post short-term exposure and fell back to normal levels after long-term exposure. These data together indicated diverse regulation mechanisms of apoptosis through different exposure periods. Through analysis of the B-cell lymphoma 2 (Bcl-2) family mitochondrial apoptosis regulators, we showed that only CgBcl-XL's expression kept at high levels after 42- and 56-day CO2 exposure. CgBcl-XL shared sequence, and structural similarity with its mammalian counterpart, and knockdown of CgBcl-XL in hemocytes via RNA interference promoted apoptosis. The protein level of CgBcl-XL was significantly increased after long-term CO2 exposure (28-56 d), and its distribution in hemocytes became more concentrated and dense. Therefore, CgBcl-XL serves as an essential antiapoptotic protein for tipping the balance of cell apoptosis, which may play a key role in survival under longterm CO2 exposure. These results reveal a potential adaptation strategy of oysters towards OA and the variable environment changes through the modulation of apoptosis. |
format |
Report |
author |
Wang, Xiudan Li, Changmei Jia, Zhihao Xu, Tongxiao Wang, Yilin Sun, Mingzhu Han, Shuhui Wang, Xia Qiu, Limei |
author_facet |
Wang, Xiudan Li, Changmei Jia, Zhihao Xu, Tongxiao Wang, Yilin Sun, Mingzhu Han, Shuhui Wang, Xia Qiu, Limei |
author_sort |
Wang, Xiudan |
title |
Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification |
title_short |
Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification |
title_full |
Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification |
title_fullStr |
Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification |
title_full_unstemmed |
Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification |
title_sort |
regulation of apoptosis by pacific oyster crassostrea gigas reveals acclimation strategy to co2 driven acidification |
publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
publishDate |
2021 |
url |
http://ir.qdio.ac.cn/handle/337002/170983 http://ir.qdio.ac.cn/handle/337002/170984 https://doi.org/10.1016/j.ecoenv.2021.112235 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_relation |
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY http://ir.qdio.ac.cn/handle/337002/170983 http://ir.qdio.ac.cn/handle/337002/170984 doi:10.1016/j.ecoenv.2021.112235 |
op_doi |
https://doi.org/10.1016/j.ecoenv.2021.112235 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
217 |
container_start_page |
112235 |
_version_ |
1766393538980872192 |