Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems
Nitrate (NO3-), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3- levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maxim...
Published in: | Ecotoxicology and Environmental Safety |
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Format: | Report |
Language: | English |
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ACADEMIC PRESS INC ELSEVIER SCIENCE
2021
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Online Access: | http://ir.qdio.ac.cn/handle/337002/169888 https://doi.org/10.1016/j.ecoenv.2020.111617 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/169888 2023-05-15T18:15:48+02:00 Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems Yu, Jiachen Wang, Yanfeng Xiao, Yongshuang Li, Xian Zhou, Li Wang, Yunong Du, Tengfei Ma, Xiaona Li, Jun 2021-01-15 http://ir.qdio.ac.cn/handle/337002/169888 https://doi.org/10.1016/j.ecoenv.2020.111617 英语 eng ACADEMIC PRESS INC ELSEVIER SCIENCE ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY http://ir.qdio.ac.cn/handle/337002/169888 doi:10.1016/j.ecoenv.2020.111617 Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity Environmental Sciences & Ecology Toxicology Environmental Sciences ZEBRAFISH DANIO-RERIO EARLY-LIFE STAGES RAINBOW-TROUT GENE-EXPRESSION THYROID-HORMONES BODY-COMPOSITION WATER-QUALITY IGF-I ONCORHYNCHUS-MYKISS OXIDATIVE STRESS 期刊论文 2021 ftchinacasciocas https://doi.org/10.1016/j.ecoenv.2020.111617 2022-06-27T05:43:29Z Nitrate (NO3-), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3- levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems (RAS). Fish were exposed to 0 mg/L (control, CK), 50 mg/L (low nitrate, LN), 200 mg/L (medium nitrate, MN), and 400 mg/L (high nitrate, HN) NO3-N for 60 d in experimental RAS. Cumulative survival (CS) was significantly decreased with increasing NO3- levels in LN, MN, and HN. The lowest CS was 35% in the HN group. Growth parameters, including absolute growth rate, specific growth rate, and feed conversion rate, were significantly different in HN compared with that in the CK. Histological survey of gills and liver revealed dose dependent histopathological damage induced by NO3- exposure and significant differences in glutamate pyruvate transaminase and glutamate oxalate transaminase in MN and HN compared with that in the CK. The hepatosomatic index in HN was significantly higher than that in the CK. Additionally, NO3- significantly increased bioaccumulation in plasma in LN, MN, and HN compared to that in the CK. Significant decreases in hemoglobin and increases in methemoglobin levels indicated reduced oxygen-carrying capacity in HN. Additionally, qRTPCR and enzyme-linked immunosorbent assay (ELISA) were developed to investigate key biomarkers involved in the GH/IGF-1, HPT, and HPI axes. Compared with that in the CK, the abundance of GH, GHRb, and IGF-1 was significantly lower in HN, whereas GHRa did not differ between treatments. The plasma T3 level significantly decreased in LN, MN, and HN and T4 significantly decreased in HN. The CRH, ACTH, and plasma cortisol levels were significantly upregulated in HN compared with that in the CK. We conclude that elevated NO3- exposure leads to growth retardation, impaired health status, and endocrine disorders in turbot and the NO3- level ... Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Ecotoxicology and Environmental Safety 208 111617 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity Environmental Sciences & Ecology Toxicology Environmental Sciences ZEBRAFISH DANIO-RERIO EARLY-LIFE STAGES RAINBOW-TROUT GENE-EXPRESSION THYROID-HORMONES BODY-COMPOSITION WATER-QUALITY IGF-I ONCORHYNCHUS-MYKISS OXIDATIVE STRESS |
spellingShingle |
Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity Environmental Sciences & Ecology Toxicology Environmental Sciences ZEBRAFISH DANIO-RERIO EARLY-LIFE STAGES RAINBOW-TROUT GENE-EXPRESSION THYROID-HORMONES BODY-COMPOSITION WATER-QUALITY IGF-I ONCORHYNCHUS-MYKISS OXIDATIVE STRESS Yu, Jiachen Wang, Yanfeng Xiao, Yongshuang Li, Xian Zhou, Li Wang, Yunong Du, Tengfei Ma, Xiaona Li, Jun Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
topic_facet |
Nitrate exposure Recirculating aquaculture systems (RAS) Juvenile turbot Aquaculture Toxicity Environmental Sciences & Ecology Toxicology Environmental Sciences ZEBRAFISH DANIO-RERIO EARLY-LIFE STAGES RAINBOW-TROUT GENE-EXPRESSION THYROID-HORMONES BODY-COMPOSITION WATER-QUALITY IGF-I ONCORHYNCHUS-MYKISS OXIDATIVE STRESS |
description |
Nitrate (NO3-), a potential toxic nitrogenous compound to aquatic animals, is distributed in aquatic ecosystems worldwide. The aim of this study was to investigate the effects of different NO3- levels on growth performance, health status, and endocrine function of juvenile turbot (Scophthalmus maximus) in recirculating aquaculture systems (RAS). Fish were exposed to 0 mg/L (control, CK), 50 mg/L (low nitrate, LN), 200 mg/L (medium nitrate, MN), and 400 mg/L (high nitrate, HN) NO3-N for 60 d in experimental RAS. Cumulative survival (CS) was significantly decreased with increasing NO3- levels in LN, MN, and HN. The lowest CS was 35% in the HN group. Growth parameters, including absolute growth rate, specific growth rate, and feed conversion rate, were significantly different in HN compared with that in the CK. Histological survey of gills and liver revealed dose dependent histopathological damage induced by NO3- exposure and significant differences in glutamate pyruvate transaminase and glutamate oxalate transaminase in MN and HN compared with that in the CK. The hepatosomatic index in HN was significantly higher than that in the CK. Additionally, NO3- significantly increased bioaccumulation in plasma in LN, MN, and HN compared to that in the CK. Significant decreases in hemoglobin and increases in methemoglobin levels indicated reduced oxygen-carrying capacity in HN. Additionally, qRTPCR and enzyme-linked immunosorbent assay (ELISA) were developed to investigate key biomarkers involved in the GH/IGF-1, HPT, and HPI axes. Compared with that in the CK, the abundance of GH, GHRb, and IGF-1 was significantly lower in HN, whereas GHRa did not differ between treatments. The plasma T3 level significantly decreased in LN, MN, and HN and T4 significantly decreased in HN. The CRH, ACTH, and plasma cortisol levels were significantly upregulated in HN compared with that in the CK. We conclude that elevated NO3- exposure leads to growth retardation, impaired health status, and endocrine disorders in turbot and the NO3- level ... |
format |
Report |
author |
Yu, Jiachen Wang, Yanfeng Xiao, Yongshuang Li, Xian Zhou, Li Wang, Yunong Du, Tengfei Ma, Xiaona Li, Jun |
author_facet |
Yu, Jiachen Wang, Yanfeng Xiao, Yongshuang Li, Xian Zhou, Li Wang, Yunong Du, Tengfei Ma, Xiaona Li, Jun |
author_sort |
Yu, Jiachen |
title |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_short |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_full |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_fullStr |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_full_unstemmed |
Investigating the effect of nitrate on juvenile turbot (Scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
title_sort |
investigating the effect of nitrate on juvenile turbot (scophthalmus maximus) growth performance, health status, and endocrine function in marine recirculation aquaculture systems |
publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
publishDate |
2021 |
url |
http://ir.qdio.ac.cn/handle/337002/169888 https://doi.org/10.1016/j.ecoenv.2020.111617 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY http://ir.qdio.ac.cn/handle/337002/169888 doi:10.1016/j.ecoenv.2020.111617 |
op_doi |
https://doi.org/10.1016/j.ecoenv.2020.111617 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
208 |
container_start_page |
111617 |
_version_ |
1766189022176083968 |