Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas
DM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs). In the present study, a novel...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Report |
Language: | English |
Published: |
FRONTIERS MEDIA SA
2021
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/169757 https://doi.org/10.3389/fimmu.2020.603270 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/169757 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/169757 2023-05-15T15:57:55+02:00 Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas Jia, Zhihao Jiang, Shuai Wang, Mengqiang Wang, Xiudan Liu, Yu Lv, Zhao Song, Xiaorui Li, Yiqun Wang, Lingling Song, Linsheng 2021-02-12 http://ir.qdio.ac.cn/handle/337002/169757 https://doi.org/10.3389/fimmu.2020.603270 英语 eng FRONTIERS MEDIA SA FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/169757 doi:10.3389/fimmu.2020.603270 Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes Immunology 期刊论文 2021 ftchinacasciocas https://doi.org/10.3389/fimmu.2020.603270 2022-06-27T05:43:26Z DM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs). In the present study, a novel member of DM9 domain containing protein (designated as CgDM9CP-4) was identified from C. gigas. CgDM9CP-4, about 16 kDa with only two tandem DM9 domains, was highly enriched in hemocytes and gill. The transcripts level of CgDM9CP-4 in circulating hemocytes were decreased after LPS, PGN and Vibrio splendidus stimulations. The recombinant protein of CgDM9CP-4 (rCgDM9CP-4) displayed a broad binding spectrum towards various pathogen-associated molecular patterns (PAMPs) (LPS, PGN, beta-glucan and Mannose) and microorganisms (Staphylococcus aureus, Micrococcus luteus, V. splendidus, V. anguillarum, Escherichia coli, Pichia pastoris and Yarrowia lipolytica). CgDM9CP-4 was mostly expressed in gill and some of the hemocytes. Flow cytometry analysis demonstrated that the CgDM9CP-4-positive hemocytes accounted for 7.3% of the total hemocytes, and they were small in size and less in granularity. CgDM9CP-4 was highly expressed in non-phagocytes (similar to 82% of total hemocytes). The reactive oxygen species (ROS) and the expression levels of cytokines in CgDM9CP-4-positive hemocytes were much lower than that in CgDM9CP-4-negative hemocytes. The mRNA expression level of CgDM9CP-4 in hemocytes was decreased after RNAi of hematopoietic-related factors (CgGATA, CgRunt, CgSCL, and CgNotch). In addition, CgDM9CP-4-positive cells were found to be much more abundant in hemocytes from gill than that from hemolymph, with most of them located in the gill filament. All these results suggested that CgDM9CP-4 was a novel member of PRR that expressed in undifferentiated pro-hemocytes to mediate immune recognition of pathogens. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Frontiers in Immunology 11 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes Immunology |
spellingShingle |
Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes Immunology Jia, Zhihao Jiang, Shuai Wang, Mengqiang Wang, Xiudan Liu, Yu Lv, Zhao Song, Xiaorui Li, Yiqun Wang, Lingling Song, Linsheng Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas DM9 domain pattern recognition innate immune response pro-hemocytes Immunology |
description |
DM9 refers to an uncharacterized protein domain that is originally discovered in Drosophila melanogaster. Two proteins with DM9 repeats have been recently identified from Pacific oyster Crassostrea gigas as mannose-specific binding pattern-recognition receptors (PRRs). In the present study, a novel member of DM9 domain containing protein (designated as CgDM9CP-4) was identified from C. gigas. CgDM9CP-4, about 16 kDa with only two tandem DM9 domains, was highly enriched in hemocytes and gill. The transcripts level of CgDM9CP-4 in circulating hemocytes were decreased after LPS, PGN and Vibrio splendidus stimulations. The recombinant protein of CgDM9CP-4 (rCgDM9CP-4) displayed a broad binding spectrum towards various pathogen-associated molecular patterns (PAMPs) (LPS, PGN, beta-glucan and Mannose) and microorganisms (Staphylococcus aureus, Micrococcus luteus, V. splendidus, V. anguillarum, Escherichia coli, Pichia pastoris and Yarrowia lipolytica). CgDM9CP-4 was mostly expressed in gill and some of the hemocytes. Flow cytometry analysis demonstrated that the CgDM9CP-4-positive hemocytes accounted for 7.3% of the total hemocytes, and they were small in size and less in granularity. CgDM9CP-4 was highly expressed in non-phagocytes (similar to 82% of total hemocytes). The reactive oxygen species (ROS) and the expression levels of cytokines in CgDM9CP-4-positive hemocytes were much lower than that in CgDM9CP-4-negative hemocytes. The mRNA expression level of CgDM9CP-4 in hemocytes was decreased after RNAi of hematopoietic-related factors (CgGATA, CgRunt, CgSCL, and CgNotch). In addition, CgDM9CP-4-positive cells were found to be much more abundant in hemocytes from gill than that from hemolymph, with most of them located in the gill filament. All these results suggested that CgDM9CP-4 was a novel member of PRR that expressed in undifferentiated pro-hemocytes to mediate immune recognition of pathogens. |
format |
Report |
author |
Jia, Zhihao Jiang, Shuai Wang, Mengqiang Wang, Xiudan Liu, Yu Lv, Zhao Song, Xiaorui Li, Yiqun Wang, Lingling Song, Linsheng |
author_facet |
Jia, Zhihao Jiang, Shuai Wang, Mengqiang Wang, Xiudan Liu, Yu Lv, Zhao Song, Xiaorui Li, Yiqun Wang, Lingling Song, Linsheng |
author_sort |
Jia, Zhihao |
title |
Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas |
title_short |
Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas |
title_full |
Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas |
title_fullStr |
Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Identification of a Novel Pattern Recognition Receptor DM9 Domain Containing Protein 4 as a Marker for Pro-Hemocyte of Pacific Oyster Crassostrea gigas |
title_sort |
identification of a novel pattern recognition receptor dm9 domain containing protein 4 as a marker for pro-hemocyte of pacific oyster crassostrea gigas |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2021 |
url |
http://ir.qdio.ac.cn/handle/337002/169757 https://doi.org/10.3389/fimmu.2020.603270 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/169757 doi:10.3389/fimmu.2020.603270 |
op_doi |
https://doi.org/10.3389/fimmu.2020.603270 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
_version_ |
1766393629764485120 |