Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification
There is increasing evidence that ocean acidification (OA) has a significant impact on marine organisms. How ever, the ability of most marine organisms to acclimate to OA and the underlying mechanisms are still not well understood. In the present study, whole transcriptome analysis was performed to...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/168793 2023-05-15T15:57:46+02:00 Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification Wang, Xiudan Wang, Mengqiang Wang, Weilin Liu, Zhaoqun Xu, Jiachao Jia, Zhihao Chen, Hao Qiu, Limei Lv, Zhao Wang, Lingling Song, Linsheng 2020-11-01 http://ir.qdio.ac.cn/handle/337002/168792 http://ir.qdio.ac.cn/handle/337002/168793 https://doi.org/10.1016/j.scitotenv.2020.140177 英语 eng ELSEVIER SCIENCE OF THE TOTAL ENVIRONMENT http://ir.qdio.ac.cn/handle/337002/168792 http://ir.qdio.ac.cn/handle/337002/168793 doi:10.1016/j.scitotenv.2020.140177 Transcriptome Crassostrea gigas Calcium signal CO2-driven acidification Environmental Sciences & Ecology Environmental Sciences 期刊论文 2020 ftchinacasciocas https://doi.org/10.1016/j.scitotenv.2020.140177 2022-06-27T05:43:13Z There is increasing evidence that ocean acidification (OA) has a significant impact on marine organisms. How ever, the ability of most marine organisms to acclimate to OA and the underlying mechanisms are still not well understood. In the present study, whole transcriptome analysis was performed to compare the impacts of short(7 days, named as short group) and long(60 days, named as long group) term CO2 exposure (pH 7.50) on Pacific oyster Crassostrea gigas. The responses of C. gigas to shortand long-term CO2 exposure shared common mechanisms in metabolism, membrane-associated transportation and binding processes. Long-term CO2 exposure induced significant expression of genes involved in DNA or RNA binding, indicating the activated transcription after long-term CO2 exposure. Oysters in the short-term group underwent significant intracellular calcium variation and oxidative stress. In contrast, the intracellular calcium, ROS level in hemocytes and H2O2 in serum recovered to normal levels after long-term CO2 exposure, suggesting the compensation of physiological status and mutual interplay between calcium and oxidative level. The compensation was supported by the upregulation of a series of calcium binding proteins (CBPs) and calmodulins (CaMs) related signal pathway. The results provided valuable information to understand the molecular mechanism underlying the responses of Pacific oyster to the acidified ocean and might have implications for predicting the possible effects of global climate changes on oyster aquaculture. (c) 2020 Elsevier B.V. All rights reserved. Report Crassostrea gigas Ocean acidification Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Science of The Total Environment 741 140177 |
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Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Transcriptome Crassostrea gigas Calcium signal CO2-driven acidification Environmental Sciences & Ecology Environmental Sciences |
spellingShingle |
Transcriptome Crassostrea gigas Calcium signal CO2-driven acidification Environmental Sciences & Ecology Environmental Sciences Wang, Xiudan Wang, Mengqiang Wang, Weilin Liu, Zhaoqun Xu, Jiachao Jia, Zhihao Chen, Hao Qiu, Limei Lv, Zhao Wang, Lingling Song, Linsheng Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification |
topic_facet |
Transcriptome Crassostrea gigas Calcium signal CO2-driven acidification Environmental Sciences & Ecology Environmental Sciences |
description |
There is increasing evidence that ocean acidification (OA) has a significant impact on marine organisms. How ever, the ability of most marine organisms to acclimate to OA and the underlying mechanisms are still not well understood. In the present study, whole transcriptome analysis was performed to compare the impacts of short(7 days, named as short group) and long(60 days, named as long group) term CO2 exposure (pH 7.50) on Pacific oyster Crassostrea gigas. The responses of C. gigas to shortand long-term CO2 exposure shared common mechanisms in metabolism, membrane-associated transportation and binding processes. Long-term CO2 exposure induced significant expression of genes involved in DNA or RNA binding, indicating the activated transcription after long-term CO2 exposure. Oysters in the short-term group underwent significant intracellular calcium variation and oxidative stress. In contrast, the intracellular calcium, ROS level in hemocytes and H2O2 in serum recovered to normal levels after long-term CO2 exposure, suggesting the compensation of physiological status and mutual interplay between calcium and oxidative level. The compensation was supported by the upregulation of a series of calcium binding proteins (CBPs) and calmodulins (CaMs) related signal pathway. The results provided valuable information to understand the molecular mechanism underlying the responses of Pacific oyster to the acidified ocean and might have implications for predicting the possible effects of global climate changes on oyster aquaculture. (c) 2020 Elsevier B.V. All rights reserved. |
format |
Report |
author |
Wang, Xiudan Wang, Mengqiang Wang, Weilin Liu, Zhaoqun Xu, Jiachao Jia, Zhihao Chen, Hao Qiu, Limei Lv, Zhao Wang, Lingling Song, Linsheng |
author_facet |
Wang, Xiudan Wang, Mengqiang Wang, Weilin Liu, Zhaoqun Xu, Jiachao Jia, Zhihao Chen, Hao Qiu, Limei Lv, Zhao Wang, Lingling Song, Linsheng |
author_sort |
Wang, Xiudan |
title |
Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification |
title_short |
Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification |
title_full |
Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification |
title_fullStr |
Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification |
title_full_unstemmed |
Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification |
title_sort |
transcriptional changes of pacific oyster crassostrea gigas reveal essential role of calcium signal pathway in response to co2-driven acidification |
publisher |
ELSEVIER |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/168792 http://ir.qdio.ac.cn/handle/337002/168793 https://doi.org/10.1016/j.scitotenv.2020.140177 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_relation |
SCIENCE OF THE TOTAL ENVIRONMENT http://ir.qdio.ac.cn/handle/337002/168792 http://ir.qdio.ac.cn/handle/337002/168793 doi:10.1016/j.scitotenv.2020.140177 |
op_doi |
https://doi.org/10.1016/j.scitotenv.2020.140177 |
container_title |
Science of The Total Environment |
container_volume |
741 |
container_start_page |
140177 |
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1766393467454357504 |