Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar
Triploid Atlantic salmon Salmo salar is commercially available and ideal means of achieving sterility in fish. The expression patterns of triploid parr and smolt were described before. However, little is known about the gene expression and fatty acid composition of triploid alevins at endogenous nut...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/168769 2023-05-15T15:30:15+02:00 Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar Wu, Zhihao Xu, Shihong Wang, Lijuan Wu, Qiaowan Li, Jun Liu, Hao You, Feng 2020-10-01 http://ir.qdio.ac.cn/handle/337002/168768 http://ir.qdio.ac.cn/handle/337002/168769 https://doi.org/10.1016/j.theriogenology.2020.06.007 英语 eng ELSEVIER SCIENCE INC THERIOGENOLOGY http://ir.qdio.ac.cn/handle/337002/168768 http://ir.qdio.ac.cn/handle/337002/168769 doi:10.1016/j.theriogenology.2020.06.007 Atlantic salmon Salmo salar Triploid Gene expression Fatty acid composition Reproductive Biology Veterinary Sciences 期刊论文 2020 ftchinacasciocas https://doi.org/10.1016/j.theriogenology.2020.06.007 2022-06-27T05:43:13Z Triploid Atlantic salmon Salmo salar is commercially available and ideal means of achieving sterility in fish. The expression patterns of triploid parr and smolt were described before. However, little is known about the gene expression and fatty acid composition of triploid alevins at endogenous nutritional stage, which is an important period for their survival and development. In this study, the development of the diploid and triploid Atlantic salmon embryos and hatched alevins before feeding was compared, and the results showed that there was no obvious morphological difference between them. And then, the transcription profiles of the triploid and diploid alevins on 3, 23 and 33 days post hatching (dph) were investigated by using RNA-seq after the yolk sacs were removed. There were more differentially expressed genes (DEGs) on 3 dph (2925) than those on 23 (589) and 33 dph (606). Compared with the diploid groups, the up-regulated genes in the triploid groups at the three sampling times were 1189, 339 and 340, respectively, while 1736, 250 and 266 genes were down-regulated. There were 2088 and 5215 DEGs on 23 and 33 dph compared with on 3 dph in the diploid groups, while 6533 and 9340 DEGS on 23 and 33 dph compared with 3 dph in the triploid groups. The functional enrichment analysis showed that the DEGs were mainly enriched to digestion and metabolism function, especially on 33 dph when the larvae were about to feeding. Meanwhile, 15 out of 22 kinds of fatty acids of alevins on 33 dph showed significant differences (P < 0.05), in which all the fatty acids contents in the triploid were lower. The results indicated the specific nutritional requirement in the triploid was represented as early as yolk sac stage. The present study gave an important vision in triploid Atlantic salmon breeding. (C) 2020 Elsevier Inc. All rights reserved. Report Atlantic salmon Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Theriogenology 155 49 59 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Atlantic salmon Salmo salar Triploid Gene expression Fatty acid composition Reproductive Biology Veterinary Sciences |
spellingShingle |
Atlantic salmon Salmo salar Triploid Gene expression Fatty acid composition Reproductive Biology Veterinary Sciences Wu, Zhihao Xu, Shihong Wang, Lijuan Wu, Qiaowan Li, Jun Liu, Hao You, Feng Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar |
topic_facet |
Atlantic salmon Salmo salar Triploid Gene expression Fatty acid composition Reproductive Biology Veterinary Sciences |
description |
Triploid Atlantic salmon Salmo salar is commercially available and ideal means of achieving sterility in fish. The expression patterns of triploid parr and smolt were described before. However, little is known about the gene expression and fatty acid composition of triploid alevins at endogenous nutritional stage, which is an important period for their survival and development. In this study, the development of the diploid and triploid Atlantic salmon embryos and hatched alevins before feeding was compared, and the results showed that there was no obvious morphological difference between them. And then, the transcription profiles of the triploid and diploid alevins on 3, 23 and 33 days post hatching (dph) were investigated by using RNA-seq after the yolk sacs were removed. There were more differentially expressed genes (DEGs) on 3 dph (2925) than those on 23 (589) and 33 dph (606). Compared with the diploid groups, the up-regulated genes in the triploid groups at the three sampling times were 1189, 339 and 340, respectively, while 1736, 250 and 266 genes were down-regulated. There were 2088 and 5215 DEGs on 23 and 33 dph compared with on 3 dph in the diploid groups, while 6533 and 9340 DEGS on 23 and 33 dph compared with 3 dph in the triploid groups. The functional enrichment analysis showed that the DEGs were mainly enriched to digestion and metabolism function, especially on 33 dph when the larvae were about to feeding. Meanwhile, 15 out of 22 kinds of fatty acids of alevins on 33 dph showed significant differences (P < 0.05), in which all the fatty acids contents in the triploid were lower. The results indicated the specific nutritional requirement in the triploid was represented as early as yolk sac stage. The present study gave an important vision in triploid Atlantic salmon breeding. (C) 2020 Elsevier Inc. All rights reserved. |
format |
Report |
author |
Wu, Zhihao Xu, Shihong Wang, Lijuan Wu, Qiaowan Li, Jun Liu, Hao You, Feng |
author_facet |
Wu, Zhihao Xu, Shihong Wang, Lijuan Wu, Qiaowan Li, Jun Liu, Hao You, Feng |
author_sort |
Wu, Zhihao |
title |
Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar |
title_short |
Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar |
title_full |
Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar |
title_fullStr |
Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar |
title_full_unstemmed |
Transcription profiles and fatty acid composition of the artificial induced triploid alevin in Atlantic salmon Salmo salar |
title_sort |
transcription profiles and fatty acid composition of the artificial induced triploid alevin in atlantic salmon salmo salar |
publisher |
ELSEVIER SCIENCE INC |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/168768 http://ir.qdio.ac.cn/handle/337002/168769 https://doi.org/10.1016/j.theriogenology.2020.06.007 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
THERIOGENOLOGY http://ir.qdio.ac.cn/handle/337002/168768 http://ir.qdio.ac.cn/handle/337002/168769 doi:10.1016/j.theriogenology.2020.06.007 |
op_doi |
https://doi.org/10.1016/j.theriogenology.2020.06.007 |
container_title |
Theriogenology |
container_volume |
155 |
container_start_page |
49 |
op_container_end_page |
59 |
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1766360695547363328 |