A combined analysis of transcriptomics and proteomics of a novel Antarctic Salpa sp. and its potential toxin screenings

Background: We successfully captured a kind of gelatinous organism DA-6 from Antarctic water, extracted its total RNAs and proteins, and performed species identification through a combination of transcriptomics and proteomics in this study. Methods: The gelatinous organism DA-6 was captured 200 m un...

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Bibliographic Details
Published in:International Journal of Biological Macromolecules
Main Authors: Ye, Ruiwei, Liang, Hongyu, Chen, Ting, Zhang, Jie, Yang, Fengling, Chen, Xintong, Li, Xiaoya, Gao, Songyu, Yang, Ying, Wang, Yongqiang, Tao, Feng, Zhang, Jing, Xiao, Liang
Format: Report
Language:English
Published: ELSEVIER 2020
Subjects:
Antarctic
Salpa
Transcriptome
Proteome
Toxin
Biochemistry & Molecular Biology
Chemistry
Polymer Science
Applied
IDENTIFICATION
TUNICATE
GENE
Antarc*
Antarctica
Online Access:http://ir.qdio.ac.cn/handle/337002/168473
https://doi.org/10.1016/j.ijbiomac.2020.05.144
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Summary:Background: We successfully captured a kind of gelatinous organism DA-6 from Antarctic water, extracted its total RNAs and proteins, and performed species identification through a combination of transcriptomics and proteomics in this study. Methods: The gelatinous organism DA-6 was captured 200 m underwater in Antarctica. Total RNA was extracted to construct the transcriptome and the proteins were identified by LC-MS/MS. Results: DA-6 was identified as an Antarctic Salpa sp. through morphological examination and MT-CO1 phylogenetic analysis. A total of 47,183 unigenes were harvested through transcriptome. We also successfully annotated 11,954 (25.34%),10,006 (22.21%), 4469 (9.47%) and 4901 (9.71%) unigenes with NR, SwissProt, GO and KEGG databases, respectively. In the proteomic analysis, a total of 4680 peptides and 1280 proteins were harvested using the transcriptome as the reference database. A number of both 549 (31.98%) proteins reannotated against the GO and KEGG databases. Moreover, a number of 5 toxic proteins matched from the 89 toxin-related unigenes were successfully screened, including 2 metalloproteinases, 1 serine protease, 1 serine protease inhibitor and 1 aflatoxin. Conclusion: Our study is the first to identify an Antarctic Salpa sp. according to the combination of de novo transcriptomics and proteomics, which can further be served as a public database for the identification of potential polar Salpa-derived lead compounds. In addition to morphology and CO1, the combined analysis of transcriptome and proteome can also be used as a value method for new species identify e.g. Salpa sp. (C) 2020 Elsevier B.V. All rights reserved.

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