A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oysterCrassostrea gigasis, however, yet to be discovered. This w...
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Online Access: | http://ir.qdio.ac.cn/handle/337002/168180 http://ir.qdio.ac.cn/handle/337002/168181 https://doi.org/10.3389/fimmu.2020.01379 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/168181 2023-05-15T15:58:47+02:00 A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas Liu, Conghui Yang, Chuanyan Wang, Mengqiang Jiang, Shuai Yi, Qilin Wang, Weilin Wang, Lingling Song, Linsheng 2020-07-22 http://ir.qdio.ac.cn/handle/337002/168180 http://ir.qdio.ac.cn/handle/337002/168181 https://doi.org/10.3389/fimmu.2020.01379 英语 eng FRONTIERS MEDIA SA FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/168180 http://ir.qdio.ac.cn/handle/337002/168181 doi:10.3389/fimmu.2020.01379 tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunology 期刊论文 2020 ftchinacasciocas https://doi.org/10.3389/fimmu.2020.01379 2022-06-27T05:43:01Z Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oysterCrassostrea gigasis, however, yet to be discovered. This work makes contributions by identifyingCgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence ofCgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p< 0.01) upregulated after the injection ofVibrio splendidus.CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to theYarrowia lipolytica-containing phagosomes after the stimulation ofY. lipolytica. The recombinantCgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes towardV. splendidusandY. lipolyticais significantly inhibited (p< 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests thatCgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing inC. gigas. Report Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Frontiers in Immunology 11 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunology |
spellingShingle |
tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunology Liu, Conghui Yang, Chuanyan Wang, Mengqiang Jiang, Shuai Yi, Qilin Wang, Weilin Wang, Lingling Song, Linsheng A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas |
topic_facet |
tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas Immunology |
description |
Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oysterCrassostrea gigasis, however, yet to be discovered. This work makes contributions by identifyingCgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence ofCgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p< 0.01) upregulated after the injection ofVibrio splendidus.CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to theYarrowia lipolytica-containing phagosomes after the stimulation ofY. lipolytica. The recombinantCgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes towardV. splendidusandY. lipolyticais significantly inhibited (p< 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests thatCgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing inC. gigas. |
format |
Report |
author |
Liu, Conghui Yang, Chuanyan Wang, Mengqiang Jiang, Shuai Yi, Qilin Wang, Weilin Wang, Lingling Song, Linsheng |
author_facet |
Liu, Conghui Yang, Chuanyan Wang, Mengqiang Jiang, Shuai Yi, Qilin Wang, Weilin Wang, Lingling Song, Linsheng |
author_sort |
Liu, Conghui |
title |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas |
title_short |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas |
title_full |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas |
title_fullStr |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas |
title_full_unstemmed |
A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas |
title_sort |
cd63 homolog specially recruited to the fungi-contained phagosomes is involved in the cellular immune response of oystercrassostrea gigas |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/168180 http://ir.qdio.ac.cn/handle/337002/168181 https://doi.org/10.3389/fimmu.2020.01379 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/168180 http://ir.qdio.ac.cn/handle/337002/168181 doi:10.3389/fimmu.2020.01379 |
op_doi |
https://doi.org/10.3389/fimmu.2020.01379 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
_version_ |
1766394548945158144 |