CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)
Ostreid herpesvirus-1 microvariant (OsHV-1 mu Var) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and...
Published in: | Frontiers in Immunology |
---|---|
Main Authors: | , , , , , |
Format: | Report |
Language: | English |
Published: |
FRONTIERS MEDIA SA
2020
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/168129 http://ir.qdio.ac.cn/handle/337002/168130 https://doi.org/10.3389/fimmu.2020.01247 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/168130 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/168130 2023-05-15T15:58:20+02:00 CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) Tang, Xueying Huang, Baoyu Lin, Siheng Wang, Wei Zhang, Guofan Li, Li 2020-07-14 http://ir.qdio.ac.cn/handle/337002/168129 http://ir.qdio.ac.cn/handle/337002/168130 https://doi.org/10.3389/fimmu.2020.01247 英语 eng FRONTIERS MEDIA SA FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/168129 http://ir.qdio.ac.cn/handle/337002/168130 doi:10.3389/fimmu.2020.01247 innate immunity OsHV-1 mu Var Crassostrea gigas TLR MyD88 Immunology 期刊论文 2020 ftchinacasciocas https://doi.org/10.3389/fimmu.2020.01247 2022-06-27T05:42:58Z Ostreid herpesvirus-1 microvariant (OsHV-1 mu Var) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and functional divergence of innate immune genes in the oyster have been studied, but the contribution of molecular mechanisms underlying innate immunity remains to be defined. Here, we identified the interacting proteins associated withCrassostrea gigasToll-like receptors (CgTLR) using a yeast two-hybrid (Y2H) screening system. A total of eight proteins were identified that could interact with CgTLR. Three of these appeared at least four times in the screening and were related to MyD88. Two genes encoding these MyD88-like proteins, CgMyD88-1 and CgMyD88-2, possessed typical death and TIR domains. The third gene encoding an MyD88-like protein possessed only a TIR domain, and we named it CgMyD88s. CgMyD88s interacted only with CgTLR, but not CgMyD88-1 or CgMyD88-2. Both CgMyD88-1 and CgMyD88-2 mRNAs were upregulated after OsHV-1 mu Var infection, whereas the expression of CgMyD88s decreased. When overexpressed in HEK293T cells, CgMyD88-1 and CgMyD88-2 activated an NF-kappa B reporter, whereas CgMyD88s impaired activation induced by CgMyD88-1 or CgMyD88-2. Intriguingly, the silencing of CgMyD88s using double-stranded RNA (dsRNA)-mediated RNA interference increased the expression of CgMyD88-1 and CgMyD88-2. Taken together, our results revealed that CgMyD88-1, CgMyD88-2, and CgMyD88s may all participate in the TLR-mediated innate immune pathway and that CgMyD88s served as a plug to avoid oysters from excessive inflammatory response during OsHV-1 mu Var infections. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Frontiers in Immunology 11 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
innate immunity OsHV-1 mu Var Crassostrea gigas TLR MyD88 Immunology |
spellingShingle |
innate immunity OsHV-1 mu Var Crassostrea gigas TLR MyD88 Immunology Tang, Xueying Huang, Baoyu Lin, Siheng Wang, Wei Zhang, Guofan Li, Li CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
topic_facet |
innate immunity OsHV-1 mu Var Crassostrea gigas TLR MyD88 Immunology |
description |
Ostreid herpesvirus-1 microvariant (OsHV-1 mu Var) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and functional divergence of innate immune genes in the oyster have been studied, but the contribution of molecular mechanisms underlying innate immunity remains to be defined. Here, we identified the interacting proteins associated withCrassostrea gigasToll-like receptors (CgTLR) using a yeast two-hybrid (Y2H) screening system. A total of eight proteins were identified that could interact with CgTLR. Three of these appeared at least four times in the screening and were related to MyD88. Two genes encoding these MyD88-like proteins, CgMyD88-1 and CgMyD88-2, possessed typical death and TIR domains. The third gene encoding an MyD88-like protein possessed only a TIR domain, and we named it CgMyD88s. CgMyD88s interacted only with CgTLR, but not CgMyD88-1 or CgMyD88-2. Both CgMyD88-1 and CgMyD88-2 mRNAs were upregulated after OsHV-1 mu Var infection, whereas the expression of CgMyD88s decreased. When overexpressed in HEK293T cells, CgMyD88-1 and CgMyD88-2 activated an NF-kappa B reporter, whereas CgMyD88s impaired activation induced by CgMyD88-1 or CgMyD88-2. Intriguingly, the silencing of CgMyD88s using double-stranded RNA (dsRNA)-mediated RNA interference increased the expression of CgMyD88-1 and CgMyD88-2. Taken together, our results revealed that CgMyD88-1, CgMyD88-2, and CgMyD88s may all participate in the TLR-mediated innate immune pathway and that CgMyD88s served as a plug to avoid oysters from excessive inflammatory response during OsHV-1 mu Var infections. |
format |
Report |
author |
Tang, Xueying Huang, Baoyu Lin, Siheng Wang, Wei Zhang, Guofan Li, Li |
author_facet |
Tang, Xueying Huang, Baoyu Lin, Siheng Wang, Wei Zhang, Guofan Li, Li |
author_sort |
Tang, Xueying |
title |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_short |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_full |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_fullStr |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_full_unstemmed |
CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas) |
title_sort |
cgmyd88s serves as an innate immune system plug during ostreid herpesvirus 1 infection in the pacific oyster (crassostrea gigas) |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/168129 http://ir.qdio.ac.cn/handle/337002/168130 https://doi.org/10.3389/fimmu.2020.01247 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FRONTIERS IN IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/168129 http://ir.qdio.ac.cn/handle/337002/168130 doi:10.3389/fimmu.2020.01247 |
op_doi |
https://doi.org/10.3389/fimmu.2020.01247 |
container_title |
Frontiers in Immunology |
container_volume |
11 |
_version_ |
1766394065604050944 |