The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas

• Published in: Frontiers in Marine Science
• Main Authors: Ding, Fangfang, Li, Ao, Cong, Rihao, Wang, Xinxing, Wang, Wei, Que, Huayong, Zhang, Guofan, Li, Li
• Format: Report
• Language: English
• Published: FRONTIERS MEDIA SA 2020
• Subjects: Pacific oyster; artificial selection; thermotolerance; genetic structure; physiological traits; Environmental Sciences & Ecology; Marine & Freshwater Biology; Environmental Sciences; Pacific; Crassostrea gigas
• Online Access: http://ir.qdio.ac.cn/handle/337002/167948; http://ir.qdio.ac.cn/handle/337002/167949; https://doi.org/10.3389/fmars.2020.00399

Investigating responses of organisms to stressful or new environments with selection pressure is one of the crucial problems in evolutionary biology, and it is of importance to understand the phenotypic and molecular mechanism underlying thermal tolerance under the context of the climate change. The Pacific oyster, Crassostrea gigas, inhabiting the environment with high variation in temperature, is a worldwide aquaculture species. However, summer mortality relevant to the high temperature is one of the problems challenging the oyster industry. An artificial selective breeding program was initiated to select for the thermal tolerance of oysters in an attempt to increase the summer survival rates since 2017 in our study. Parents of thermotolerance oyster selection is are based on acute thermal tolerance under controlled heat stress to strengthen the selection intensity. Furthermore, the phenotypic and the genotypic response to extreme high temperature were investigated based on the comparison of the F-1 progeny of the selected and natural populations in growth, physiology, mortality rate post heat stress, genetic structure, and gene expression. The parameter of growth showed no significant change between the selected and natural populations for the 6-month-old oysters. The selected population exhibited a higher survival rate after exposure to heat stress in the laboratory, which is in line with result of the filed experience that summer mortality of selected population was significantly lower than that of natural population. Further, the respiration rate of the selected population increased at 38 degrees C, while it increased at 35 degrees C in the natural population. Simultaneously, metabolism-related enzymes (PK, SOD) showed higher activity levels in the selected population. Furthermore, phylogenetic analysis, population structure, and principal component analysis (PCA) suggested that the selected and natural populations exhibited genetic divergence, with eight genes (IF4A2, IF6, EIF3A, MANBA, DDX43, RECS, CAT2, ...