The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas
Investigating responses of organisms to stressful or new environments with selection pressure is one of the crucial problems in evolutionary biology, and it is of importance to understand the phenotypic and molecular mechanism underlying thermal tolerance under the context of the climate change. The...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/167947 2023-05-15T15:58:32+02:00 The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas Ding, Fangfang Li, Ao Cong, Rihao Wang, Xinxing Wang, Wei Que, Huayong Zhang, Guofan Li, Li 2020-06-30 http://ir.qdio.ac.cn/handle/337002/167947 https://doi.org/10.3389/fmars.2020.00399 英语 eng FRONTIERS MEDIA SA FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/167947 doi:10.3389/fmars.2020.00399 Pacific oyster artificial selection thermotolerance genetic structure physiological traits Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences SUMMER MORTALITY CLIMATE-CHANGE OXYGEN-CONSUMPTION LOCAL ADAPTATION STRESS LIMITATION RESISTANCE SELECTION INCREASE THUNBERG 期刊论文 2020 ftchinacasciocas https://doi.org/10.3389/fmars.2020.00399 2022-06-27T05:42:55Z Investigating responses of organisms to stressful or new environments with selection pressure is one of the crucial problems in evolutionary biology, and it is of importance to understand the phenotypic and molecular mechanism underlying thermal tolerance under the context of the climate change. The Pacific oyster, Crassostrea gigas, inhabiting the environment with high variation in temperature, is a worldwide aquaculture species. However, summer mortality relevant to the high temperature is one of the problems challenging the oyster industry. An artificial selective breeding program was initiated to select for the thermal tolerance of oysters in an attempt to increase the summer survival rates since 2017 in our study. Parents of thermotolerance oyster selection is are based on acute thermal tolerance under controlled heat stress to strengthen the selection intensity. Furthermore, the phenotypic and the genotypic response to extreme high temperature were investigated based on the comparison of the F-1 progeny of the selected and natural populations in growth, physiology, mortality rate post heat stress, genetic structure, and gene expression. The parameter of growth showed no significant change between the selected and natural populations for the 6-month-old oysters. The selected population exhibited a higher survival rate after exposure to heat stress in the laboratory, which is in line with result of the filed experience that summer mortality of selected population was significantly lower than that of natural population. Further, the respiration rate of the selected population increased at 38 degrees C, while it increased at 35 degrees C in the natural population. Simultaneously, metabolism-related enzymes (PK, SOD) showed higher activity levels in the selected population. Furthermore, phylogenetic analysis, population structure, and principal component analysis (PCA) suggested that the selected and natural populations exhibited genetic divergence, with eight genes (IF4A2, IF6, EIF3A, MANBA, DDX43, RECS, CAT2, ... Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Frontiers in Marine Science 7 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pacific oyster artificial selection thermotolerance genetic structure physiological traits Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences SUMMER MORTALITY CLIMATE-CHANGE OXYGEN-CONSUMPTION LOCAL ADAPTATION STRESS LIMITATION RESISTANCE SELECTION INCREASE THUNBERG |
spellingShingle |
Pacific oyster artificial selection thermotolerance genetic structure physiological traits Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences SUMMER MORTALITY CLIMATE-CHANGE OXYGEN-CONSUMPTION LOCAL ADAPTATION STRESS LIMITATION RESISTANCE SELECTION INCREASE THUNBERG Ding, Fangfang Li, Ao Cong, Rihao Wang, Xinxing Wang, Wei Que, Huayong Zhang, Guofan Li, Li The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas |
topic_facet |
Pacific oyster artificial selection thermotolerance genetic structure physiological traits Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences SUMMER MORTALITY CLIMATE-CHANGE OXYGEN-CONSUMPTION LOCAL ADAPTATION STRESS LIMITATION RESISTANCE SELECTION INCREASE THUNBERG |
description |
Investigating responses of organisms to stressful or new environments with selection pressure is one of the crucial problems in evolutionary biology, and it is of importance to understand the phenotypic and molecular mechanism underlying thermal tolerance under the context of the climate change. The Pacific oyster, Crassostrea gigas, inhabiting the environment with high variation in temperature, is a worldwide aquaculture species. However, summer mortality relevant to the high temperature is one of the problems challenging the oyster industry. An artificial selective breeding program was initiated to select for the thermal tolerance of oysters in an attempt to increase the summer survival rates since 2017 in our study. Parents of thermotolerance oyster selection is are based on acute thermal tolerance under controlled heat stress to strengthen the selection intensity. Furthermore, the phenotypic and the genotypic response to extreme high temperature were investigated based on the comparison of the F-1 progeny of the selected and natural populations in growth, physiology, mortality rate post heat stress, genetic structure, and gene expression. The parameter of growth showed no significant change between the selected and natural populations for the 6-month-old oysters. The selected population exhibited a higher survival rate after exposure to heat stress in the laboratory, which is in line with result of the filed experience that summer mortality of selected population was significantly lower than that of natural population. Further, the respiration rate of the selected population increased at 38 degrees C, while it increased at 35 degrees C in the natural population. Simultaneously, metabolism-related enzymes (PK, SOD) showed higher activity levels in the selected population. Furthermore, phylogenetic analysis, population structure, and principal component analysis (PCA) suggested that the selected and natural populations exhibited genetic divergence, with eight genes (IF4A2, IF6, EIF3A, MANBA, DDX43, RECS, CAT2, ... |
format |
Report |
author |
Ding, Fangfang Li, Ao Cong, Rihao Wang, Xinxing Wang, Wei Que, Huayong Zhang, Guofan Li, Li |
author_facet |
Ding, Fangfang Li, Ao Cong, Rihao Wang, Xinxing Wang, Wei Que, Huayong Zhang, Guofan Li, Li |
author_sort |
Ding, Fangfang |
title |
The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas |
title_short |
The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas |
title_full |
The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas |
title_fullStr |
The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas |
title_sort |
phenotypic and the genetic response to the extreme high temperature provides new insight into thermal tolerance for the pacific oyster crassostrea gigas |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/167947 https://doi.org/10.3389/fmars.2020.00399 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/167947 doi:10.3389/fmars.2020.00399 |
op_doi |
https://doi.org/10.3389/fmars.2020.00399 |
container_title |
Frontiers in Marine Science |
container_volume |
7 |
_version_ |
1766394291168477184 |