Using Microsatellites to Assess Genetic Variation in a Selective Breeding Program of Chinese Bay Scallop (Argopecten irradians irradians)

• Main Authors: Zhang, Shoudu, Li, Li, Zhang, Guofan, Zhang, GF
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2013
• Subjects: Argopecten Irradians Irradians; Selective Breeding Program; Microsatellites; Genetic Variation; Fisheries; Science & Technology; Life Sciences & Biomedicine; OYSTER CRASSOSTREA-GIGAS; EFFECTIVE POPULATION-SIZE; LAMARCK 1819; PACIFIC OYSTER; 2 STOCKS; REALIZED HERITABILITY; TOTAL WEIGHT; SHELL WIDTH; GROWTH-RATE; MARKERS; Lamarck; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: http://ir.qdio.ac.cn/handle/337002/16730

This study aimed to improve our understanding of the genetics of the Chinese bay scallop (Argopecten irradians irradians), one of the most important maricultured shellfish in China. Ten polymorphic microsatellite loci were examined to assess the allelic diversity, heterozygosity, and genetic variation between two domesticated populations selected for fast growth in breeding programs, and their base population. Forty-one alleles were found throughout the loci and the mean number of alleles per locus ranged 3.30-3.50. The average heterozygosity ranged 0.38-0.45, whereas the polymorphic information content ranged 0.1504-0.7518. Genetic differences between the three populations were detected based on the number of alleles per locus, effective number of alleles, Shannon index, inbreeding coefficient (Fis), p values, genetic distance, and pairwise Fst values. There was no significant loss of genetic variability in the breeding program but changes in gene frequencies were detectable over the populations, implying that the loci were affected by the pressures of selective culture. This study aimed to improve our understanding of the genetics of the Chinese bay scallop (Argopecten irradians irradians), one of the most important maricultured shellfish in China. Ten polymorphic microsatellite loci were examined to assess the allelic diversity, heterozygosity, and genetic variation between two domesticated populations selected for fast growth in breeding programs, and their base population. Forty-one alleles were found throughout the loci and the mean number of alleles per locus ranged 3.30-3.50. The average heterozygosity ranged 0.38-0.45, whereas the polymorphic information content ranged 0.1504-0.7518. Genetic differences between the three populations were detected based on the number of alleles per locus, effective number of alleles, Shannon index, inbreeding coefficient (Fis), p values, genetic distance, and pairwise Fst values. There was no significant loss of genetic variability in the breeding program but changes ...