Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas
AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated musc...
Published in: | Journal of Comparative Physiology B |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2013
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/16717 https://doi.org/10.1007/s00360-013-0743-6 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16717 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16717 2023-05-15T15:57:49+02:00 Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-Yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte Corporeau, C 2013-07-01 http://ir.qdio.ac.cn/handle/337002/16717 https://doi.org/10.1007/s00360-013-0743-6 英语 eng JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Ximing; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-Yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas,JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,2013,183(5):597-611 http://ir.qdio.ac.cn/handle/337002/16717 doi:10.1007/s00360-013-0743-6 6 Marine Bivalve Crassostrea Gigas Hypoxia Amp-activated Protein Kinase Alternative Splicing Adductor Muscle Physiology Zoology Science & Technology Life Sciences & Biomedicine METABOLIC-RATE DEPRESSION RAT SKELETAL-MUSCLE GLUCOSE-TRANSPORT REPRODUCTIVE-CYCLE MOLECULAR-CLONING OOCYTE MATURATION CELLULAR-ENERGY GENE-EXPRESSION SUBUNIT GENE AKT ACTIVITY Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1007/s00360-013-0743-6 2022-06-27T05:35:44Z AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPK alpha nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPK alpha and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia. AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Journal of Comparative Physiology B 183 5 597 611 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Marine Bivalve Crassostrea Gigas Hypoxia Amp-activated Protein Kinase Alternative Splicing Adductor Muscle Physiology Zoology Science & Technology Life Sciences & Biomedicine METABOLIC-RATE DEPRESSION RAT SKELETAL-MUSCLE GLUCOSE-TRANSPORT REPRODUCTIVE-CYCLE MOLECULAR-CLONING OOCYTE MATURATION CELLULAR-ENERGY GENE-EXPRESSION SUBUNIT GENE AKT ACTIVITY |
spellingShingle |
Marine Bivalve Crassostrea Gigas Hypoxia Amp-activated Protein Kinase Alternative Splicing Adductor Muscle Physiology Zoology Science & Technology Life Sciences & Biomedicine METABOLIC-RATE DEPRESSION RAT SKELETAL-MUSCLE GLUCOSE-TRANSPORT REPRODUCTIVE-CYCLE MOLECULAR-CLONING OOCYTE MATURATION CELLULAR-ENERGY GENE-EXPRESSION SUBUNIT GENE AKT ACTIVITY Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-Yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte Corporeau, C Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
topic_facet |
Marine Bivalve Crassostrea Gigas Hypoxia Amp-activated Protein Kinase Alternative Splicing Adductor Muscle Physiology Zoology Science & Technology Life Sciences & Biomedicine METABOLIC-RATE DEPRESSION RAT SKELETAL-MUSCLE GLUCOSE-TRANSPORT REPRODUCTIVE-CYCLE MOLECULAR-CLONING OOCYTE MATURATION CELLULAR-ENERGY GENE-EXPRESSION SUBUNIT GENE AKT ACTIVITY |
description |
AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPK alpha nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPK alpha and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia. AMP-activated protein kinase alpha (AMPK alpha) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase alpha (AMPK alpha) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPK alpha. In the smooth muscle, a truncated isoform of AMPK alpha was increased from 1 to 6 h of hypoxia, and was linked ... |
format |
Article in Journal/Newspaper |
author |
Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-Yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte Corporeau, C |
author_facet |
Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-Yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte Corporeau, C |
author_sort |
Guevelou, Eric |
title |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_short |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_full |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_fullStr |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_sort |
regulation of a truncated isoform of amp-activated protein kinase alpha (ampk alpha) in response to hypoxia in the muscle of pacific oyster crassostrea gigas |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16717 https://doi.org/10.1007/s00360-013-0743-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Ximing; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-Yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte.Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPK alpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas,JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,2013,183(5):597-611 http://ir.qdio.ac.cn/handle/337002/16717 doi:10.1007/s00360-013-0743-6 |
op_rights |
6 |
op_doi |
https://doi.org/10.1007/s00360-013-0743-6 |
container_title |
Journal of Comparative Physiology B |
container_volume |
183 |
container_issue |
5 |
container_start_page |
597 |
op_container_end_page |
611 |
_version_ |
1766393511243939840 |