Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development
We characterized the expression patterns of pepsinogen A (tPGA) and pepsinogen C (tPGC) in turbot (Scophthalmus maximus). Quantitative expression analysis showed that tPGC was preferentially expressed in early developmental stages, and that the tPGA mRNA expression level was higher in adult fish. Fu...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16712 2023-05-15T13:40:37+02:00 Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development Chi, Liang Xu, Shihong Xiao, Zhizhong Lin, Fan Ma, Daoyuan Zhao, Chunyan Xiao, Yongshuang Liu, Qinghua Li, Jun Liu, QH 2013-05-01 http://ir.qdio.ac.cn/handle/337002/16712 https://doi.org/10.1016/j.cbpb.2013.03.002 英语 eng COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY Chi, Liang; Xu, Shihong; Xiao, Zhizhong; Lin, Fan; Ma, Daoyuan; Zhao, Chunyan; Xiao, Yongshuang; Liu, Qinghua; Li, Jun.Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2013,165(1):58-65 http://ir.qdio.ac.cn/handle/337002/16712 doi:10.1016/j.cbpb.2013.03.002 6 Turbot (Scophthalmus Maximus) Pepsinogen a Pepsinogen c Functional Differences Development Biochemistry & Molecular Biology Zoology Science & Technology Life Sciences & Biomedicine ORANGE-SPOTTED GROUPER PORGY PAGRUS-PAGRUS DIGESTIVE ENZYMES EPINEPHELUS-COIOIDES GASTRIC-MUCOSA ANTARCTIC FISH EVOLUTION ONTOGENY IDENTIFICATION FUNCTIONALITY Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1016/j.cbpb.2013.03.002 2022-06-27T05:35:44Z We characterized the expression patterns of pepsinogen A (tPGA) and pepsinogen C (tPGC) in turbot (Scophthalmus maximus). Quantitative expression analysis showed that tPGC was preferentially expressed in early developmental stages, and that the tPGA mRNA expression level was higher in adult fish. Full-length cDNA constructs of tPGA and tPGC were 1307 bp (from which 377 amino acids were deduced); and 1430 bp (from which 385 amino acids were deduced), respectively. The deduced proteins of tPGA and tPGC possessed signal peptides of 17 amino acids and 20 amino acids respectively. The initial transcripts of tPGA and tPGC were detected at 22 days post hatching (dph), well after the formation of gastric glands (16 dph). This suggested that the morphologic development of gastric glands was not synchronous with their functional development. In addition, tPGA and tPGC mRNAs were also expressed in muscle and ovary at much lower levels than in stomach and esophagus. The distribution of tPGA and tPGC in the turbot was investigated using in-situ hybridization, and tPGA and tPGC were first detected in the esophagus and cardiac region of the stomach, and then throughout the stomach. (C) 2013 Elsevier Inc. All rights reserved. We characterized the expression patterns of pepsinogen A (tPGA) and pepsinogen C (tPGC) in turbot (Scophthalmus maximus). Quantitative expression analysis showed that tPGC was preferentially expressed in early developmental stages, and that the tPGA mRNA expression level was higher in adult fish. Full-length cDNA constructs of tPGA and tPGC were 1307 bp (from which 377 amino acids were deduced); and 1430 bp (from which 385 amino acids were deduced), respectively. The deduced proteins of tPGA and tPGC possessed signal peptides of 17 amino acids and 20 amino acids respectively. The initial transcripts of tPGA and tPGC were detected at 22 days post hatching (dph), well after the formation of gastric glands (16 dph). This suggested that the morphologic development of gastric glands was not synchronous with ... Article in Journal/Newspaper Antarc* Antarctic Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Antarctic Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 165 1 58 65 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Turbot (Scophthalmus Maximus) Pepsinogen a Pepsinogen c Functional Differences Development Biochemistry & Molecular Biology Zoology Science & Technology Life Sciences & Biomedicine ORANGE-SPOTTED GROUPER PORGY PAGRUS-PAGRUS DIGESTIVE ENZYMES EPINEPHELUS-COIOIDES GASTRIC-MUCOSA ANTARCTIC FISH EVOLUTION ONTOGENY IDENTIFICATION FUNCTIONALITY |
spellingShingle |
Turbot (Scophthalmus Maximus) Pepsinogen a Pepsinogen c Functional Differences Development Biochemistry & Molecular Biology Zoology Science & Technology Life Sciences & Biomedicine ORANGE-SPOTTED GROUPER PORGY PAGRUS-PAGRUS DIGESTIVE ENZYMES EPINEPHELUS-COIOIDES GASTRIC-MUCOSA ANTARCTIC FISH EVOLUTION ONTOGENY IDENTIFICATION FUNCTIONALITY Chi, Liang Xu, Shihong Xiao, Zhizhong Lin, Fan Ma, Daoyuan Zhao, Chunyan Xiao, Yongshuang Liu, Qinghua Li, Jun Liu, QH Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development |
topic_facet |
Turbot (Scophthalmus Maximus) Pepsinogen a Pepsinogen c Functional Differences Development Biochemistry & Molecular Biology Zoology Science & Technology Life Sciences & Biomedicine ORANGE-SPOTTED GROUPER PORGY PAGRUS-PAGRUS DIGESTIVE ENZYMES EPINEPHELUS-COIOIDES GASTRIC-MUCOSA ANTARCTIC FISH EVOLUTION ONTOGENY IDENTIFICATION FUNCTIONALITY |
description |
We characterized the expression patterns of pepsinogen A (tPGA) and pepsinogen C (tPGC) in turbot (Scophthalmus maximus). Quantitative expression analysis showed that tPGC was preferentially expressed in early developmental stages, and that the tPGA mRNA expression level was higher in adult fish. Full-length cDNA constructs of tPGA and tPGC were 1307 bp (from which 377 amino acids were deduced); and 1430 bp (from which 385 amino acids were deduced), respectively. The deduced proteins of tPGA and tPGC possessed signal peptides of 17 amino acids and 20 amino acids respectively. The initial transcripts of tPGA and tPGC were detected at 22 days post hatching (dph), well after the formation of gastric glands (16 dph). This suggested that the morphologic development of gastric glands was not synchronous with their functional development. In addition, tPGA and tPGC mRNAs were also expressed in muscle and ovary at much lower levels than in stomach and esophagus. The distribution of tPGA and tPGC in the turbot was investigated using in-situ hybridization, and tPGA and tPGC were first detected in the esophagus and cardiac region of the stomach, and then throughout the stomach. (C) 2013 Elsevier Inc. All rights reserved. We characterized the expression patterns of pepsinogen A (tPGA) and pepsinogen C (tPGC) in turbot (Scophthalmus maximus). Quantitative expression analysis showed that tPGC was preferentially expressed in early developmental stages, and that the tPGA mRNA expression level was higher in adult fish. Full-length cDNA constructs of tPGA and tPGC were 1307 bp (from which 377 amino acids were deduced); and 1430 bp (from which 385 amino acids were deduced), respectively. The deduced proteins of tPGA and tPGC possessed signal peptides of 17 amino acids and 20 amino acids respectively. The initial transcripts of tPGA and tPGC were detected at 22 days post hatching (dph), well after the formation of gastric glands (16 dph). This suggested that the morphologic development of gastric glands was not synchronous with ... |
format |
Article in Journal/Newspaper |
author |
Chi, Liang Xu, Shihong Xiao, Zhizhong Lin, Fan Ma, Daoyuan Zhao, Chunyan Xiao, Yongshuang Liu, Qinghua Li, Jun Liu, QH |
author_facet |
Chi, Liang Xu, Shihong Xiao, Zhizhong Lin, Fan Ma, Daoyuan Zhao, Chunyan Xiao, Yongshuang Liu, Qinghua Li, Jun Liu, QH |
author_sort |
Chi, Liang |
title |
Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development |
title_short |
Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development |
title_full |
Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development |
title_fullStr |
Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development |
title_full_unstemmed |
Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development |
title_sort |
pepsinogen a and c genes in turbot (scophthalmus maximus): characterization and expression in early development |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16712 https://doi.org/10.1016/j.cbpb.2013.03.002 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Scophthalmus maximus Turbot |
genre_facet |
Antarc* Antarctic Scophthalmus maximus Turbot |
op_relation |
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY Chi, Liang; Xu, Shihong; Xiao, Zhizhong; Lin, Fan; Ma, Daoyuan; Zhao, Chunyan; Xiao, Yongshuang; Liu, Qinghua; Li, Jun.Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2013,165(1):58-65 http://ir.qdio.ac.cn/handle/337002/16712 doi:10.1016/j.cbpb.2013.03.002 |
op_rights |
6 |
op_doi |
https://doi.org/10.1016/j.cbpb.2013.03.002 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
165 |
container_issue |
1 |
container_start_page |
58 |
op_container_end_page |
65 |
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1766137520745086976 |