Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular bree...
Published in: | Marine Biotechnology |
---|---|
Main Authors: | , , , , |
Format: | Report |
Language: | English |
Published: |
SPRINGER
2020
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/167062 http://ir.qdio.ac.cn/handle/337002/167063 https://doi.org/10.1007/s10126-019-09942-6 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/167063 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/167063 2023-05-15T15:57:44+02:00 Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq Liu, Youli Zhu, Qihui Li, Li Wang, Wei Zhang, Guofan 2020-04-01 http://ir.qdio.ac.cn/handle/337002/167062 http://ir.qdio.ac.cn/handle/337002/167063 https://doi.org/10.1007/s10126-019-09942-6 英语 eng SPRINGER MARINE BIOTECHNOLOGY http://ir.qdio.ac.cn/handle/337002/167062 http://ir.qdio.ac.cn/handle/337002/167063 doi:10.1007/s10126-019-09942-6 HSF1 binding site Thermal stress HSP Regulatory relationship Crassostrea gigas ChIP-seq Biotechnology & Applied Microbiology Marine & Freshwater Biology 期刊论文 2020 ftchinacasciocas https://doi.org/10.1007/s10126-019-09942-6 2022-06-27T05:42:35Z The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance. However, the regulatory relationship between the expansion of heat shock protein (HSP) HSP 70 and HSF1 is not yet clear in C. gigas. In this study, we analyzed genes regulated by HSF1 in response to heat shock by chromatin immunoprecipitation followed by sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to HSF1 binding sites, and these peaks were annotated to the nearest genes. In Gene Ontology analysis, HSF1 target genes were related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Marine Biotechnology 22 2 167 179 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
HSF1 binding site Thermal stress HSP Regulatory relationship Crassostrea gigas ChIP-seq Biotechnology & Applied Microbiology Marine & Freshwater Biology |
spellingShingle |
HSF1 binding site Thermal stress HSP Regulatory relationship Crassostrea gigas ChIP-seq Biotechnology & Applied Microbiology Marine & Freshwater Biology Liu, Youli Zhu, Qihui Li, Li Wang, Wei Zhang, Guofan Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq |
topic_facet |
HSF1 binding site Thermal stress HSP Regulatory relationship Crassostrea gigas ChIP-seq Biotechnology & Applied Microbiology Marine & Freshwater Biology |
description |
The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance. However, the regulatory relationship between the expansion of heat shock protein (HSP) HSP 70 and HSF1 is not yet clear in C. gigas. In this study, we analyzed genes regulated by HSF1 in response to heat shock by chromatin immunoprecipitation followed by sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to HSF1 binding sites, and these peaks were annotated to the nearest genes. In Gene Ontology analysis, HSF1 target genes were related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster. |
format |
Report |
author |
Liu, Youli Zhu, Qihui Li, Li Wang, Wei Zhang, Guofan |
author_facet |
Liu, Youli Zhu, Qihui Li, Li Wang, Wei Zhang, Guofan |
author_sort |
Liu, Youli |
title |
Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq |
title_short |
Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq |
title_full |
Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq |
title_fullStr |
Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq |
title_full_unstemmed |
Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq |
title_sort |
identification of hsf1 target genes involved in thermal stress in the pacific oyster crassostrea gigas by chip-seq |
publisher |
SPRINGER |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/167062 http://ir.qdio.ac.cn/handle/337002/167063 https://doi.org/10.1007/s10126-019-09942-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
MARINE BIOTECHNOLOGY http://ir.qdio.ac.cn/handle/337002/167062 http://ir.qdio.ac.cn/handle/337002/167063 doi:10.1007/s10126-019-09942-6 |
op_doi |
https://doi.org/10.1007/s10126-019-09942-6 |
container_title |
Marine Biotechnology |
container_volume |
22 |
container_issue |
2 |
container_start_page |
167 |
op_container_end_page |
179 |
_version_ |
1766393433813942272 |