Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq

The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular bree...

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Published in:Marine Biotechnology
Main Authors: Liu, Youli, Zhu, Qihui, Li, Li, Wang, Wei, Zhang, Guofan
Format: Report
Language:English
Published: SPRINGER 2020
Subjects:
HSF1 binding site
Thermal stress
HSP
Regulatory relationship
Crassostrea gigas
ChIP-seq
Biotechnology & Applied Microbiology
Marine & Freshwater Biology
HEAT-SHOCK FACTOR
GENETICALLY-BASED RESISTANCE
DNA-BINDING
MOLECULAR CHAPERONES
IN-VIVO
TRANSCRIPTION
PROTEIN
EXPRESSION
ACTIVATION
RESPONSES
Pacific
Pacific oyster
Online Access:http://ir.qdio.ac.cn/handle/337002/167061
https://doi.org/10.1007/s10126-019-09942-6
id ftchinacasciocas:oai:ir.qdio.ac.cn:337002/167061
record_format openpolar
spelling ftchinacasciocas:oai:ir.qdio.ac.cn:337002/167061 2023-05-15T15:57:47+02:00 Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq Liu, Youli Zhu, Qihui Li, Li Wang, Wei Zhang, Guofan 2020-04-01 http://ir.qdio.ac.cn/handle/337002/167061 https://doi.org/10.1007/s10126-019-09942-6 英语 eng SPRINGER MARINE BIOTECHNOLOGY http://ir.qdio.ac.cn/handle/337002/167061 doi:10.1007/s10126-019-09942-6 HSF1 binding site Thermal stress HSP Regulatory relationship Crassostrea gigas ChIP-seq Biotechnology & Applied Microbiology Marine & Freshwater Biology HEAT-SHOCK FACTOR GENETICALLY-BASED RESISTANCE DNA-BINDING MOLECULAR CHAPERONES IN-VIVO TRANSCRIPTION PROTEIN EXPRESSION ACTIVATION RESPONSES 期刊论文 2020 ftchinacasciocas https://doi.org/10.1007/s10126-019-09942-6 2022-06-27T05:42:35Z The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance. However, the regulatory relationship between the expansion of heat shock protein (HSP) HSP 70 and HSF1 is not yet clear in C. gigas. In this study, we analyzed genes regulated by HSF1 in response to heat shock by chromatin immunoprecipitation followed by sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to HSF1 binding sites, and these peaks were annotated to the nearest genes. In Gene Ontology analysis, HSF1 target genes were related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Marine Biotechnology 22 2 167 179
institution Open Polar
collection Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR
op_collection_id ftchinacasciocas
language English
topic HSF1 binding site
Thermal stress
HSP
Regulatory relationship
Crassostrea gigas
ChIP-seq
Biotechnology & Applied Microbiology
Marine & Freshwater Biology
HEAT-SHOCK FACTOR
GENETICALLY-BASED RESISTANCE
DNA-BINDING
MOLECULAR CHAPERONES
IN-VIVO
TRANSCRIPTION
PROTEIN
EXPRESSION
ACTIVATION
RESPONSES
spellingShingle HSF1 binding site
Thermal stress
HSP
Regulatory relationship
Crassostrea gigas
ChIP-seq
Biotechnology & Applied Microbiology
Marine & Freshwater Biology
HEAT-SHOCK FACTOR
GENETICALLY-BASED RESISTANCE
DNA-BINDING
MOLECULAR CHAPERONES
IN-VIVO
TRANSCRIPTION
PROTEIN
EXPRESSION
ACTIVATION
RESPONSES
Liu, Youli
Zhu, Qihui
Li, Li
Wang, Wei
Zhang, Guofan
Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
topic_facet HSF1 binding site
Thermal stress
HSP
Regulatory relationship
Crassostrea gigas
ChIP-seq
Biotechnology & Applied Microbiology
Marine & Freshwater Biology
HEAT-SHOCK FACTOR
GENETICALLY-BASED RESISTANCE
DNA-BINDING
MOLECULAR CHAPERONES
IN-VIVO
TRANSCRIPTION
PROTEIN
EXPRESSION
ACTIVATION
RESPONSES
description The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance. However, the regulatory relationship between the expansion of heat shock protein (HSP) HSP 70 and HSF1 is not yet clear in C. gigas. In this study, we analyzed genes regulated by HSF1 in response to heat shock by chromatin immunoprecipitation followed by sequencing (ChIP-seq), determined the expression patterns of target genes by qRT-PCR, and validated the regulatory relationship between one HSP70 and HSF1. We found 916 peaks corresponding to HSF1 binding sites, and these peaks were annotated to the nearest genes. In Gene Ontology analysis, HSF1 target genes were related to signal transduction, energy production, and response to biotic stimulus. Four HSP70 genes, two HSP40 genes, and one small HSP gene exhibited binding to HSF1. One HSP70 with a binding site in the promoter region was validated to be regulated by HSF1 under heat shock. These results provide a basis for future studies aimed at determining the mechanisms underlying thermal tolerance and provide insights into gene regulation in the Pacific oyster.
format Report
author Liu, Youli
Zhu, Qihui
Li, Li
Wang, Wei
Zhang, Guofan
author_facet Liu, Youli
Zhu, Qihui
Li, Li
Wang, Wei
Zhang, Guofan
author_sort Liu, Youli
title Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
title_short Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
title_full Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
title_fullStr Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
title_full_unstemmed Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq
title_sort identification of hsf1 target genes involved in thermal stress in the pacific oyster crassostrea gigas by chip-seq
publisher SPRINGER
publishDate 2020
url http://ir.qdio.ac.cn/handle/337002/167061
https://doi.org/10.1007/s10126-019-09942-6
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_relation MARINE BIOTECHNOLOGY
http://ir.qdio.ac.cn/handle/337002/167061
doi:10.1007/s10126-019-09942-6
op_doi https://doi.org/10.1007/s10126-019-09942-6
container_title Marine Biotechnology
container_volume 22
container_issue 2
container_start_page 167
op_container_end_page 179
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