Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation
Oyster Crassostrea gigas, is considered as a useful environmental indicator since it is widely distributed along the intertidal zone whereby it tends to accumulate cadmium and is always exposed to various pathogen agents. However, its molecular responses to both cadmium and pathogen stimulation rema...
Published in: | Fish & Shellfish Immunology |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2020
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Online Access: | http://ir.qdio.ac.cn/handle/337002/167050 http://ir.qdio.ac.cn/handle/337002/167051 https://doi.org/10.1016/j.fsi.2019.12.083 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/167051 2023-05-15T15:57:57+02:00 Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation Qiu, Limei Chen, Hao Zhou, Zhi Zhang, Huan Liu, Rui Yi, Qilin Yang, Chuanyan Gao, Lei Wang, Lingling 2020-03-01 http://ir.qdio.ac.cn/handle/337002/167050 http://ir.qdio.ac.cn/handle/337002/167051 https://doi.org/10.1016/j.fsi.2019.12.083 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/167050 http://ir.qdio.ac.cn/handle/337002/167051 doi:10.1016/j.fsi.2019.12.083 Crassostrea gigas Cadmium exposure Bacteria challenge Immune response Neuro-endocrine-immune regulatory network Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences 期刊论文 2020 ftchinacasciocas https://doi.org/10.1016/j.fsi.2019.12.083 2022-06-27T05:42:35Z Oyster Crassostrea gigas, is considered as a useful environmental indicator since it is widely distributed along the intertidal zone whereby it tends to accumulate cadmium and is always exposed to various pathogen agents. However, its molecular responses to both cadmium and pathogen stimulation remain unclear. In the present study, transcriptome data of hemocytes from oysters were analyzed to reveal specific molecular responses of oyster to cadmium or cadmium/bacteria stimulation. A total of 21591, 22872 and 20107 genes were detected in the BLANK, Cd24h and Cd/Bac24h group, respectively. Among them, there were 685 differentially expressed genes collected in the comparison of Cd24h versus BLANK. GO analysis of these genes found that sixteen terms into the Molecular Function category displayed transporter activities, and were all over-enrichment by cadmium exposure, whereas twelve terms into Biological Process category involved mainly in metabolic process of the various cellular components and two terms into Cellular Component category were all under-enrichment. The 330 immune responsive genes were shared by two gene lists of CdBac24h versus BLANK and CdBac24h versus Cd24h, and seven out of thirty terms in GO analysis were related to the immune process. Further annotation of these genes from the KEGG database revealed fourteen pathways, including two nervous system related pathways, arachidonic acid pathway, four immune pathways, MAPK cascade and other four cell signaling pathways, and two energy related pathways. Twenty-two differentially expressed genes were identified to responsive to both cadmium exposure and bacteria stimulation, but in different expression patterns, suggesting that bilateral responsive genes, such as alkaline phosphatase and sodium and chloride-dependent glycine transporter gene, could be candidate biomarkers for early warning of cadmium pollution. The present results collectively indicated that a profound neuro-endocrine-immune regulatory network was activated in response to cadmium and ... Report Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 98 138 146 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea gigas Cadmium exposure Bacteria challenge Immune response Neuro-endocrine-immune regulatory network Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Crassostrea gigas Cadmium exposure Bacteria challenge Immune response Neuro-endocrine-immune regulatory network Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Qiu, Limei Chen, Hao Zhou, Zhi Zhang, Huan Liu, Rui Yi, Qilin Yang, Chuanyan Gao, Lei Wang, Lingling Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation |
topic_facet |
Crassostrea gigas Cadmium exposure Bacteria challenge Immune response Neuro-endocrine-immune regulatory network Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Oyster Crassostrea gigas, is considered as a useful environmental indicator since it is widely distributed along the intertidal zone whereby it tends to accumulate cadmium and is always exposed to various pathogen agents. However, its molecular responses to both cadmium and pathogen stimulation remain unclear. In the present study, transcriptome data of hemocytes from oysters were analyzed to reveal specific molecular responses of oyster to cadmium or cadmium/bacteria stimulation. A total of 21591, 22872 and 20107 genes were detected in the BLANK, Cd24h and Cd/Bac24h group, respectively. Among them, there were 685 differentially expressed genes collected in the comparison of Cd24h versus BLANK. GO analysis of these genes found that sixteen terms into the Molecular Function category displayed transporter activities, and were all over-enrichment by cadmium exposure, whereas twelve terms into Biological Process category involved mainly in metabolic process of the various cellular components and two terms into Cellular Component category were all under-enrichment. The 330 immune responsive genes were shared by two gene lists of CdBac24h versus BLANK and CdBac24h versus Cd24h, and seven out of thirty terms in GO analysis were related to the immune process. Further annotation of these genes from the KEGG database revealed fourteen pathways, including two nervous system related pathways, arachidonic acid pathway, four immune pathways, MAPK cascade and other four cell signaling pathways, and two energy related pathways. Twenty-two differentially expressed genes were identified to responsive to both cadmium exposure and bacteria stimulation, but in different expression patterns, suggesting that bilateral responsive genes, such as alkaline phosphatase and sodium and chloride-dependent glycine transporter gene, could be candidate biomarkers for early warning of cadmium pollution. The present results collectively indicated that a profound neuro-endocrine-immune regulatory network was activated in response to cadmium and ... |
format |
Report |
author |
Qiu, Limei Chen, Hao Zhou, Zhi Zhang, Huan Liu, Rui Yi, Qilin Yang, Chuanyan Gao, Lei Wang, Lingling |
author_facet |
Qiu, Limei Chen, Hao Zhou, Zhi Zhang, Huan Liu, Rui Yi, Qilin Yang, Chuanyan Gao, Lei Wang, Lingling |
author_sort |
Qiu, Limei |
title |
Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation |
title_short |
Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation |
title_full |
Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation |
title_fullStr |
Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation |
title_full_unstemmed |
Transcriptomic profile of oyster Crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation |
title_sort |
transcriptomic profile of oyster crassostrea gigas hemocyte after short-term cadmium exposure and bacteria stimulation |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/167050 http://ir.qdio.ac.cn/handle/337002/167051 https://doi.org/10.1016/j.fsi.2019.12.083 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/167050 http://ir.qdio.ac.cn/handle/337002/167051 doi:10.1016/j.fsi.2019.12.083 |
op_doi |
https://doi.org/10.1016/j.fsi.2019.12.083 |
container_title |
Fish & Shellfish Immunology |
container_volume |
98 |
container_start_page |
138 |
op_container_end_page |
146 |
_version_ |
1766393655953719296 |