Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas
Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 prote...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16697 2023-05-15T15:57:45+02:00 Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan Li, L 2013 http://ir.qdio.ac.cn/handle/337002/16697 英语 eng CURRENT ZOOLOGY Wang, Xiaotong; Song, Xiaorui; Wang, Tong; Zhu, Qihui; Miao, Guoying; Chen, Yuanxin; Fang, Xiaodong; Que, Huayong; Li, Li; Zhang, Guofan.Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas,CURRENT ZOOLOGY,2013,59(1):109-115 http://ir.qdio.ac.cn/handle/337002/16697 6 Mollusca Crassostrea Gigas Pif97 Gene Biomineralization Shell Formation Calcite Zoology Science & Technology Life Sciences & Biomedicine MATRIX PROTEIN SHELL NACRE MOLLUSKS CRYSTALS Article 期刊论文 2013 ftchinacasciocas 2022-06-27T05:35:44Z Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue (cgPif97) shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages indicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was affected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose increased, suggesting that the cgPif97 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 (1): 109-115, 2013]. Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Mollusca Crassostrea Gigas Pif97 Gene Biomineralization Shell Formation Calcite Zoology Science & Technology Life Sciences & Biomedicine MATRIX PROTEIN SHELL NACRE MOLLUSKS CRYSTALS |
spellingShingle |
Mollusca Crassostrea Gigas Pif97 Gene Biomineralization Shell Formation Calcite Zoology Science & Technology Life Sciences & Biomedicine MATRIX PROTEIN SHELL NACRE MOLLUSKS CRYSTALS Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan Li, L Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
topic_facet |
Mollusca Crassostrea Gigas Pif97 Gene Biomineralization Shell Formation Calcite Zoology Science & Technology Life Sciences & Biomedicine MATRIX PROTEIN SHELL NACRE MOLLUSKS CRYSTALS |
description |
Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure between the two species. This homologue (cgPif97) shares characteristics with pfPif97, and suggests that the biological functions of these two proteins may be similar. The expression pattern of cgPif97 in different tissues and development stages indicates that it may play an important role in shell formation of the adult oyster. The morphology of the inner shell surface was affected by injected siRNA of cgPif97 and the calcite laths of the shell became thinner and narrower when the siRNA dose increased, suggesting that the cgPif97 gene plays an important role in calcite shell formation in C. gigas. In conclusion, we found evidence that the Pif177 gene evolved very fast but still retains a similar function among species [Current Zoology 59 (1): 109-115, 2013]. Mollusc shell matrix proteins (SMPs) are important functional components embedded in the shell and play a role in shell formation. A SMP (Pif177) was identified previously from the nacreous layer of the Japanese pearl oyster Pinctada fucata, and its cleavage products (named pfPif97 and pfPif80 proteins) were found to bind to the chitin framework and induce aragonite crystal formation and orient the c axis. In this study, a homologue of pfPif177 was cloned from the mantle of the Pacific oyster Crassostrea gigas, containing the homologue of pfPif97 only and not pfPif80. This finding hints at the large divergence in gene structure ... |
format |
Article in Journal/Newspaper |
author |
Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan Li, L |
author_facet |
Wang, Xiaotong Song, Xiaorui Wang, Tong Zhu, Qihui Miao, Guoying Chen, Yuanxin Fang, Xiaodong Que, Huayong Li, Li Zhang, Guofan Li, L |
author_sort |
Wang, Xiaotong |
title |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_short |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_full |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_fullStr |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas |
title_sort |
evolution and functional analysis of the pif97 gene of the pacific oyster crassostrea gigas |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16697 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
CURRENT ZOOLOGY Wang, Xiaotong; Song, Xiaorui; Wang, Tong; Zhu, Qihui; Miao, Guoying; Chen, Yuanxin; Fang, Xiaodong; Que, Huayong; Li, Li; Zhang, Guofan.Evolution and functional analysis of the Pif97 gene of the Pacific oyster Crassostrea gigas,CURRENT ZOOLOGY,2013,59(1):109-115 http://ir.qdio.ac.cn/handle/337002/16697 |
op_rights |
6 |
_version_ |
1766393440930627584 |