The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge
In the present study, the expression of some immune-related genes was examined as indicator to understand the development of immune defense system during the ontogenesis of scallop Chlamys farreri. The mRNA transcripts of pattern-recognition receptors (PRRs) including CfPGRP-S1, CfLGBP, CfLec-1 and...
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Online Access: | http://ir.qdio.ac.cn/handle/337002/16646 https://doi.org/10.1016/j.fsi.2012.12.023 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16646 2023-05-15T15:59:10+02:00 The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge Yue, Feng Shi, Xiaowei Zhou, Zhi Wang, Lingling Wang, Mengqiang Yang, Jialong Qiu, Limei Song, Linsheng Song, LS 2013-03-01 http://ir.qdio.ac.cn/handle/337002/16646 https://doi.org/10.1016/j.fsi.2012.12.023 英语 eng FISH & SHELLFISH IMMUNOLOGY Yue, Feng; Shi, Xiaowei; Zhou, Zhi; Wang, Lingling; Wang, Mengqiang; Yang, Jialong; Qiu, Limei; Song, Linsheng.The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge,FISH & SHELLFISH IMMUNOLOGY,2013,34(3):855-864 http://ir.qdio.ac.cn/handle/337002/16646 doi:10.1016/j.fsi.2012.12.023 6 Chlamys Farreri Larval Development Immune Defense System Bacterial Challenge Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine PEPTIDOGLYCAN RECOGNITION PROTEIN PERMEABILITY-INCREASING PROTEIN MYTILUS-TROSSULUS MOLLUSCA MESSENGER-RNA EXPRESSION OYSTER CRASSOSTREA-GIGAS C-TYPE LECTIN MOLECULAR-CLONING MARINE-INVERTEBRATES RECOMBINANT PROTEIN CDNA CLONING Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1016/j.fsi.2012.12.023 2022-06-27T05:35:44Z In the present study, the expression of some immune-related genes was examined as indicator to understand the development of immune defense system during the ontogenesis of scallop Chlamys farreri. The mRNA transcripts of pattern-recognition receptors (PRRs) including CfPGRP-S1, CfLGBP, CfLec-1 and CfLec-3 were observed at a low level or even undetected at early developmental stages from eggs to blastula, and then began to increase overwhelmingly in trochophore. For the genes of immune effector including CfLYZ, CfLBP/BPI, CfSOD and CfCAT, their mRNA transcripts were higher expressed in embryos, and increased significantly in D-hinged or early veliger larvae. The whole-mount immunofluorescence assay revealed two immunoreactive spots of CfPGRP-S1 were first observed in the mid-ventral region of prototroch in trochophore, and the immunopositive fluorescence of CfLGBP, CfLec-1 and CfLec-3 appeared at the same spots in early D-hinged larvae. Most of the PRRs were located in velum, mouth, esophagus and stomach region in early and mid-veliger larvae, and especially the strong immunopositive fluorescence of CfLec-3 was observed in velum. The immunoreactive areas of CfLYZ, CfLBP/BPI, CfSOD and CfCAT were observed in trochophore and early D-hinged larvae. After D-hinged larvae, they distributed in different tissues from the edge of velum, mouth, esophagus to the region around digestive gland. After bacterial challenge, the mRNA expression of CfLGBP, CfLec-1 and CfLec-3 did not change significantly in trochophore, while a down-regulation of CfPGRP-S1 was observed at 6 h (P < 0.05). The expression of CfPGRP-S1 and CfLGBP decreased or increased inversely in D-hinged and late veliger larvae respectively, whereas CfLec-1 and CfLec-3 increased significantly during 6-24 h after bacterial challenge in the two stages (P < 0.05). In contrast, the expressions of immune effectors in trochophore and late veliger larvae were significant up-regulated at 6 h, 12 h or 24 h after bacterial challenge (P < 0.05). However, in late ... Article in Journal/Newspaper Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 34 3 855 864 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Chlamys Farreri Larval Development Immune Defense System Bacterial Challenge Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine PEPTIDOGLYCAN RECOGNITION PROTEIN PERMEABILITY-INCREASING PROTEIN MYTILUS-TROSSULUS MOLLUSCA MESSENGER-RNA EXPRESSION OYSTER CRASSOSTREA-GIGAS C-TYPE LECTIN MOLECULAR-CLONING MARINE-INVERTEBRATES RECOMBINANT PROTEIN CDNA CLONING |
spellingShingle |
Chlamys Farreri Larval Development Immune Defense System Bacterial Challenge Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine PEPTIDOGLYCAN RECOGNITION PROTEIN PERMEABILITY-INCREASING PROTEIN MYTILUS-TROSSULUS MOLLUSCA MESSENGER-RNA EXPRESSION OYSTER CRASSOSTREA-GIGAS C-TYPE LECTIN MOLECULAR-CLONING MARINE-INVERTEBRATES RECOMBINANT PROTEIN CDNA CLONING Yue, Feng Shi, Xiaowei Zhou, Zhi Wang, Lingling Wang, Mengqiang Yang, Jialong Qiu, Limei Song, Linsheng Song, LS The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge |
topic_facet |
Chlamys Farreri Larval Development Immune Defense System Bacterial Challenge Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine PEPTIDOGLYCAN RECOGNITION PROTEIN PERMEABILITY-INCREASING PROTEIN MYTILUS-TROSSULUS MOLLUSCA MESSENGER-RNA EXPRESSION OYSTER CRASSOSTREA-GIGAS C-TYPE LECTIN MOLECULAR-CLONING MARINE-INVERTEBRATES RECOMBINANT PROTEIN CDNA CLONING |
description |
In the present study, the expression of some immune-related genes was examined as indicator to understand the development of immune defense system during the ontogenesis of scallop Chlamys farreri. The mRNA transcripts of pattern-recognition receptors (PRRs) including CfPGRP-S1, CfLGBP, CfLec-1 and CfLec-3 were observed at a low level or even undetected at early developmental stages from eggs to blastula, and then began to increase overwhelmingly in trochophore. For the genes of immune effector including CfLYZ, CfLBP/BPI, CfSOD and CfCAT, their mRNA transcripts were higher expressed in embryos, and increased significantly in D-hinged or early veliger larvae. The whole-mount immunofluorescence assay revealed two immunoreactive spots of CfPGRP-S1 were first observed in the mid-ventral region of prototroch in trochophore, and the immunopositive fluorescence of CfLGBP, CfLec-1 and CfLec-3 appeared at the same spots in early D-hinged larvae. Most of the PRRs were located in velum, mouth, esophagus and stomach region in early and mid-veliger larvae, and especially the strong immunopositive fluorescence of CfLec-3 was observed in velum. The immunoreactive areas of CfLYZ, CfLBP/BPI, CfSOD and CfCAT were observed in trochophore and early D-hinged larvae. After D-hinged larvae, they distributed in different tissues from the edge of velum, mouth, esophagus to the region around digestive gland. After bacterial challenge, the mRNA expression of CfLGBP, CfLec-1 and CfLec-3 did not change significantly in trochophore, while a down-regulation of CfPGRP-S1 was observed at 6 h (P < 0.05). The expression of CfPGRP-S1 and CfLGBP decreased or increased inversely in D-hinged and late veliger larvae respectively, whereas CfLec-1 and CfLec-3 increased significantly during 6-24 h after bacterial challenge in the two stages (P < 0.05). In contrast, the expressions of immune effectors in trochophore and late veliger larvae were significant up-regulated at 6 h, 12 h or 24 h after bacterial challenge (P < 0.05). However, in late ... |
format |
Article in Journal/Newspaper |
author |
Yue, Feng Shi, Xiaowei Zhou, Zhi Wang, Lingling Wang, Mengqiang Yang, Jialong Qiu, Limei Song, Linsheng Song, LS |
author_facet |
Yue, Feng Shi, Xiaowei Zhou, Zhi Wang, Lingling Wang, Mengqiang Yang, Jialong Qiu, Limei Song, Linsheng Song, LS |
author_sort |
Yue, Feng |
title |
The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge |
title_short |
The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge |
title_full |
The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge |
title_fullStr |
The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge |
title_full_unstemmed |
The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge |
title_sort |
expression of immune-related genes during the ontogenesis of scallop chlamys farreri and their response to bacterial challenge |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16646 https://doi.org/10.1016/j.fsi.2012.12.023 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Yue, Feng; Shi, Xiaowei; Zhou, Zhi; Wang, Lingling; Wang, Mengqiang; Yang, Jialong; Qiu, Limei; Song, Linsheng.The expression of immune-related genes during the ontogenesis of scallop Chlamys farreri and their response to bacterial challenge,FISH & SHELLFISH IMMUNOLOGY,2013,34(3):855-864 http://ir.qdio.ac.cn/handle/337002/16646 doi:10.1016/j.fsi.2012.12.023 |
op_rights |
6 |
op_doi |
https://doi.org/10.1016/j.fsi.2012.12.023 |
container_title |
Fish & Shellfish Immunology |
container_volume |
34 |
container_issue |
3 |
container_start_page |
855 |
op_container_end_page |
864 |
_version_ |
1766394955338612736 |