Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection
Disease outbreaks caused by iridoviruses are known to affect farmed flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). To facilitate quantitative real time RT-PCR (qRT-PCR) analysis of gene expression in flounder and turbot during viral infection, we in this study examined the pote...
Published in: | Veterinary Immunology and Immunopathology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16627 2023-05-15T18:15:41+02:00 Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection Zhang, Jian Hu, Yong-hua Sun, Bo-guang Xiao, Zhi-zhong Sun, Li Sun, L 2013-04-15 http://ir.qdio.ac.cn/handle/337002/16627 https://doi.org/10.1016/j.vetimm.2012.12.018 英语 eng VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY Zhang, Jian; Hu, Yong-hua; Sun, Bo-guang; Xiao, Zhi-zhong; Sun, Li.Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2013,152(41702):303-316 http://ir.qdio.ac.cn/handle/337002/16627 doi:10.1016/j.vetimm.2012.12.018 6 Quantitative Real Time Rt-pcr Reference Gene Housekeeping Gene Paralichthys Olivaceus Scophthalmus Maximus Immunology Veterinary Sciences Science & Technology Life Sciences & Biomedicine POTENTIAL REFERENCE GENES COMPLETE GENOME SEQUENCE HOUSEKEEPING GENES OLIVE FLOUNDER STREPTOCOCCUS-INIAE EDWARDSIELLA-TARDA EXPRESSION TRANSCRIPTION VALIDATION TISSUES Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1016/j.vetimm.2012.12.018 2022-06-27T05:35:44Z Disease outbreaks caused by iridoviruses are known to affect farmed flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). To facilitate quantitative real time RT-PCR (qRT-PCR) analysis of gene expression in flounder and turbot during viral infection, we in this study examined the potentials of 9 housekeeping genes of flounder and turbot as internal references for qRT-PCR under conditions of experimental infection with megalocytivirus, a member of the Iridoviridae family. The mRNA levels of the 9 housekeeping genes in the brain, gill, heart, intestine, kidney, liver, muscle, and spleen of flounder and turbot were determined by qRT-PCR at 24h and 72 h post-viral infection, and the expression stabilities of the genes were determined with geNorm and NormFinder algorithms. The results showed that (i) viral infection induced significant changes in the mRNA levels of the all the examined genes in a manner that was dependent on both tissue type and infection stage; (ii) for a given time point of infection, stability predictions made by the two algorisms were highly consistent for most tissues; (iii) the optimum reference genes differed at different infection time points at least in some tissues; (iv) at both examined time points, no common reference genes were identified across all tissue types. These results indicate that when studying gene expression in flounder and turbot in relation to viral infection, different internal references may have to be used not only for different tissues but also for different infection stages. (C) 2013 Elsevier B.V. All rights reserved. Disease outbreaks caused by iridoviruses are known to affect farmed flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). To facilitate quantitative real time RT-PCR (qRT-PCR) analysis of gene expression in flounder and turbot during viral infection, we in this study examined the potentials of 9 housekeeping genes of flounder and turbot as internal references for qRT-PCR under conditions of experimental infection with ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Veterinary Immunology and Immunopathology 152 3-4 303 316 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Quantitative Real Time Rt-pcr Reference Gene Housekeeping Gene Paralichthys Olivaceus Scophthalmus Maximus Immunology Veterinary Sciences Science & Technology Life Sciences & Biomedicine POTENTIAL REFERENCE GENES COMPLETE GENOME SEQUENCE HOUSEKEEPING GENES OLIVE FLOUNDER STREPTOCOCCUS-INIAE EDWARDSIELLA-TARDA EXPRESSION TRANSCRIPTION VALIDATION TISSUES |
spellingShingle |
Quantitative Real Time Rt-pcr Reference Gene Housekeeping Gene Paralichthys Olivaceus Scophthalmus Maximus Immunology Veterinary Sciences Science & Technology Life Sciences & Biomedicine POTENTIAL REFERENCE GENES COMPLETE GENOME SEQUENCE HOUSEKEEPING GENES OLIVE FLOUNDER STREPTOCOCCUS-INIAE EDWARDSIELLA-TARDA EXPRESSION TRANSCRIPTION VALIDATION TISSUES Zhang, Jian Hu, Yong-hua Sun, Bo-guang Xiao, Zhi-zhong Sun, Li Sun, L Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection |
topic_facet |
Quantitative Real Time Rt-pcr Reference Gene Housekeeping Gene Paralichthys Olivaceus Scophthalmus Maximus Immunology Veterinary Sciences Science & Technology Life Sciences & Biomedicine POTENTIAL REFERENCE GENES COMPLETE GENOME SEQUENCE HOUSEKEEPING GENES OLIVE FLOUNDER STREPTOCOCCUS-INIAE EDWARDSIELLA-TARDA EXPRESSION TRANSCRIPTION VALIDATION TISSUES |
description |
Disease outbreaks caused by iridoviruses are known to affect farmed flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). To facilitate quantitative real time RT-PCR (qRT-PCR) analysis of gene expression in flounder and turbot during viral infection, we in this study examined the potentials of 9 housekeeping genes of flounder and turbot as internal references for qRT-PCR under conditions of experimental infection with megalocytivirus, a member of the Iridoviridae family. The mRNA levels of the 9 housekeeping genes in the brain, gill, heart, intestine, kidney, liver, muscle, and spleen of flounder and turbot were determined by qRT-PCR at 24h and 72 h post-viral infection, and the expression stabilities of the genes were determined with geNorm and NormFinder algorithms. The results showed that (i) viral infection induced significant changes in the mRNA levels of the all the examined genes in a manner that was dependent on both tissue type and infection stage; (ii) for a given time point of infection, stability predictions made by the two algorisms were highly consistent for most tissues; (iii) the optimum reference genes differed at different infection time points at least in some tissues; (iv) at both examined time points, no common reference genes were identified across all tissue types. These results indicate that when studying gene expression in flounder and turbot in relation to viral infection, different internal references may have to be used not only for different tissues but also for different infection stages. (C) 2013 Elsevier B.V. All rights reserved. Disease outbreaks caused by iridoviruses are known to affect farmed flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). To facilitate quantitative real time RT-PCR (qRT-PCR) analysis of gene expression in flounder and turbot during viral infection, we in this study examined the potentials of 9 housekeeping genes of flounder and turbot as internal references for qRT-PCR under conditions of experimental infection with ... |
format |
Article in Journal/Newspaper |
author |
Zhang, Jian Hu, Yong-hua Sun, Bo-guang Xiao, Zhi-zhong Sun, Li Sun, L |
author_facet |
Zhang, Jian Hu, Yong-hua Sun, Bo-guang Xiao, Zhi-zhong Sun, Li Sun, L |
author_sort |
Zhang, Jian |
title |
Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection |
title_short |
Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection |
title_full |
Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection |
title_fullStr |
Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection |
title_full_unstemmed |
Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection |
title_sort |
selection of normalization factors for quantitative real time rt-pcr studies in japanese flounder (paralichthys olivaceus) and turbot (scophthalmus maximus) under conditions of viral infection |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16627 https://doi.org/10.1016/j.vetimm.2012.12.018 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY Zhang, Jian; Hu, Yong-hua; Sun, Bo-guang; Xiao, Zhi-zhong; Sun, Li.Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2013,152(41702):303-316 http://ir.qdio.ac.cn/handle/337002/16627 doi:10.1016/j.vetimm.2012.12.018 |
op_rights |
6 |
op_doi |
https://doi.org/10.1016/j.vetimm.2012.12.018 |
container_title |
Veterinary Immunology and Immunopathology |
container_volume |
152 |
container_issue |
3-4 |
container_start_page |
303 |
op_container_end_page |
316 |
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1766188872794898432 |