Megalocytivirus-induced proteins of turbot (Scophthalmus maximus): Identification and antiviral potential

• Published in: Journal of Proteomics
• Main Authors: Zhang, Jian, Hu, Yong-hua, Xiao, Zhi-zhong, Sun, Li, Sun, L
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2013
• Subjects: Megalocytivirus; Scophthalmus Maximus; Proteomic; Antiviral; Adjuvant; Biochemistry & Molecular Biology; Biochemical Research Methods; Science & Technology; Life Sciences & Biomedicine; RED-SEA BREAM; FLOUNDER PARALICHTHYS-OLIVACEUS; LARGE YELLOW CROAKER; RECEPTOR-MEDIATED ENDOCYTOSIS; TROUT ONCORHYNCHUS-MYKISS; ANTIGEN-PRESENTING CELLS; COMPLETE GENOME SEQUENCE; IRIDOVIRUS-LIKE AGENT; NATURAL-KILLER-CELLS; IN-VITRO INHIBITION; Scophthalmus maximus; Turbot
• Online Access: http://ir.qdio.ac.cn/handle/337002/16602; https://doi.org/10.1016/j.jprot.2013.07.033

Megalocytivirus is an important fish pathogen with a broad host range that includes turbot. In this study, proteomic analysis was conducted to examine turbot proteins modulated in expression by megalocytivirus infection. Thirty five proteins from spleen were identified to be differentially expressed at 2 days post-viral infection (dpi) and 7 dpi. Three upregulated proteins, i.e. heat shock protein 70 (Hsp70), Mx protein, and natural killer enhancing factor (NKEF), were further analyzed for potential antiviral effect. For this purpose, turbot were administered separately with the plasmids pHsp70, pMx, and pNKEF, which express Hsp70, Mx, and NKEF respectively, before megalocytivirus infection. Viral dissemination and propagation in spleen were subsequently determined. The results showed that the viral loads in fish administered with pNKEF were significantly reduced. To examine the potential of Hsp70, Mx, and NKEF as immunological adjuvant, turbot were immunized with a DNA vaccine in the presence of pHsp70, pMx, or pNKEF. Subsequent analysis showed that the presence of pNKEF and pHsp70, but not pMx, significantly reduced viral infection and enhanced fish survival. Taken together, these results indicate that NKEF exhibits antiviral property against megalocytivirus, and that both NKEF and Hsp70 may be used in DNA vaccine-based control of megalocytivirus infection. Megalocytivirus is an important fish pathogen with a broad host range that includes turbot. In this study, proteomic analysis was conducted to examine turbot proteins modulated in expression by megalocytivirus infection. Thirty five proteins from spleen were identified to be differentially expressed at 2 days post-viral infection (dpi) and 7 dpi. Three upregulated proteins, i.e. heat shock protein 70 (Hsp70), Mx protein, and natural killer enhancing factor (NKEF), were further analyzed for potential antiviral effect. For this purpose, turbot were administered separately with the plasmids pHsp70, pMx, and pNKEF, which express Hsp70, Mx, and NKEF ...