Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment
A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 ba...
Published in: | Journal of Invertebrate Pathology |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2013
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/16586 https://doi.org/10.1016/j.jip.2013.07.005 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16586 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16586 2023-05-15T15:59:07+02:00 Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment Liu, Rui Qiu, Limei Yu, Zuoan Zi, Jun Yue, Feng Wang, Lingling Zhang, Huan Teng, Weiming Liu, Xiangfeng Song, Linsheng Song, LS 2013-10-01 http://ir.qdio.ac.cn/handle/337002/16586 https://doi.org/10.1016/j.jip.2013.07.005 英语 eng JOURNAL OF INVERTEBRATE PATHOLOGY Liu, Rui; Qiu, Limei; Yu, Zuoan; Zi, Jun; Yue, Feng; Wang, Lingling; Zhang, Huan; Teng, Weiming; Liu, Xiangfeng; Song, Linsheng.Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment,JOURNAL OF INVERTEBRATE PATHOLOGY,2013,114(2):144-150 http://ir.qdio.ac.cn/handle/337002/16586 doi:10.1016/j.jip.2013.07.005 6 Pathogencity Bacterial Infection Bivalve Disease Lesion Low Temperature Zoology Science & Technology Life Sciences & Biomedicine BROWN RING DISEASE CLAM RUDITAPES-PHILIPPINARUM CRASSOSTREA-GIGAS PACIFIC OYSTER SHELLFISH BACTERIA TAPETIS ALGINOLYTICUS MORTALITIES RESPONSES Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1016/j.jip.2013.07.005 2022-06-27T05:35:40Z A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved. A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Journal of Invertebrate Pathology 114 2 144 150 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pathogencity Bacterial Infection Bivalve Disease Lesion Low Temperature Zoology Science & Technology Life Sciences & Biomedicine BROWN RING DISEASE CLAM RUDITAPES-PHILIPPINARUM CRASSOSTREA-GIGAS PACIFIC OYSTER SHELLFISH BACTERIA TAPETIS ALGINOLYTICUS MORTALITIES RESPONSES |
spellingShingle |
Pathogencity Bacterial Infection Bivalve Disease Lesion Low Temperature Zoology Science & Technology Life Sciences & Biomedicine BROWN RING DISEASE CLAM RUDITAPES-PHILIPPINARUM CRASSOSTREA-GIGAS PACIFIC OYSTER SHELLFISH BACTERIA TAPETIS ALGINOLYTICUS MORTALITIES RESPONSES Liu, Rui Qiu, Limei Yu, Zuoan Zi, Jun Yue, Feng Wang, Lingling Zhang, Huan Teng, Weiming Liu, Xiangfeng Song, Linsheng Song, LS Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment |
topic_facet |
Pathogencity Bacterial Infection Bivalve Disease Lesion Low Temperature Zoology Science & Technology Life Sciences & Biomedicine BROWN RING DISEASE CLAM RUDITAPES-PHILIPPINARUM CRASSOSTREA-GIGAS PACIFIC OYSTER SHELLFISH BACTERIA TAPETIS ALGINOLYTICUS MORTALITIES RESPONSES |
description |
A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved. A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 ... |
format |
Article in Journal/Newspaper |
author |
Liu, Rui Qiu, Limei Yu, Zuoan Zi, Jun Yue, Feng Wang, Lingling Zhang, Huan Teng, Weiming Liu, Xiangfeng Song, Linsheng Song, LS |
author_facet |
Liu, Rui Qiu, Limei Yu, Zuoan Zi, Jun Yue, Feng Wang, Lingling Zhang, Huan Teng, Weiming Liu, Xiangfeng Song, Linsheng Song, LS |
author_sort |
Liu, Rui |
title |
Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment |
title_short |
Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment |
title_full |
Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment |
title_fullStr |
Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment |
title_full_unstemmed |
Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment |
title_sort |
identification and characterisation of pathogenic vibrio splendidus from yesso scallop (patinopecten yessoensis) cultured in a low temperature environment |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16586 https://doi.org/10.1016/j.jip.2013.07.005 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
JOURNAL OF INVERTEBRATE PATHOLOGY Liu, Rui; Qiu, Limei; Yu, Zuoan; Zi, Jun; Yue, Feng; Wang, Lingling; Zhang, Huan; Teng, Weiming; Liu, Xiangfeng; Song, Linsheng.Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment,JOURNAL OF INVERTEBRATE PATHOLOGY,2013,114(2):144-150 http://ir.qdio.ac.cn/handle/337002/16586 doi:10.1016/j.jip.2013.07.005 |
op_rights |
6 |
op_doi |
https://doi.org/10.1016/j.jip.2013.07.005 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
114 |
container_issue |
2 |
container_start_page |
144 |
op_container_end_page |
150 |
_version_ |
1766394908776595456 |