Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides

The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa...

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Published in:Genetics and Molecular Research
Main Authors: Bao, Y. B., Li, L., Ye, M. X., Dong, Y. H., Jin, W. X., Lin, Z. H., Lin, ZH
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
Online Access:http://ir.qdio.ac.cn/handle/337002/16570
https://doi.org/10.4238/2013.April.10.9
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spelling ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16570 2023-05-15T15:59:10+02:00 Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides Bao, Y. B. Li, L. Ye, M. X. Dong, Y. H. Jin, W. X. Lin, Z. H. Lin, ZH 2013 http://ir.qdio.ac.cn/handle/337002/16570 https://doi.org/10.4238/2013.April.10.9 英语 eng GENETICS AND MOLECULAR RESEARCH Bao, Y. B.; Li, L.; Ye, M. X.; Dong, Y. H.; Jin, W. X.; Lin, Z. H.Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides,GENETICS AND MOLECULAR RESEARCH,2013,12(2):1143-1154 http://ir.qdio.ac.cn/handle/337002/16570 doi:10.4238/2013.April.10.9 6 Vibrio Parahaemolyticus Messenger Rna Expression Immune Response Tegillarca Granosa Glutamine Synthetase Biochemistry & Molecular Biology Genetics & Heredity Science & Technology Life Sciences & Biomedicine EVOLUTIONARY RELATIONSHIPS GENE CLONING CDNA ENCODE Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.4238/2013.April.10.9 2022-06-27T05:35:40Z The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up- regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration. The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Genetics and Molecular Research 12 2 1143 1154
institution Open Polar
collection Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR
op_collection_id ftchinacasciocas
language English
topic Vibrio Parahaemolyticus
Messenger Rna Expression
Immune Response
Tegillarca Granosa
Glutamine Synthetase
Biochemistry & Molecular Biology
Genetics & Heredity
Science & Technology
Life Sciences & Biomedicine
EVOLUTIONARY RELATIONSHIPS
GENE
CLONING
CDNA
ENCODE
spellingShingle Vibrio Parahaemolyticus
Messenger Rna Expression
Immune Response
Tegillarca Granosa
Glutamine Synthetase
Biochemistry & Molecular Biology
Genetics & Heredity
Science & Technology
Life Sciences & Biomedicine
EVOLUTIONARY RELATIONSHIPS
GENE
CLONING
CDNA
ENCODE
Bao, Y. B.
Li, L.
Ye, M. X.
Dong, Y. H.
Jin, W. X.
Lin, Z. H.
Lin, ZH
Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
topic_facet Vibrio Parahaemolyticus
Messenger Rna Expression
Immune Response
Tegillarca Granosa
Glutamine Synthetase
Biochemistry & Molecular Biology
Genetics & Heredity
Science & Technology
Life Sciences & Biomedicine
EVOLUTIONARY RELATIONSHIPS
GENE
CLONING
CDNA
ENCODE
description The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up- regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration. The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with ...
format Article in Journal/Newspaper
author Bao, Y. B.
Li, L.
Ye, M. X.
Dong, Y. H.
Jin, W. X.
Lin, Z. H.
Lin, ZH
author_facet Bao, Y. B.
Li, L.
Ye, M. X.
Dong, Y. H.
Jin, W. X.
Lin, Z. H.
Lin, ZH
author_sort Bao, Y. B.
title Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
title_short Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
title_full Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
title_fullStr Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
title_full_unstemmed Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
title_sort expression of glutamine synthetase in tegillarca granosa (bivalvia, arcidae) hemocytes stimulated by vibrio parahaemolyticus and lipopolysaccharides
publishDate 2013
url http://ir.qdio.ac.cn/handle/337002/16570
https://doi.org/10.4238/2013.April.10.9
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_relation GENETICS AND MOLECULAR RESEARCH
Bao, Y. B.; Li, L.; Ye, M. X.; Dong, Y. H.; Jin, W. X.; Lin, Z. H.Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides,GENETICS AND MOLECULAR RESEARCH,2013,12(2):1143-1154
http://ir.qdio.ac.cn/handle/337002/16570
doi:10.4238/2013.April.10.9
op_rights 6
op_doi https://doi.org/10.4238/2013.April.10.9
container_title Genetics and Molecular Research
container_volume 12
container_issue 2
container_start_page 1143
op_container_end_page 1154
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