Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides

• Published in: Genetics and Molecular Research
• Main Authors: Bao, Y. B., Li, L., Ye, M. X., Dong, Y. H., Jin, W. X., Lin, Z. H., Lin, ZH
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2013
• Subjects: Vibrio Parahaemolyticus; Messenger Rna Expression; Immune Response; Tegillarca Granosa; Glutamine Synthetase; Biochemistry & Molecular Biology; Genetics & Heredity; Science & Technology; Life Sciences & Biomedicine; EVOLUTIONARY RELATIONSHIPS; GENE; CLONING; CDNA; ENCODE; Pacific; Crassostrea gigas; Pacific oyster
• Online Access: http://ir.qdio.ac.cn/handle/337002/16570; https://doi.org/10.4238/2013.April.10.9

The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up- regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration. The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase ( GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa ( Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends ( RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with ...