Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum
Edwardsiella tarda and Vibrio anguillarum are severe fish pathogens. In this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of E. tarda-and V. anguillarum-associated diseases. For this purpose, we selected and analyzed 2 seawater isolates,...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16565 2023-05-15T18:15:54+02:00 Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum Wang, Chong Hu, Yong-hua Sun, Bo-guang Chi, Heng Li, Jun Sun, Li Sun, L 2013-03-13 http://ir.qdio.ac.cn/handle/337002/16565 https://doi.org/10.3354/dao02569 英语 eng DISEASES OF AQUATIC ORGANISMS Wang, Chong; Hu, Yong-hua; Sun, Bo-guang; Chi, Heng; Li, Jun; Sun, Li.Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum,DISEASES OF AQUATIC ORGANISMS,2013,103(1):45-53 http://ir.qdio.ac.cn/handle/337002/16565 doi:10.3354/dao02569 6 Bivalent Vaccine Live Vaccine Natural Delivery Cross-protection Fisheries Veterinary Sciences Science & Technology Life Sciences & Biomedicine OUTER-MEMBRANE PROTEINS FISH INFECTION STRAIN MECHANISMS EXPRESSION ANTIGEN Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.3354/dao02569 2022-06-27T05:35:40Z Edwardsiella tarda and Vibrio anguillarum are severe fish pathogens. In this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of E. tarda-and V. anguillarum-associated diseases. For this purpose, we selected and analyzed 2 seawater isolates, P1SW and V3SW, belonging to the genera Pseudomonas and Vibrio, respectively. When administered to turbot Scophthalmus maximus via immersion and oral feeding, P1SW and V3SW at a dose of 2 x 10(8) colony-forming units caused no mortality, but both strains were able to disseminate into internal organs in a transient, time-dependent manner. When turbot were immunized with P1SW, V3SW, or P1SW plus V3SW (named P1V3) via immersion plus oral routes, the latter with vaccines embedded in sodium alginate microspheres, moderate protection against E. tarda and V. anguillarum was induced by V3SW, and moderate protection against E. tarda was induced by P1SW. Compared to P1SW and V3SW, P1V3 elicited a significantly stronger protection against both E. tarda and V. anguillarum. Immunological analysis showed that (1) P1SW, V3SW, and especially P1V3 activated head kidney macrophages, (2) P1V3 induced significantly higher levels of serum antibodies against E. tarda and V. anguillarum than P1SW and V3SW, and (3) P1V3-induced antibodies were able to bind E. tarda and V. anguillarum and enhance serum bactericidal activity. These results indicate that P1V3 as a naturally delivered vaccine elicited a humoral immune response against both E. tarda and V. anguillarum and, as a result, was cross-protective against E. tarda and V. anguillarum infection. Edwardsiella tarda and Vibrio anguillarum are severe fish pathogens. In this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of E. tarda-and V. anguillarum-associated diseases. For this purpose, we selected and analyzed 2 seawater isolates, P1SW and V3SW, belonging to the genera Pseudomonas and Vibrio, respectively. When administered to ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Diseases of Aquatic Organisms 103 1 45 53 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Bivalent Vaccine Live Vaccine Natural Delivery Cross-protection Fisheries Veterinary Sciences Science & Technology Life Sciences & Biomedicine OUTER-MEMBRANE PROTEINS FISH INFECTION STRAIN MECHANISMS EXPRESSION ANTIGEN |
spellingShingle |
Bivalent Vaccine Live Vaccine Natural Delivery Cross-protection Fisheries Veterinary Sciences Science & Technology Life Sciences & Biomedicine OUTER-MEMBRANE PROTEINS FISH INFECTION STRAIN MECHANISMS EXPRESSION ANTIGEN Wang, Chong Hu, Yong-hua Sun, Bo-guang Chi, Heng Li, Jun Sun, Li Sun, L Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum |
topic_facet |
Bivalent Vaccine Live Vaccine Natural Delivery Cross-protection Fisheries Veterinary Sciences Science & Technology Life Sciences & Biomedicine OUTER-MEMBRANE PROTEINS FISH INFECTION STRAIN MECHANISMS EXPRESSION ANTIGEN |
description |
Edwardsiella tarda and Vibrio anguillarum are severe fish pathogens. In this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of E. tarda-and V. anguillarum-associated diseases. For this purpose, we selected and analyzed 2 seawater isolates, P1SW and V3SW, belonging to the genera Pseudomonas and Vibrio, respectively. When administered to turbot Scophthalmus maximus via immersion and oral feeding, P1SW and V3SW at a dose of 2 x 10(8) colony-forming units caused no mortality, but both strains were able to disseminate into internal organs in a transient, time-dependent manner. When turbot were immunized with P1SW, V3SW, or P1SW plus V3SW (named P1V3) via immersion plus oral routes, the latter with vaccines embedded in sodium alginate microspheres, moderate protection against E. tarda and V. anguillarum was induced by V3SW, and moderate protection against E. tarda was induced by P1SW. Compared to P1SW and V3SW, P1V3 elicited a significantly stronger protection against both E. tarda and V. anguillarum. Immunological analysis showed that (1) P1SW, V3SW, and especially P1V3 activated head kidney macrophages, (2) P1V3 induced significantly higher levels of serum antibodies against E. tarda and V. anguillarum than P1SW and V3SW, and (3) P1V3-induced antibodies were able to bind E. tarda and V. anguillarum and enhance serum bactericidal activity. These results indicate that P1V3 as a naturally delivered vaccine elicited a humoral immune response against both E. tarda and V. anguillarum and, as a result, was cross-protective against E. tarda and V. anguillarum infection. Edwardsiella tarda and Vibrio anguillarum are severe fish pathogens. In this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of E. tarda-and V. anguillarum-associated diseases. For this purpose, we selected and analyzed 2 seawater isolates, P1SW and V3SW, belonging to the genera Pseudomonas and Vibrio, respectively. When administered to ... |
format |
Article in Journal/Newspaper |
author |
Wang, Chong Hu, Yong-hua Sun, Bo-guang Chi, Heng Li, Jun Sun, Li Sun, L |
author_facet |
Wang, Chong Hu, Yong-hua Sun, Bo-guang Chi, Heng Li, Jun Sun, Li Sun, L |
author_sort |
Wang, Chong |
title |
Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum |
title_short |
Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum |
title_full |
Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum |
title_fullStr |
Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum |
title_full_unstemmed |
Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum |
title_sort |
environmental isolates p1sw and v3sw as a bivalent vaccine induce effective cross-protection against edwardsiella tarda and vibrio anguillarum |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16565 https://doi.org/10.3354/dao02569 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
DISEASES OF AQUATIC ORGANISMS Wang, Chong; Hu, Yong-hua; Sun, Bo-guang; Chi, Heng; Li, Jun; Sun, Li.Environmental isolates P1SW and V3SW as a bivalent vaccine induce effective cross-protection against Edwardsiella tarda and Vibrio anguillarum,DISEASES OF AQUATIC ORGANISMS,2013,103(1):45-53 http://ir.qdio.ac.cn/handle/337002/16565 doi:10.3354/dao02569 |
op_rights |
6 |
op_doi |
https://doi.org/10.3354/dao02569 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
103 |
container_issue |
1 |
container_start_page |
45 |
op_container_end_page |
53 |
_version_ |
1766189145274712064 |