Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue
Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range that includes fish and humans. In this study, we examined the activity and function of the lysozyme inhibitor Ivy (named Ivy(Et)) identified in the pathogenic E. tarda strain TX01. Ivy(Et) possesses the Ivy signature mo...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16562 2023-05-15T18:15:54+02:00 Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue Wang, Chong Hu, Yong-hua Sun, Bo-guang Li, Jun Sun, Li Sun, L 2013-10-01 http://ir.qdio.ac.cn/handle/337002/16562 https://doi.org/10.1128/IAI.00503-13 英语 eng INFECTION AND IMMUNITY Wang, Chong; Hu, Yong-hua; Sun, Bo-guang; Li, Jun; Sun, Li.Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue,INFECTION AND IMMUNITY,2013,81(10):3527-3533 http://ir.qdio.ac.cn/handle/337002/16562 doi:10.1128/IAI.00503-13 6 Immunology Infectious Diseases Science & Technology Life Sciences & Biomedicine ESCHERICHIA-COLI RESISTANCE FISH MECHANISMS VIRULENCE SURVIVAL BACTERIA Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1128/IAI.00503-13 2022-06-27T05:35:40Z Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range that includes fish and humans. In this study, we examined the activity and function of the lysozyme inhibitor Ivy (named Ivy(Et)) identified in the pathogenic E. tarda strain TX01. Ivy(Et) possesses the Ivy signature motif CKPHDC in the form of (82)CQPHNC(87) and contains several highly conserved residues, including a tryptophan (W55). For the purpose of virulence analysis, an isogenic TX01 mutant, TXivy, was created. TXivy bears an in-frame deletion of the ivy(Et) gene. A live infection study in a turbot (Scophthalmus maximus) model showed that, compared to TX01, TXivy exhibited attenuated overall virulence, reduced tissue dissemination and colonization capacity, an impaired ability to replicate in host macrophages, and decreased resistance against the bactericidal effect of host serum. To facilitate functional analysis, recombinant Ivy(Et) (rIvy) and three mutant proteins, i.e., rIvyW55A, rIvyC82S, and rIvyH85D, which bear Ala, Ser, and Asp substitutions at W55, C82, and H85, respectively, were prepared. In vitro studies showed that rIvy, rIvyW55A, and rIvyH85D were able to block the lytic effect of lysozyme on a Gram-positive bacterium, whereas rIvyC82S could not do so. Likewise, rIvy, but not rIvyC82S, inhibited the serum-facilitated killing effect of lysozyme on E. tarda. In vivo analysis showed that rIvy, but not rIvyC82S, restored the lost pathogenicity of TXivy and enhanced the infectivity of TX01. Together these results indicate that IvyEt is a lysozyme inhibitor and a virulence factor that depends on the conserved C82 for biological activity. Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range that includes fish and humans. In this study, we examined the activity and function of the lysozyme inhibitor Ivy (named Ivy(Et)) identified in the pathogenic E. tarda strain TX01. Ivy(Et) possesses the Ivy signature motif CKPHDC in the form of (82)CQPHNC(87) and contains several highly conserved ... Article in Journal/Newspaper Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Infection and Immunity 81 10 3527 3533 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Immunology Infectious Diseases Science & Technology Life Sciences & Biomedicine ESCHERICHIA-COLI RESISTANCE FISH MECHANISMS VIRULENCE SURVIVAL BACTERIA |
spellingShingle |
Immunology Infectious Diseases Science & Technology Life Sciences & Biomedicine ESCHERICHIA-COLI RESISTANCE FISH MECHANISMS VIRULENCE SURVIVAL BACTERIA Wang, Chong Hu, Yong-hua Sun, Bo-guang Li, Jun Sun, Li Sun, L Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue |
topic_facet |
Immunology Infectious Diseases Science & Technology Life Sciences & Biomedicine ESCHERICHIA-COLI RESISTANCE FISH MECHANISMS VIRULENCE SURVIVAL BACTERIA |
description |
Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range that includes fish and humans. In this study, we examined the activity and function of the lysozyme inhibitor Ivy (named Ivy(Et)) identified in the pathogenic E. tarda strain TX01. Ivy(Et) possesses the Ivy signature motif CKPHDC in the form of (82)CQPHNC(87) and contains several highly conserved residues, including a tryptophan (W55). For the purpose of virulence analysis, an isogenic TX01 mutant, TXivy, was created. TXivy bears an in-frame deletion of the ivy(Et) gene. A live infection study in a turbot (Scophthalmus maximus) model showed that, compared to TX01, TXivy exhibited attenuated overall virulence, reduced tissue dissemination and colonization capacity, an impaired ability to replicate in host macrophages, and decreased resistance against the bactericidal effect of host serum. To facilitate functional analysis, recombinant Ivy(Et) (rIvy) and three mutant proteins, i.e., rIvyW55A, rIvyC82S, and rIvyH85D, which bear Ala, Ser, and Asp substitutions at W55, C82, and H85, respectively, were prepared. In vitro studies showed that rIvy, rIvyW55A, and rIvyH85D were able to block the lytic effect of lysozyme on a Gram-positive bacterium, whereas rIvyC82S could not do so. Likewise, rIvy, but not rIvyC82S, inhibited the serum-facilitated killing effect of lysozyme on E. tarda. In vivo analysis showed that rIvy, but not rIvyC82S, restored the lost pathogenicity of TXivy and enhanced the infectivity of TX01. Together these results indicate that IvyEt is a lysozyme inhibitor and a virulence factor that depends on the conserved C82 for biological activity. Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range that includes fish and humans. In this study, we examined the activity and function of the lysozyme inhibitor Ivy (named Ivy(Et)) identified in the pathogenic E. tarda strain TX01. Ivy(Et) possesses the Ivy signature motif CKPHDC in the form of (82)CQPHNC(87) and contains several highly conserved ... |
format |
Article in Journal/Newspaper |
author |
Wang, Chong Hu, Yong-hua Sun, Bo-guang Li, Jun Sun, Li Sun, L |
author_facet |
Wang, Chong Hu, Yong-hua Sun, Bo-guang Li, Jun Sun, Li Sun, L |
author_sort |
Wang, Chong |
title |
Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue |
title_short |
Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue |
title_full |
Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue |
title_fullStr |
Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue |
title_full_unstemmed |
Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue |
title_sort |
edwardsiella tarda ivy, a lysozyme inhibitor that blocks the lytic effect of lysozyme and facilitates host infection in a manner that is dependent on the conserved cysteine residue |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16562 https://doi.org/10.1128/IAI.00503-13 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
INFECTION AND IMMUNITY Wang, Chong; Hu, Yong-hua; Sun, Bo-guang; Li, Jun; Sun, Li.Edwardsiella tarda Ivy, a Lysozyme Inhibitor That Blocks the Lytic Effect of Lysozyme and Facilitates Host Infection in a Manner That Is Dependent on the Conserved Cysteine Residue,INFECTION AND IMMUNITY,2013,81(10):3527-3533 http://ir.qdio.ac.cn/handle/337002/16562 doi:10.1128/IAI.00503-13 |
op_rights |
6 |
op_doi |
https://doi.org/10.1128/IAI.00503-13 |
container_title |
Infection and Immunity |
container_volume |
81 |
container_issue |
10 |
container_start_page |
3527 |
op_container_end_page |
3533 |
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1766189145076531200 |