Identification of three cell populations from the shell gland of a bivalve mollusc
The molluscan larval shell formation is a complicated process. There is evidence that the mantle of the primary larva (trochophore) contains functionally different cell populations with distinct gene expression profiles. However, it remains unclear how these cells are specified. In the present study...
Published in: | Development Genes and Evolution |
---|---|
Main Authors: | , , |
Format: | Report |
Language: | English |
Published: |
SPRINGER
2020
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/165390 https://doi.org/10.1007/s00427-020-00646-9 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/165390 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/165390 2023-05-15T15:58:54+02:00 Identification of three cell populations from the shell gland of a bivalve mollusc Liu, Gang Huan, Pin Liu, Baozhong 2020 http://ir.qdio.ac.cn/handle/337002/165390 https://doi.org/10.1007/s00427-020-00646-9 英语 eng SPRINGER DEVELOPMENT GENES AND EVOLUTION http://ir.qdio.ac.cn/handle/337002/165390 doi:10.1007/s00427-020-00646-9 Cell population Mollusc Shell formation gene Shell gland Cell Biology Evolutionary Biology Developmental Biology LARVAL DEVELOPMENT EMBRYONIC-DEVELOPMENT EXPRESSION GENE BOUNDARIES PARAHOX DPP HOX 期刊论文 2020 ftchinacasciocas https://doi.org/10.1007/s00427-020-00646-9 2022-06-27T05:42:06Z The molluscan larval shell formation is a complicated process. There is evidence that the mantle of the primary larva (trochophore) contains functionally different cell populations with distinct gene expression profiles. However, it remains unclear how these cells are specified. In the present study, we identified three cell populations from the shell gland in earlier stages (gastrula) from the bivalve mollusc Crassostrea gigas. These cell populations were determined by analyzing the co-expression relationships among six potential shell formation (pSF) genes using two-color hybridization. The three cell populations, which we designated as SGCPs (shell gland cell populations), formed a concentric-circle pattern from outside to inside of the shell gland. SGCP I was located in the outer edge of the shell gland and the cells expressed pax2/5/8, gata2/3, and bmp2/4. SGCP II was located more internally and the cells expressed two engrailed genes. The last population, SGCP III, was located in the central region of the shell gland and the cells expressed lox4. Determination of the gene expression profiles of SGCPs would help trace their origins and fates and elucidate how these cell populations are specified. Moreover, potential roles of the SGCPs, e.g., development of sensory cells and shell biogenesis, are suggested. Our results reveal the internal organization of the embryonic shell gland at the molecular level and add to the knowledge of larval shell formation. Report Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Development Genes and Evolution 230 1 39 45 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Cell population Mollusc Shell formation gene Shell gland Cell Biology Evolutionary Biology Developmental Biology LARVAL DEVELOPMENT EMBRYONIC-DEVELOPMENT EXPRESSION GENE BOUNDARIES PARAHOX DPP HOX |
spellingShingle |
Cell population Mollusc Shell formation gene Shell gland Cell Biology Evolutionary Biology Developmental Biology LARVAL DEVELOPMENT EMBRYONIC-DEVELOPMENT EXPRESSION GENE BOUNDARIES PARAHOX DPP HOX Liu, Gang Huan, Pin Liu, Baozhong Identification of three cell populations from the shell gland of a bivalve mollusc |
topic_facet |
Cell population Mollusc Shell formation gene Shell gland Cell Biology Evolutionary Biology Developmental Biology LARVAL DEVELOPMENT EMBRYONIC-DEVELOPMENT EXPRESSION GENE BOUNDARIES PARAHOX DPP HOX |
description |
The molluscan larval shell formation is a complicated process. There is evidence that the mantle of the primary larva (trochophore) contains functionally different cell populations with distinct gene expression profiles. However, it remains unclear how these cells are specified. In the present study, we identified three cell populations from the shell gland in earlier stages (gastrula) from the bivalve mollusc Crassostrea gigas. These cell populations were determined by analyzing the co-expression relationships among six potential shell formation (pSF) genes using two-color hybridization. The three cell populations, which we designated as SGCPs (shell gland cell populations), formed a concentric-circle pattern from outside to inside of the shell gland. SGCP I was located in the outer edge of the shell gland and the cells expressed pax2/5/8, gata2/3, and bmp2/4. SGCP II was located more internally and the cells expressed two engrailed genes. The last population, SGCP III, was located in the central region of the shell gland and the cells expressed lox4. Determination of the gene expression profiles of SGCPs would help trace their origins and fates and elucidate how these cell populations are specified. Moreover, potential roles of the SGCPs, e.g., development of sensory cells and shell biogenesis, are suggested. Our results reveal the internal organization of the embryonic shell gland at the molecular level and add to the knowledge of larval shell formation. |
format |
Report |
author |
Liu, Gang Huan, Pin Liu, Baozhong |
author_facet |
Liu, Gang Huan, Pin Liu, Baozhong |
author_sort |
Liu, Gang |
title |
Identification of three cell populations from the shell gland of a bivalve mollusc |
title_short |
Identification of three cell populations from the shell gland of a bivalve mollusc |
title_full |
Identification of three cell populations from the shell gland of a bivalve mollusc |
title_fullStr |
Identification of three cell populations from the shell gland of a bivalve mollusc |
title_full_unstemmed |
Identification of three cell populations from the shell gland of a bivalve mollusc |
title_sort |
identification of three cell populations from the shell gland of a bivalve mollusc |
publisher |
SPRINGER |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/165390 https://doi.org/10.1007/s00427-020-00646-9 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
DEVELOPMENT GENES AND EVOLUTION http://ir.qdio.ac.cn/handle/337002/165390 doi:10.1007/s00427-020-00646-9 |
op_doi |
https://doi.org/10.1007/s00427-020-00646-9 |
container_title |
Development Genes and Evolution |
container_volume |
230 |
container_issue |
1 |
container_start_page |
39 |
op_container_end_page |
45 |
_version_ |
1766394676906033152 |