Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress
Lysine acetylation of proteins is a highly conserved post-translational modification that plays an important regulatory role in almost every aspect of metabolic processes in both terrestrial and aquatic species. Pacific oyster, Crassostrea gigas, a model marine species, is distributed worldwide and...
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Online Access: | http://ir.qdio.ac.cn/handle/337002/165277 http://ir.qdio.ac.cn/handle/337002/165278 https://doi.org/10.1007/s10126-020-09947-6 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/165278 2023-05-15T15:58:17+02:00 Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress Li, Ao Li, Li Wang, Wei Zhang, Guofan 2020-01-29 http://ir.qdio.ac.cn/handle/337002/165277 http://ir.qdio.ac.cn/handle/337002/165278 https://doi.org/10.1007/s10126-020-09947-6 英语 eng SPRINGER MARINE BIOTECHNOLOGY http://ir.qdio.ac.cn/handle/337002/165277 http://ir.qdio.ac.cn/handle/337002/165278 doi:10.1007/s10126-020-09947-6 Acetylome Posttranslational modification Adaptive divergence Oyster Climate change Biotechnology & Applied Microbiology Marine & Freshwater Biology 期刊论文 2020 ftchinacasciocas https://doi.org/10.1007/s10126-020-09947-6 2022-06-27T05:42:06Z Lysine acetylation of proteins is a highly conserved post-translational modification that plays an important regulatory role in almost every aspect of metabolic processes in both terrestrial and aquatic species. Pacific oyster, Crassostrea gigas, a model marine species, is distributed worldwide and is economically and ecologically important. However, little is known about the role of acetylation in the adaptive response of oyster to heterogeneous intertidal environments. Here, we conducted the first-ever lysine acetylome analysis in two genetically and physiologically differentiated oyster populations, using a highly sensitive immune-affinity purification and high-resolution mass spectrometry. Overall, we identified 1054 lysine acetylation sites in 664 proteins, which account for 2.37% of the oyster proteome analysed in the current study. The modified proteins are involved in a wide range of biological processes and are localised in multiple cellular compartments. Motif analysis revealed that hydrophilic and polar amino acids histidine, lysine and arginine were the most enriched residues in the positions + 1 and + 2 of the acetylated sites. Further, the two oyster populations exhibited divergent acetylomic regulations of several biological pathways, particularly energy metabolism and glycine and serine amino acid metabolism, in response to thermal stress and differentiated acetylation patters of candidate heat-responsive proteins, e.g. molecular chaperone and myosin. These observations suggest that lysine acetylation plays a critical role in different thermal responses of these two oyster populations. These findings provide an important resource for in-depth exploration of the physiological role of lysine acetylation in adaptive evolution of marine invertebrates. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Marine Biotechnology 22 2 233 245 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Acetylome Posttranslational modification Adaptive divergence Oyster Climate change Biotechnology & Applied Microbiology Marine & Freshwater Biology |
spellingShingle |
Acetylome Posttranslational modification Adaptive divergence Oyster Climate change Biotechnology & Applied Microbiology Marine & Freshwater Biology Li, Ao Li, Li Wang, Wei Zhang, Guofan Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress |
topic_facet |
Acetylome Posttranslational modification Adaptive divergence Oyster Climate change Biotechnology & Applied Microbiology Marine & Freshwater Biology |
description |
Lysine acetylation of proteins is a highly conserved post-translational modification that plays an important regulatory role in almost every aspect of metabolic processes in both terrestrial and aquatic species. Pacific oyster, Crassostrea gigas, a model marine species, is distributed worldwide and is economically and ecologically important. However, little is known about the role of acetylation in the adaptive response of oyster to heterogeneous intertidal environments. Here, we conducted the first-ever lysine acetylome analysis in two genetically and physiologically differentiated oyster populations, using a highly sensitive immune-affinity purification and high-resolution mass spectrometry. Overall, we identified 1054 lysine acetylation sites in 664 proteins, which account for 2.37% of the oyster proteome analysed in the current study. The modified proteins are involved in a wide range of biological processes and are localised in multiple cellular compartments. Motif analysis revealed that hydrophilic and polar amino acids histidine, lysine and arginine were the most enriched residues in the positions + 1 and + 2 of the acetylated sites. Further, the two oyster populations exhibited divergent acetylomic regulations of several biological pathways, particularly energy metabolism and glycine and serine amino acid metabolism, in response to thermal stress and differentiated acetylation patters of candidate heat-responsive proteins, e.g. molecular chaperone and myosin. These observations suggest that lysine acetylation plays a critical role in different thermal responses of these two oyster populations. These findings provide an important resource for in-depth exploration of the physiological role of lysine acetylation in adaptive evolution of marine invertebrates. |
format |
Report |
author |
Li, Ao Li, Li Wang, Wei Zhang, Guofan |
author_facet |
Li, Ao Li, Li Wang, Wei Zhang, Guofan |
author_sort |
Li, Ao |
title |
Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress |
title_short |
Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress |
title_full |
Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress |
title_fullStr |
Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress |
title_full_unstemmed |
Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress |
title_sort |
acetylome analysis reveals population differentiation of the pacific oyster crassostrea gigas in response to heat stress |
publisher |
SPRINGER |
publishDate |
2020 |
url |
http://ir.qdio.ac.cn/handle/337002/165277 http://ir.qdio.ac.cn/handle/337002/165278 https://doi.org/10.1007/s10126-020-09947-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
MARINE BIOTECHNOLOGY http://ir.qdio.ac.cn/handle/337002/165277 http://ir.qdio.ac.cn/handle/337002/165278 doi:10.1007/s10126-020-09947-6 |
op_doi |
https://doi.org/10.1007/s10126-020-09947-6 |
container_title |
Marine Biotechnology |
container_volume |
22 |
container_issue |
2 |
container_start_page |
233 |
op_container_end_page |
245 |
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1766394007488823296 |