Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis
The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and enc...
Published in: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/16502 https://doi.org/10.1016/j.cbpc.2012.10.002 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/16502 2023-05-15T17:54:21+02:00 Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis Fang, Yan Yang, Hongsheng Liu, Baozhong Zhang, Libing Yang, HS 2013 http://ir.qdio.ac.cn/handle/337002/16502 https://doi.org/10.1016/j.cbpc.2012.10.002 英语 eng COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY Fang, Yan; Yang, Hongsheng; Liu, Baozhong; Zhang, Libing.Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2013,157(1):54-62 http://ir.qdio.ac.cn/handle/337002/16502 doi:10.1016/j.cbpc.2012.10.002 6 Clam Mollusk Lysozyme Metallothionein Superoxide Dismutase Heavy Metals Bacteria Mactra Veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Science & Technology Life Sciences & Biomedicine OYSTER CRASSOSTREA-VIRGINICA PACIFIC OYSTER MESSENGER-RNA MYTILUS-GALLOPROVINCIALIS MEDITERRANEAN MUSSEL DREISSENA-POLYMORPHA CERASTODERMA-EDULE CADMIUM EXPOSURE GENE-EXPRESSION CDNA CLONING Article 期刊论文 2013 ftchinacasciocas https://doi.org/10.1016/j.cbpc.2012.10.002 2022-06-27T05:35:36Z The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or in combination, for 7 days. Cumulative mortality of clams and mRNA expressions of the three defense components were analyzed. The highest cumulative mortality took place in the combined treatment on day 7. The expression of the three genes was up-regulated in response to treatments compared to the control with different response times and transcriptional levels; the response to combined exposure occurred earlier than to single exposure. Among the experimental groups. MvLYZ expression and MvSOD expression peaked in the combined treatment on day 3, whereas MvMT expression peaked in heavy metals treatment on day 5. Furthermore, interactive effects of heavy metals and Vibrio on transcriptional response changed over the exposure time. Therefore, transcriptional regulation of the three genes under combined exposure was more complex than under single exposure. (C) 2012 Elsevier Inc. All rights reserved. The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or ... Article in Journal/Newspaper Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 157 1 54 62 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Clam Mollusk Lysozyme Metallothionein Superoxide Dismutase Heavy Metals Bacteria Mactra Veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Science & Technology Life Sciences & Biomedicine OYSTER CRASSOSTREA-VIRGINICA PACIFIC OYSTER MESSENGER-RNA MYTILUS-GALLOPROVINCIALIS MEDITERRANEAN MUSSEL DREISSENA-POLYMORPHA CERASTODERMA-EDULE CADMIUM EXPOSURE GENE-EXPRESSION CDNA CLONING |
spellingShingle |
Clam Mollusk Lysozyme Metallothionein Superoxide Dismutase Heavy Metals Bacteria Mactra Veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Science & Technology Life Sciences & Biomedicine OYSTER CRASSOSTREA-VIRGINICA PACIFIC OYSTER MESSENGER-RNA MYTILUS-GALLOPROVINCIALIS MEDITERRANEAN MUSSEL DREISSENA-POLYMORPHA CERASTODERMA-EDULE CADMIUM EXPOSURE GENE-EXPRESSION CDNA CLONING Fang, Yan Yang, Hongsheng Liu, Baozhong Zhang, Libing Yang, HS Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis |
topic_facet |
Clam Mollusk Lysozyme Metallothionein Superoxide Dismutase Heavy Metals Bacteria Mactra Veneriformis Biochemistry & Molecular Biology Endocrinology & Metabolism Toxicology Zoology Science & Technology Life Sciences & Biomedicine OYSTER CRASSOSTREA-VIRGINICA PACIFIC OYSTER MESSENGER-RNA MYTILUS-GALLOPROVINCIALIS MEDITERRANEAN MUSSEL DREISSENA-POLYMORPHA CERASTODERMA-EDULE CADMIUM EXPOSURE GENE-EXPRESSION CDNA CLONING |
description |
The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or in combination, for 7 days. Cumulative mortality of clams and mRNA expressions of the three defense components were analyzed. The highest cumulative mortality took place in the combined treatment on day 7. The expression of the three genes was up-regulated in response to treatments compared to the control with different response times and transcriptional levels; the response to combined exposure occurred earlier than to single exposure. Among the experimental groups. MvLYZ expression and MvSOD expression peaked in the combined treatment on day 3, whereas MvMT expression peaked in heavy metals treatment on day 5. Furthermore, interactive effects of heavy metals and Vibrio on transcriptional response changed over the exposure time. Therefore, transcriptional regulation of the three genes under combined exposure was more complex than under single exposure. (C) 2012 Elsevier Inc. All rights reserved. The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or ... |
format |
Article in Journal/Newspaper |
author |
Fang, Yan Yang, Hongsheng Liu, Baozhong Zhang, Libing Yang, HS |
author_facet |
Fang, Yan Yang, Hongsheng Liu, Baozhong Zhang, Libing Yang, HS |
author_sort |
Fang, Yan |
title |
Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis |
title_short |
Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis |
title_full |
Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis |
title_fullStr |
Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis |
title_full_unstemmed |
Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis |
title_sort |
transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in mactra veneriformis |
publishDate |
2013 |
url |
http://ir.qdio.ac.cn/handle/337002/16502 https://doi.org/10.1016/j.cbpc.2012.10.002 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY Fang, Yan; Yang, Hongsheng; Liu, Baozhong; Zhang, Libing.Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis,COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2013,157(1):54-62 http://ir.qdio.ac.cn/handle/337002/16502 doi:10.1016/j.cbpc.2012.10.002 |
op_rights |
6 |
op_doi |
https://doi.org/10.1016/j.cbpc.2012.10.002 |
container_title |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
container_volume |
157 |
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1 |
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54 |
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62 |
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1766162115258744832 |