Transcriptional response of lysozyme, metallothionein, and superoxide dismutase to combined exposure to heavy metals and bacteria in Mactra veneriformis

The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and enc...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Main Authors: Fang, Yan, Yang, Hongsheng, Liu, Baozhong, Zhang, Libing, Yang, HS
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
Clam
Mollusk
Lysozyme
Metallothionein
Superoxide Dismutase
Heavy Metals
Bacteria
Mactra Veneriformis
Biochemistry & Molecular Biology
Endocrinology & Metabolism
Toxicology
Zoology
Science & Technology
Life Sciences & Biomedicine
OYSTER CRASSOSTREA-VIRGINICA
PACIFIC OYSTER
MESSENGER-RNA
MYTILUS-GALLOPROVINCIALIS
MEDITERRANEAN MUSSEL
DREISSENA-POLYMORPHA
CERASTODERMA-EDULE
CADMIUM EXPOSURE
GENE-EXPRESSION
CDNA CLONING
Pacific
Pacific oyster
Online Access:http://ir.qdio.ac.cn/handle/337002/16502
https://doi.org/10.1016/j.cbpc.2012.10.002
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Summary:The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or in combination, for 7 days. Cumulative mortality of clams and mRNA expressions of the three defense components were analyzed. The highest cumulative mortality took place in the combined treatment on day 7. The expression of the three genes was up-regulated in response to treatments compared to the control with different response times and transcriptional levels; the response to combined exposure occurred earlier than to single exposure. Among the experimental groups. MvLYZ expression and MvSOD expression peaked in the combined treatment on day 3, whereas MvMT expression peaked in heavy metals treatment on day 5. Furthermore, interactive effects of heavy metals and Vibrio on transcriptional response changed over the exposure time. Therefore, transcriptional regulation of the three genes under combined exposure was more complex than under single exposure. (C) 2012 Elsevier Inc. All rights reserved. The response of the defense components lysozyme (LYZ), metallothionein (MT), and superoxide dismutase (SOD) to combined exposure to heavy metals and bacteria was assessed at transcriptional level in the surf clam Mactra veneriformis. First, the full-length LYZ cDNA containing 808 nucleotides and encoding 194 deduced amino acids was identified from the clam. Multiple alignments revealed that MvLYZ had a high identity with invertebrate-type LYZs from other mollusks. Next, clams were exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, alone or ...

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