bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas
The basic helix-loop-helix (bHLH) genes encode a large superfamily of transcription factors in the Pacific oyster (Crassostrea gigas), and play a very important role in regulation of growth and development. To investigate the oyster growth traits and the associations with bHLH genes variations, we a...
Published in: | Journal of Oceanology and Limnology |
---|---|
Main Authors: | , , , , , , , |
Format: | Report |
Language: | English |
Published: |
SCIENCE PRESS
2019
|
Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/163851 https://doi.org/10.1007/s00343-019-9070-4 |
id |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/163851 |
---|---|
record_format |
openpolar |
spelling |
ftchinacasciocas:oai:ir.qdio.ac.cn:337002/163851 2023-05-15T15:57:37+02:00 bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas Chen Na Li Li Li Chenghua Lin Zhihua Meng Jie Liu Sheng Song Kai Bao Yongbo 2019-12-05 http://ir.qdio.ac.cn/handle/337002/163851 https://doi.org/10.1007/s00343-019-9070-4 英语 eng SCIENCE PRESS JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.qdio.ac.cn/handle/337002/163851 doi:10.1007/s00343-019-9070-4 Crassostrea gigas basic helix-loop-helix (bHLH) re-sequencing single nucleotide polymorphism (SNP) shot growth traits Marine & Freshwater Biology Oceanography Limnology CLASSIFICATION ADAPTATION EVOLUTION GENETICS 期刊论文 2019 ftchinacasciocas https://doi.org/10.1007/s00343-019-9070-4 2022-06-27T05:41:32Z The basic helix-loop-helix (bHLH) genes encode a large superfamily of transcription factors in the Pacific oyster (Crassostrea gigas), and play a very important role in regulation of growth and development. To investigate the oyster growth traits and the associations with bHLH genes variations, we analyzed the gene polymorphisms-traits association in a wild population, and confirmed the results in another independent wild population by targeted gene re-sequencing and SNPshot analysis. After screening the single nucleotide polymorphisms (SNPs) in three candidate genes of the bHLH family (88 bHLH genes in two wild oyster populations in total), we identified the allele CgLoblHLH4-T/G located in the exon of the CgLoblHLH4 gene. This allele is a non-synonymous mutation (Phe/Leu) with an extremely significant association with shell width (P<0.01) and allele G is beneficial to shell width. This SNP on the CgLoblHLH4 gene might have a potential value as a genetic marker of growth traits that could be used in breeding in C. gigas in the future. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Journal of Oceanology and Limnology 38 3 862 868 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Crassostrea gigas basic helix-loop-helix (bHLH) re-sequencing single nucleotide polymorphism (SNP) shot growth traits Marine & Freshwater Biology Oceanography Limnology CLASSIFICATION ADAPTATION EVOLUTION GENETICS |
spellingShingle |
Crassostrea gigas basic helix-loop-helix (bHLH) re-sequencing single nucleotide polymorphism (SNP) shot growth traits Marine & Freshwater Biology Oceanography Limnology CLASSIFICATION ADAPTATION EVOLUTION GENETICS Chen Na Li Li Li Chenghua Lin Zhihua Meng Jie Liu Sheng Song Kai Bao Yongbo bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas basic helix-loop-helix (bHLH) re-sequencing single nucleotide polymorphism (SNP) shot growth traits Marine & Freshwater Biology Oceanography Limnology CLASSIFICATION ADAPTATION EVOLUTION GENETICS |
description |
The basic helix-loop-helix (bHLH) genes encode a large superfamily of transcription factors in the Pacific oyster (Crassostrea gigas), and play a very important role in regulation of growth and development. To investigate the oyster growth traits and the associations with bHLH genes variations, we analyzed the gene polymorphisms-traits association in a wild population, and confirmed the results in another independent wild population by targeted gene re-sequencing and SNPshot analysis. After screening the single nucleotide polymorphisms (SNPs) in three candidate genes of the bHLH family (88 bHLH genes in two wild oyster populations in total), we identified the allele CgLoblHLH4-T/G located in the exon of the CgLoblHLH4 gene. This allele is a non-synonymous mutation (Phe/Leu) with an extremely significant association with shell width (P<0.01) and allele G is beneficial to shell width. This SNP on the CgLoblHLH4 gene might have a potential value as a genetic marker of growth traits that could be used in breeding in C. gigas in the future. |
format |
Report |
author |
Chen Na Li Li Li Chenghua Lin Zhihua Meng Jie Liu Sheng Song Kai Bao Yongbo |
author_facet |
Chen Na Li Li Li Chenghua Lin Zhihua Meng Jie Liu Sheng Song Kai Bao Yongbo |
author_sort |
Chen Na |
title |
bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas |
title_short |
bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas |
title_full |
bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas |
title_fullStr |
bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas |
title_sort |
bhlh genes polymorphisms and their association with growth traits in the pacific oyster crassostrea gigas |
publisher |
SCIENCE PRESS |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/163851 https://doi.org/10.1007/s00343-019-9070-4 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.qdio.ac.cn/handle/337002/163851 doi:10.1007/s00343-019-9070-4 |
op_doi |
https://doi.org/10.1007/s00343-019-9070-4 |
container_title |
Journal of Oceanology and Limnology |
container_volume |
38 |
container_issue |
3 |
container_start_page |
862 |
op_container_end_page |
868 |
_version_ |
1766393289191194624 |