T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis
Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/163411 2023-05-15T15:26:55+02:00 T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis Chi, Heng Sormo, Kristian Gillebo Diao, Jing Dalmo, Roy Ambli 2019-10-01 http://ir.qdio.ac.cn/handle/337002/163410 http://ir.qdio.ac.cn/handle/337002/163411 https://doi.org/10.1016/j.fsi.2019.07.021 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/163410 http://ir.qdio.ac.cn/handle/337002/163411 doi:10.1016/j.fsi.2019.07.021 Eomesodermin Atlantic cod Molecular cloning Promoter structure Function analysis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences 期刊论文 2019 ftchinacasciocas https://doi.org/10.1016/j.fsi.2019.07.021 2022-06-27T05:41:21Z Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which possesses a TBOX_3 domain similar to its counterpart in mammals. The regulated expression was observed in head kidney and spleen in response to live Vibrio anguillarum infection in vivo, and spleen leukocytes in vitro after PMA and poly I:C stimulation. Furthermore, we determined a 694 bp sequence, upstream of the transcriptional start site (TSS), to contain a number of sequence motifs that matched known transcription factor-binding sites, Activities of the presumptive regulatory gene were assessed by transfecting different 5'-deletion constructs in CHSE-214 cells. The results showed that the basal promoters and positive transcriptional regulator activities of GmEomes were dependent by sequences located from -694 to -376 bp upstream of TSS. Furthermore, we found that some Eomes binding sites were present in the 5'-flanking regions of the cod IFN gamma gene predicted by bioinformatics. However, Co-transfection of eomesodermin overexpression plasmids with INF gamma reporter vector into CHSE-214 cells determined that Atlantic cod eomesodermin played a minor role in activation of the INF gamma promoter. Report atlantic cod Gadus morhua Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 93 28 38 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Eomesodermin Atlantic cod Molecular cloning Promoter structure Function analysis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Eomesodermin Atlantic cod Molecular cloning Promoter structure Function analysis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Chi, Heng Sormo, Kristian Gillebo Diao, Jing Dalmo, Roy Ambli T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis |
topic_facet |
Eomesodermin Atlantic cod Molecular cloning Promoter structure Function analysis Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which possesses a TBOX_3 domain similar to its counterpart in mammals. The regulated expression was observed in head kidney and spleen in response to live Vibrio anguillarum infection in vivo, and spleen leukocytes in vitro after PMA and poly I:C stimulation. Furthermore, we determined a 694 bp sequence, upstream of the transcriptional start site (TSS), to contain a number of sequence motifs that matched known transcription factor-binding sites, Activities of the presumptive regulatory gene were assessed by transfecting different 5'-deletion constructs in CHSE-214 cells. The results showed that the basal promoters and positive transcriptional regulator activities of GmEomes were dependent by sequences located from -694 to -376 bp upstream of TSS. Furthermore, we found that some Eomes binding sites were present in the 5'-flanking regions of the cod IFN gamma gene predicted by bioinformatics. However, Co-transfection of eomesodermin overexpression plasmids with INF gamma reporter vector into CHSE-214 cells determined that Atlantic cod eomesodermin played a minor role in activation of the INF gamma promoter. |
format |
Report |
author |
Chi, Heng Sormo, Kristian Gillebo Diao, Jing Dalmo, Roy Ambli |
author_facet |
Chi, Heng Sormo, Kristian Gillebo Diao, Jing Dalmo, Roy Ambli |
author_sort |
Chi, Heng |
title |
T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis |
title_short |
T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis |
title_full |
T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis |
title_fullStr |
T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis |
title_full_unstemmed |
T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis |
title_sort |
t-box transcription factor eomesodermin/tbr2 in atlantic cod (gadus morhua l.): molecular characterization, promoter structure and function analysis |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/163410 http://ir.qdio.ac.cn/handle/337002/163411 https://doi.org/10.1016/j.fsi.2019.07.021 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/163410 http://ir.qdio.ac.cn/handle/337002/163411 doi:10.1016/j.fsi.2019.07.021 |
op_doi |
https://doi.org/10.1016/j.fsi.2019.07.021 |
container_title |
Fish & Shellfish Immunology |
container_volume |
93 |
container_start_page |
28 |
op_container_end_page |
38 |
_version_ |
1766357386010820608 |