Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus
Artificial tetraploid induction is one of the important techniques of fish chromosome manipulation, and it is the first step for triploid breeding. There are a few reports to artificial induction of tetraploid in marine fish. The induction and survival rates were usually low. We firstly optimized th...
Published in: | Frontiers in Marine Science |
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Language: | English |
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FRONTIERS MEDIA SA
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Online Access: | http://ir.qdio.ac.cn/handle/337002/163252 https://doi.org/10.3389/fmars.2019.00637 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/163252 2023-05-15T18:15:43+02:00 Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus Wu, Zhihao Wang, Lijuan Lu, Yunliang Zhu, Xiangping Yue, Xinlu You, Feng 2019-10-16 http://ir.qdio.ac.cn/handle/337002/163252 https://doi.org/10.3389/fmars.2019.00637 英语 eng FRONTIERS MEDIA SA FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/163252 doi:10.3389/fmars.2019.00637 turbot Scophthalmus maximus tetraploid artificial induction first cleavage index genetic diversity Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences HYDROSTATIC-PRESSURE SHOCK ONCORHYNCHUS-MYKISS EMBRYOS RAINBOW-TROUT 1ST CLEAVAGE HEAT-SHOCK GYNOGENESIS POLYPLOIDY CARP HYBRIDIZATION MANIPULATION 期刊论文 2019 ftchinacasciocas https://doi.org/10.3389/fmars.2019.00637 2022-06-27T05:41:18Z Artificial tetraploid induction is one of the important techniques of fish chromosome manipulation, and it is the first step for triploid breeding. There are a few reports to artificial induction of tetraploid in marine fish. The induction and survival rates were usually low. We firstly optimized the tetraploid induction conditions in turbot Scophthalmus maximus, one of the most important maricultural fish in China and Europe. For the initiate time of treatment, which is the most important factor in tetraploid induction, the first cleavage index (FCI) was used to reduce the influences of genetic origin and environment factors. Overall, the optimal initiation time for pressure shock was 15 min before the first cleavage at 14.8-15.5 degrees C. The optimal treatment pressure and treatment duration were 67.5 MPa and 6 min. The regression equation prediction model was: The optimal initiation time = 0.982 FCI - 12.182 or the optimal initiation time = 0.85 FCI. Then two tetraploid induction (4n1 and 4n2) populations were obtained under the optimal conditions with diploid controls (2n1 and 2n2). The induction rates in tetraploid induction (4n) populations at hatched larvae stage could reach 100%. The genetic structure of these two 4n populations was also studied. Two to four alleles in each locus were detected in diploid (2n) and 4n populations, respectively. Private alleles were only appeared at locus Sma-USC21, with two alleles lost in 4n populations. Eleven and fourteen loci in 2n and 4n populations respectively showed a negative genetic deviation index. 3D-FCA analysis showed that the two 2n and two 4n populations have obvious differences. The numbers of locus deviating from Hardy-Weinberg equilibrium in 2n1, 4n1, 2n2, and 4n2 populations were 6, 9, 12, and 7, respectively. Overall, 12 loci in either 2n or 4n population deviated from Hardy-Weinberg equilibrium. Tetraploid induction population showed lower heterozygosity and higher heterozygote deletion. Report Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Frontiers in Marine Science 6 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
turbot Scophthalmus maximus tetraploid artificial induction first cleavage index genetic diversity Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences HYDROSTATIC-PRESSURE SHOCK ONCORHYNCHUS-MYKISS EMBRYOS RAINBOW-TROUT 1ST CLEAVAGE HEAT-SHOCK GYNOGENESIS POLYPLOIDY CARP HYBRIDIZATION MANIPULATION |
spellingShingle |
turbot Scophthalmus maximus tetraploid artificial induction first cleavage index genetic diversity Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences HYDROSTATIC-PRESSURE SHOCK ONCORHYNCHUS-MYKISS EMBRYOS RAINBOW-TROUT 1ST CLEAVAGE HEAT-SHOCK GYNOGENESIS POLYPLOIDY CARP HYBRIDIZATION MANIPULATION Wu, Zhihao Wang, Lijuan Lu, Yunliang Zhu, Xiangping Yue, Xinlu You, Feng Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus |
topic_facet |
turbot Scophthalmus maximus tetraploid artificial induction first cleavage index genetic diversity Environmental Sciences & Ecology Marine & Freshwater Biology Environmental Sciences HYDROSTATIC-PRESSURE SHOCK ONCORHYNCHUS-MYKISS EMBRYOS RAINBOW-TROUT 1ST CLEAVAGE HEAT-SHOCK GYNOGENESIS POLYPLOIDY CARP HYBRIDIZATION MANIPULATION |
description |
Artificial tetraploid induction is one of the important techniques of fish chromosome manipulation, and it is the first step for triploid breeding. There are a few reports to artificial induction of tetraploid in marine fish. The induction and survival rates were usually low. We firstly optimized the tetraploid induction conditions in turbot Scophthalmus maximus, one of the most important maricultural fish in China and Europe. For the initiate time of treatment, which is the most important factor in tetraploid induction, the first cleavage index (FCI) was used to reduce the influences of genetic origin and environment factors. Overall, the optimal initiation time for pressure shock was 15 min before the first cleavage at 14.8-15.5 degrees C. The optimal treatment pressure and treatment duration were 67.5 MPa and 6 min. The regression equation prediction model was: The optimal initiation time = 0.982 FCI - 12.182 or the optimal initiation time = 0.85 FCI. Then two tetraploid induction (4n1 and 4n2) populations were obtained under the optimal conditions with diploid controls (2n1 and 2n2). The induction rates in tetraploid induction (4n) populations at hatched larvae stage could reach 100%. The genetic structure of these two 4n populations was also studied. Two to four alleles in each locus were detected in diploid (2n) and 4n populations, respectively. Private alleles were only appeared at locus Sma-USC21, with two alleles lost in 4n populations. Eleven and fourteen loci in 2n and 4n populations respectively showed a negative genetic deviation index. 3D-FCA analysis showed that the two 2n and two 4n populations have obvious differences. The numbers of locus deviating from Hardy-Weinberg equilibrium in 2n1, 4n1, 2n2, and 4n2 populations were 6, 9, 12, and 7, respectively. Overall, 12 loci in either 2n or 4n population deviated from Hardy-Weinberg equilibrium. Tetraploid induction population showed lower heterozygosity and higher heterozygote deletion. |
format |
Report |
author |
Wu, Zhihao Wang, Lijuan Lu, Yunliang Zhu, Xiangping Yue, Xinlu You, Feng |
author_facet |
Wu, Zhihao Wang, Lijuan Lu, Yunliang Zhu, Xiangping Yue, Xinlu You, Feng |
author_sort |
Wu, Zhihao |
title |
Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus |
title_short |
Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus |
title_full |
Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus |
title_fullStr |
Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus |
title_full_unstemmed |
Artificial Induction and Genetic Structure Analysis of Tetraploid Turbot Scophthalmus maximus |
title_sort |
artificial induction and genetic structure analysis of tetraploid turbot scophthalmus maximus |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/163252 https://doi.org/10.3389/fmars.2019.00637 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FRONTIERS IN MARINE SCIENCE http://ir.qdio.ac.cn/handle/337002/163252 doi:10.3389/fmars.2019.00637 |
op_doi |
https://doi.org/10.3389/fmars.2019.00637 |
container_title |
Frontiers in Marine Science |
container_volume |
6 |
_version_ |
1766188919428218880 |