Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China
Investigating the physiological mechanisms of closely related species that exhibit distinct geographic distributions and thermal niches is essential for understanding their thermal tolerance capacities and local adaptations in view of climate warming. The variations in upper thermal limits (LT50) un...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/162718 2023-05-15T15:58:28+02:00 Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China Ghaffari, Hamze Wang, Wei Li, Ao Zhang, Guofan Li, Li 2019-09-10 http://ir.qdio.ac.cn/handle/337002/162717 http://ir.qdio.ac.cn/handle/337002/162718 https://doi.org/10.3389/fphys.2019.01137 英语 eng FRONTIERS MEDIA SA FRONTIERS IN PHYSIOLOGY http://ir.qdio.ac.cn/handle/337002/162717 http://ir.qdio.ac.cn/handle/337002/162718 doi:10.3389/fphys.2019.01137 thermal tolerance heart rate metabolism physiological performance adaptive variation Physiology 期刊论文 2019 ftchinacasciocas https://doi.org/10.3389/fphys.2019.01137 2022-06-27T05:41:09Z Investigating the physiological mechanisms of closely related species that exhibit distinct geographic distributions and thermal niches is essential for understanding their thermal tolerance capacities and local adaptations in view of climate warming. The variations in upper thermal limits (LT50) under acute heat shock and cardiac activity, standard metabolic rate (SMR), anaerobic metabolite production and molecular responses (expression of molecular chaperones and glycolysis metabolism genes) under increasing temperatures in two oyster subspecies were studied. The populations of two oyster subspecies, Crassostrea gigas gigas and C. gigas angulata, exhibit different latitudinal distributions along the northern and southern coastlines of China, respectively, which experience different environmental conditions. The LT50 was significantly higher, by similar to 1 degrees C, in the southern than in the northern oysters. In both subspecies, temperature increases had powerful effects on heart rate, SMR and gene expression. The southern oysters had the highest Arrhenius breakpoint temperatures for heart rate (31.4 +/- 0.17 degrees C) and SMR (33.09 degrees C), whereas the heart rate (28.86 +/- 0.3 degrees C) and SMR (29.22 degrees C) of the northern oysters were lower. The same patterns were observed for the Q(10) coefficients. More thermal sensitivity was observed in the northern oysters than in their southern counterparts, as the heat-shock proteins (HSPs) in the northern oysters were expressed first and had a higher induction at a lower temperature than those of southern oysters. Furthermore, different expression patterns of energetic metabolism genes (HK, PK, and PEPCK) were observed. In the northern oysters, increasing anaerobic glycolysis genes (PEPCK) and end products (succinate) were found at 36-43 degrees C, indicating a transition from aerobic to anaerobic metabolism and a lower aerobic scope compared with the southern oysters. These two subspecies experience different environmental conditions, and their ... Report Crassostrea gigas Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Frontiers in Physiology 10 |
institution |
Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
thermal tolerance heart rate metabolism physiological performance adaptive variation Physiology |
spellingShingle |
thermal tolerance heart rate metabolism physiological performance adaptive variation Physiology Ghaffari, Hamze Wang, Wei Li, Ao Zhang, Guofan Li, Li Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China |
topic_facet |
thermal tolerance heart rate metabolism physiological performance adaptive variation Physiology |
description |
Investigating the physiological mechanisms of closely related species that exhibit distinct geographic distributions and thermal niches is essential for understanding their thermal tolerance capacities and local adaptations in view of climate warming. The variations in upper thermal limits (LT50) under acute heat shock and cardiac activity, standard metabolic rate (SMR), anaerobic metabolite production and molecular responses (expression of molecular chaperones and glycolysis metabolism genes) under increasing temperatures in two oyster subspecies were studied. The populations of two oyster subspecies, Crassostrea gigas gigas and C. gigas angulata, exhibit different latitudinal distributions along the northern and southern coastlines of China, respectively, which experience different environmental conditions. The LT50 was significantly higher, by similar to 1 degrees C, in the southern than in the northern oysters. In both subspecies, temperature increases had powerful effects on heart rate, SMR and gene expression. The southern oysters had the highest Arrhenius breakpoint temperatures for heart rate (31.4 +/- 0.17 degrees C) and SMR (33.09 degrees C), whereas the heart rate (28.86 +/- 0.3 degrees C) and SMR (29.22 degrees C) of the northern oysters were lower. The same patterns were observed for the Q(10) coefficients. More thermal sensitivity was observed in the northern oysters than in their southern counterparts, as the heat-shock proteins (HSPs) in the northern oysters were expressed first and had a higher induction at a lower temperature than those of southern oysters. Furthermore, different expression patterns of energetic metabolism genes (HK, PK, and PEPCK) were observed. In the northern oysters, increasing anaerobic glycolysis genes (PEPCK) and end products (succinate) were found at 36-43 degrees C, indicating a transition from aerobic to anaerobic metabolism and a lower aerobic scope compared with the southern oysters. These two subspecies experience different environmental conditions, and their ... |
format |
Report |
author |
Ghaffari, Hamze Wang, Wei Li, Ao Zhang, Guofan Li, Li |
author_facet |
Ghaffari, Hamze Wang, Wei Li, Ao Zhang, Guofan Li, Li |
author_sort |
Ghaffari, Hamze |
title |
Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China |
title_short |
Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China |
title_full |
Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China |
title_fullStr |
Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China |
title_full_unstemmed |
Thermotolerance Divergence Revealed by the Physiological and Molecular Responses in Two Oyster Subspecies of Crassostrea gigas in China |
title_sort |
thermotolerance divergence revealed by the physiological and molecular responses in two oyster subspecies of crassostrea gigas in china |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/162717 http://ir.qdio.ac.cn/handle/337002/162718 https://doi.org/10.3389/fphys.2019.01137 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
FRONTIERS IN PHYSIOLOGY http://ir.qdio.ac.cn/handle/337002/162717 http://ir.qdio.ac.cn/handle/337002/162718 doi:10.3389/fphys.2019.01137 |
op_doi |
https://doi.org/10.3389/fphys.2019.01137 |
container_title |
Frontiers in Physiology |
container_volume |
10 |
_version_ |
1766394221254672384 |