Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity
Myogenic regulatory factor 4 (MRF4) is a basic helix-loop-helix (bHLH) transcription factor that plays crucial roles in myoblast differentiation and maturation. Here, we report the isolation of the olive flounder (Paralichthys olivaceus) mrf4 gene and the spatiotemporal analysis of its expression pa...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/162702 http://ir.qdio.ac.cn/handle/337002/162703 https://doi.org/10.1016/j.cbpb.2019.110310 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/162703 2023-05-15T18:09:53+02:00 Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity Tan, Xungang Xu, Peng Zhang, Yuqing Zhang, Pei-Jun 2019-10-01 http://ir.qdio.ac.cn/handle/337002/162702 http://ir.qdio.ac.cn/handle/337002/162703 https://doi.org/10.1016/j.cbpb.2019.110310 英语 eng ELSEVIER SCIENCE INC COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY http://ir.qdio.ac.cn/handle/337002/162702 http://ir.qdio.ac.cn/handle/337002/162703 doi:10.1016/j.cbpb.2019.110310 Olive flounder Myogenesis Somites mrf4 promoter Zebrafish Biochemistry & Molecular Biology Zoology 期刊论文 2019 ftchinacasciocas https://doi.org/10.1016/j.cbpb.2019.110310 2022-06-27T05:41:09Z Myogenic regulatory factor 4 (MRF4) is a basic helix-loop-helix (bHLH) transcription factor that plays crucial roles in myoblast differentiation and maturation. Here, we report the isolation of the olive flounder (Paralichthys olivaceus) mrf4 gene and the spatiotemporal analysis of its expression patterns. Sequence analysis indicated that flounder mrf4 shared a similar structure with other vertebrate MRF4, including the conserved bHLH domain. Flounder mrf4 contains 3 exons and 2 introns. Sequence alignment and phylogenetic analysis showed that it was highly homologous with Salmo salar, Danio rerio, Takifugu rubripes, and Tetraodon nigroviridis mrf4. Flounder mrf4 was first expressed in the medial region of somites that give rise to slow muscles, and later spread to the lateral region of somites that give rise to fast muscles. Mrf4 transcript levels decreased significantly in mature somites in the trunk region, and expression could only be detected in the caudal somites, consistent with the timing of somite maturation. Transient expression analysis showed that the 506 bp flounder mrf4 promoter was sufficient to direct muscle-specific GFP expression in zebrafish embryos. Report Salmo salar Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 236 110310 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Olive flounder Myogenesis Somites mrf4 promoter Zebrafish Biochemistry & Molecular Biology Zoology |
spellingShingle |
Olive flounder Myogenesis Somites mrf4 promoter Zebrafish Biochemistry & Molecular Biology Zoology Tan, Xungang Xu, Peng Zhang, Yuqing Zhang, Pei-Jun Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity |
topic_facet |
Olive flounder Myogenesis Somites mrf4 promoter Zebrafish Biochemistry & Molecular Biology Zoology |
description |
Myogenic regulatory factor 4 (MRF4) is a basic helix-loop-helix (bHLH) transcription factor that plays crucial roles in myoblast differentiation and maturation. Here, we report the isolation of the olive flounder (Paralichthys olivaceus) mrf4 gene and the spatiotemporal analysis of its expression patterns. Sequence analysis indicated that flounder mrf4 shared a similar structure with other vertebrate MRF4, including the conserved bHLH domain. Flounder mrf4 contains 3 exons and 2 introns. Sequence alignment and phylogenetic analysis showed that it was highly homologous with Salmo salar, Danio rerio, Takifugu rubripes, and Tetraodon nigroviridis mrf4. Flounder mrf4 was first expressed in the medial region of somites that give rise to slow muscles, and later spread to the lateral region of somites that give rise to fast muscles. Mrf4 transcript levels decreased significantly in mature somites in the trunk region, and expression could only be detected in the caudal somites, consistent with the timing of somite maturation. Transient expression analysis showed that the 506 bp flounder mrf4 promoter was sufficient to direct muscle-specific GFP expression in zebrafish embryos. |
format |
Report |
author |
Tan, Xungang Xu, Peng Zhang, Yuqing Zhang, Pei-Jun |
author_facet |
Tan, Xungang Xu, Peng Zhang, Yuqing Zhang, Pei-Jun |
author_sort |
Tan, Xungang |
title |
Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity |
title_short |
Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity |
title_full |
Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity |
title_fullStr |
Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity |
title_full_unstemmed |
Olive flounder (Paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity |
title_sort |
olive flounder (paralichthys olivaceus) myogenic regulatory factor 4 and its muscle-specific promoter activity |
publisher |
ELSEVIER SCIENCE INC |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/162702 http://ir.qdio.ac.cn/handle/337002/162703 https://doi.org/10.1016/j.cbpb.2019.110310 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY http://ir.qdio.ac.cn/handle/337002/162702 http://ir.qdio.ac.cn/handle/337002/162703 doi:10.1016/j.cbpb.2019.110310 |
op_doi |
https://doi.org/10.1016/j.cbpb.2019.110310 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
236 |
container_start_page |
110310 |
_version_ |
1766182576000598016 |