The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas
Thyroid hormones (THs) are indispensable for each phyla in Chordata, while their functions in the non-chordate invertebrates are indistinct. Studies on the TH system in non-chordate invertebrates are important for understanding the evolution of TH system and may be applied in aquaculture or biofouli...
Published in: | Journal of Oceanology and Limnology |
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Online Access: | http://ir.qdio.ac.cn/handle/337002/162128 https://doi.org/10.1007/s00343-019-8207-9 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/162128 2023-05-15T15:58:12+02:00 The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas Huang Wen Xu Fei Li Li Que Huayong Zhang Guofan 2019-07-01 http://ir.qdio.ac.cn/handle/337002/162128 https://doi.org/10.1007/s00343-019-8207-9 英语 eng SCIENCE PRESS JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.qdio.ac.cn/handle/337002/162128 doi:10.1007/s00343-019-8207-9 iodothyronine deiodinase mollusk thyroid hormone (TH) TH responsive elements Marine & Freshwater Biology Oceanography Limnology REVEALS TRIIODOTHYRONINE MECHANISM GROWTH 期刊论文 2019 ftchinacasciocas https://doi.org/10.1007/s00343-019-8207-9 2022-06-27T05:40:58Z Thyroid hormones (THs) are indispensable for each phyla in Chordata, while their functions in the non-chordate invertebrates are indistinct. Studies on the TH system in non-chordate invertebrates are important for understanding the evolution of TH system and may be applied in aquaculture or biofouling control at the same time. Iodothyronine deiodinases are keys to studying the TH system, as they are critical enzymes in maintaining TH homeostasis by catalyzing the initiation and termination of the effects of thyroid hormone in vertebrates. Here, we report the primary physiological effects of T4, the outer ring deiodinase activity, and a similar transcription regulation of two oyster deiodinases by TH receptor (CgTR) in an invertebrate, Pacific oyster Crassostrea gigas. L-thyroxine (T4) may have an important physiological function in the oyster, suggested by the growth retardation effect of excessive T4 in umbo larvae stage. The outer ring deiodinase activity transforming T4 to T3 (3, 3', 5-triiodothyronine) was then detected in the Pacific oyster in vivo, which may be conducted by two oyster deiodinases (CgDx and CgDy). Transcription regulation of CgTR onto these two deiodinase genes was also verified by electrophoretic mobility shift assay and dual luciferase reporter assay in mammalian cells. These results contribute to a better understanding of the evolution of the TH system. Report Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Journal of Oceanology and Limnology 37 4 1317 1323 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
iodothyronine deiodinase mollusk thyroid hormone (TH) TH responsive elements Marine & Freshwater Biology Oceanography Limnology REVEALS TRIIODOTHYRONINE MECHANISM GROWTH |
spellingShingle |
iodothyronine deiodinase mollusk thyroid hormone (TH) TH responsive elements Marine & Freshwater Biology Oceanography Limnology REVEALS TRIIODOTHYRONINE MECHANISM GROWTH Huang Wen Xu Fei Li Li Que Huayong Zhang Guofan The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas |
topic_facet |
iodothyronine deiodinase mollusk thyroid hormone (TH) TH responsive elements Marine & Freshwater Biology Oceanography Limnology REVEALS TRIIODOTHYRONINE MECHANISM GROWTH |
description |
Thyroid hormones (THs) are indispensable for each phyla in Chordata, while their functions in the non-chordate invertebrates are indistinct. Studies on the TH system in non-chordate invertebrates are important for understanding the evolution of TH system and may be applied in aquaculture or biofouling control at the same time. Iodothyronine deiodinases are keys to studying the TH system, as they are critical enzymes in maintaining TH homeostasis by catalyzing the initiation and termination of the effects of thyroid hormone in vertebrates. Here, we report the primary physiological effects of T4, the outer ring deiodinase activity, and a similar transcription regulation of two oyster deiodinases by TH receptor (CgTR) in an invertebrate, Pacific oyster Crassostrea gigas. L-thyroxine (T4) may have an important physiological function in the oyster, suggested by the growth retardation effect of excessive T4 in umbo larvae stage. The outer ring deiodinase activity transforming T4 to T3 (3, 3', 5-triiodothyronine) was then detected in the Pacific oyster in vivo, which may be conducted by two oyster deiodinases (CgDx and CgDy). Transcription regulation of CgTR onto these two deiodinase genes was also verified by electrophoretic mobility shift assay and dual luciferase reporter assay in mammalian cells. These results contribute to a better understanding of the evolution of the TH system. |
format |
Report |
author |
Huang Wen Xu Fei Li Li Que Huayong Zhang Guofan |
author_facet |
Huang Wen Xu Fei Li Li Que Huayong Zhang Guofan |
author_sort |
Huang Wen |
title |
The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas |
title_short |
The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas |
title_full |
The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas |
title_fullStr |
The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the Pacific oyster Crassostrea gigas |
title_sort |
transcription of iodothyronine deiodinase genes is regulated by thyroid hormone receptor in the pacific oyster crassostrea gigas |
publisher |
SCIENCE PRESS |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/162128 https://doi.org/10.1007/s00343-019-8207-9 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.qdio.ac.cn/handle/337002/162128 doi:10.1007/s00343-019-8207-9 |
op_doi |
https://doi.org/10.1007/s00343-019-8207-9 |
container_title |
Journal of Oceanology and Limnology |
container_volume |
37 |
container_issue |
4 |
container_start_page |
1317 |
op_container_end_page |
1323 |
_version_ |
1766393934329675776 |