Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae
Light is a key environmental factor that synchronizes various life stages from embryo development to sexual maturation in fish. For turbot, light spectra have the most influence at the larval and juvenile stages. In the current study, differences in the development of embryos and the performance of...
Published in: | Fish & Shellfish Immunology |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2019
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Online Access: | http://ir.qdio.ac.cn/handle/337002/161779 https://doi.org/10.1016/j.fsi.2019.05.007 |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/161779 2023-05-15T16:19:25+02:00 Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae Wu, Lele Han, Mingming Song, Zongcheng Xu, Shihong Li, Jun Li, Xueqing Wang, Yanfeng Yue, Xinlu Li, Xian 2019-07-01 http://ir.qdio.ac.cn/handle/337002/161779 https://doi.org/10.1016/j.fsi.2019.05.007 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/161779 doi:10.1016/j.fsi.2019.05.007 Scophthalmus maximus Light spectrum Embryo Newly hatched larvae Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences INNATE IMMUNE-RESPONSES OSTEOLOGICAL DEVELOPMENT OXIDATIVE STRESS GENE-EXPRESSION SPARUS-AURATA OPLEGNATHUS-FASCIATUS MOLECULAR-CLONING BACKGROUND COLOR JAW DEVELOPMENT GADUS-MORHUA 期刊论文 2019 ftchinacasciocas https://doi.org/10.1016/j.fsi.2019.05.007 2022-06-27T05:40:50Z Light is a key environmental factor that synchronizes various life stages from embryo development to sexual maturation in fish. For turbot, light spectra have the most influence at the larval and juvenile stages. In the current study, differences in the development of embryos and the performance of newly hatched turbot larvae exposed to five different spectra: full spectrum (LDF), blue (LOB, peak at 450 nm), green (LDG, peak at 533 nm), orange (LDO, peak at 595 nm) and red (LDR, peak at 629 nm), were examined. At 62.8 h post fertilization, a higher number of embryos exposed to short-wavelengths (LDG and LOB) had developed a heartbeat in comparison with embryos exposed to other wavelengths. Larvae exposed to the green spectrum had higher malformation rates than larvae exposed to the other spectra, indicating that larvae exposed to green light may have significantly reduced survival rates. The results of non-specific immunity parameters showed that the mRNA expression levels of cathepsin D (CTSD), cathepsin F (CTSF), catalase (CAT) and metallothionein (MT) in larvae exposed to LDB were significantly higher than those exposed to other spectra, but CAT activity in larvae exposed to LDB was significantly lower than larvae exposed to the other spectra. There was no significant difference in MT activity in larvae exposed to the five different spectra. The mRNA expression level of lysozyme (LZM) in larvae exposed to LDR was significantly higher than other spectra, while there was no significant difference in LZM activity observed in larvae exposed to LDR, LDG, LDB and LDF. The difference of the enzyme activity of total superoxide dismutase (T-SOD) was not significant among larvae exposed to the five spectra. mRNA expression of the heat shock protein 70 (HSP70) was significantly higher in newly hatched larvae exposed to LDB, LDR and LDG, indicating that larvae exposed to LDB, LDG and LDR exhibited a stress response. The mRNA expression level of the insulin-like growth factor-1 (IGF-1) and growth parameters in the newly ... Report Gadus morhua Scophthalmus maximus Turbot Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 90 328 337 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Scophthalmus maximus Light spectrum Embryo Newly hatched larvae Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences INNATE IMMUNE-RESPONSES OSTEOLOGICAL DEVELOPMENT OXIDATIVE STRESS GENE-EXPRESSION SPARUS-AURATA OPLEGNATHUS-FASCIATUS MOLECULAR-CLONING BACKGROUND COLOR JAW DEVELOPMENT GADUS-MORHUA |
spellingShingle |
Scophthalmus maximus Light spectrum Embryo Newly hatched larvae Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences INNATE IMMUNE-RESPONSES OSTEOLOGICAL DEVELOPMENT OXIDATIVE STRESS GENE-EXPRESSION SPARUS-AURATA OPLEGNATHUS-FASCIATUS MOLECULAR-CLONING BACKGROUND COLOR JAW DEVELOPMENT GADUS-MORHUA Wu, Lele Han, Mingming Song, Zongcheng Xu, Shihong Li, Jun Li, Xueqing Wang, Yanfeng Yue, Xinlu Li, Xian Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae |
topic_facet |
Scophthalmus maximus Light spectrum Embryo Newly hatched larvae Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences INNATE IMMUNE-RESPONSES OSTEOLOGICAL DEVELOPMENT OXIDATIVE STRESS GENE-EXPRESSION SPARUS-AURATA OPLEGNATHUS-FASCIATUS MOLECULAR-CLONING BACKGROUND COLOR JAW DEVELOPMENT GADUS-MORHUA |
description |
Light is a key environmental factor that synchronizes various life stages from embryo development to sexual maturation in fish. For turbot, light spectra have the most influence at the larval and juvenile stages. In the current study, differences in the development of embryos and the performance of newly hatched turbot larvae exposed to five different spectra: full spectrum (LDF), blue (LOB, peak at 450 nm), green (LDG, peak at 533 nm), orange (LDO, peak at 595 nm) and red (LDR, peak at 629 nm), were examined. At 62.8 h post fertilization, a higher number of embryos exposed to short-wavelengths (LDG and LOB) had developed a heartbeat in comparison with embryos exposed to other wavelengths. Larvae exposed to the green spectrum had higher malformation rates than larvae exposed to the other spectra, indicating that larvae exposed to green light may have significantly reduced survival rates. The results of non-specific immunity parameters showed that the mRNA expression levels of cathepsin D (CTSD), cathepsin F (CTSF), catalase (CAT) and metallothionein (MT) in larvae exposed to LDB were significantly higher than those exposed to other spectra, but CAT activity in larvae exposed to LDB was significantly lower than larvae exposed to the other spectra. There was no significant difference in MT activity in larvae exposed to the five different spectra. The mRNA expression level of lysozyme (LZM) in larvae exposed to LDR was significantly higher than other spectra, while there was no significant difference in LZM activity observed in larvae exposed to LDR, LDG, LDB and LDF. The difference of the enzyme activity of total superoxide dismutase (T-SOD) was not significant among larvae exposed to the five spectra. mRNA expression of the heat shock protein 70 (HSP70) was significantly higher in newly hatched larvae exposed to LDB, LDR and LDG, indicating that larvae exposed to LDB, LDG and LDR exhibited a stress response. The mRNA expression level of the insulin-like growth factor-1 (IGF-1) and growth parameters in the newly ... |
format |
Report |
author |
Wu, Lele Han, Mingming Song, Zongcheng Xu, Shihong Li, Jun Li, Xueqing Wang, Yanfeng Yue, Xinlu Li, Xian |
author_facet |
Wu, Lele Han, Mingming Song, Zongcheng Xu, Shihong Li, Jun Li, Xueqing Wang, Yanfeng Yue, Xinlu Li, Xian |
author_sort |
Wu, Lele |
title |
Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae |
title_short |
Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae |
title_full |
Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae |
title_fullStr |
Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae |
title_full_unstemmed |
Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae |
title_sort |
effects of different light spectra on embryo development and the performance of newly hatched turbot (scophthalmus maximus) larvae |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2019 |
url |
http://ir.qdio.ac.cn/handle/337002/161779 https://doi.org/10.1016/j.fsi.2019.05.007 |
genre |
Gadus morhua Scophthalmus maximus Turbot |
genre_facet |
Gadus morhua Scophthalmus maximus Turbot |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.qdio.ac.cn/handle/337002/161779 doi:10.1016/j.fsi.2019.05.007 |
op_doi |
https://doi.org/10.1016/j.fsi.2019.05.007 |
container_title |
Fish & Shellfish Immunology |
container_volume |
90 |
container_start_page |
328 |
op_container_end_page |
337 |
_version_ |
1766005817269551104 |