Network of microRNA-transcriptional factor-mRNA in cold response of turbot Scophthalmus maximus

• Published in: Fish Physiology and Biochemistry
• Main Authors: Nie, Miaomiao, Tan, Xungang, Lu, Yunliang, Wu, Zhihao, Li, Jun, Xu, Dongdong, Zhang, Peijun, You, Feng
• Format: Report
• Language: English
• Published: SPRINGER 2019
• Subjects: Scophthalmus maximus; Cold tolerance; Transcription; microRNA; Transcription factor; Biochemistry & Molecular Biology; Fisheries; Physiology; Turbot
• Online Access: http://ir.qdio.ac.cn/handle/337002/161610; http://ir.qdio.ac.cn/handle/337002/161611; https://doi.org/10.1007/s10695-019-00611-y

The aim of this study is to understand fish cold-tolerant mechanism. We analyzed the transcriptional reactions to the cold condition in turbot Scophthalmus maximus by using RNA-seq and microRNA (miRNA)-seq. Meio-gynogenetic diploid turbots were treated at 0 degrees C to distinguish the cold-tolerant (CT) and cold-sensitive (CS) groups. The results showed that there were quite different responses at both mRNA and miRNA levels, with more up-regulated mRNAs (1069 vs. 194) and less down-regulated miRNAs (4 vs. 1) in CT versus CS relative to the control group. The network of miRNA-transcription factor-mRNA, regulating turbot different response to cold stress, was constructed, which involved in cell cycle, component of cell membrane, signal transduction, and circadian rhythm pathways. The above information demonstrates mechanisms by which cold tolerance is increased in fish.